Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus)
The effect of cold storage of testes and epididymides at 4 °C for 12 h on the cryopreservation capacity of epididymal feline sperm was evaluated. Ten domestic cats were castrated, and testes and epididymides collected. Specimens were randomly assigned to two groups: in Group A, epididymal samples we...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2017-03-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201703-0005_effects-of-epididymis-cold-storage-on-frozen-thawed-epididymal-sperm-quality-in-tomcats-felis-catus.php |
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| author | C.C. Perez-Marin E. Jimenez E.I. Aguera |
| author_facet | C.C. Perez-Marin E. Jimenez E.I. Aguera |
| author_sort | C.C. Perez-Marin |
| collection | DOAJ |
| description | The effect of cold storage of testes and epididymides at 4 °C for 12 h on the cryopreservation capacity of epididymal feline sperm was evaluated. Ten domestic cats were castrated, and testes and epididymides collected. Specimens were randomly assigned to two groups: in Group A, epididymal samples were immediately processed and frozen in 0.25-ml straws; in Group B, both testes and epididymides were maintained in saline at 4 °C for 12 h and sperm was then processed and frozen. Motility, morphology, acrosome status, sperm viability and DNA integrity were assessed in epididymal sperm samples before freezing (baseline), at thawing (0 h) and 6 h post-thawing (6 h). Although values were lower in Group B, no significant intergroup difference was observed for any of the parameters tested either at baseline or at 0 h. However, significantly higher values (P < 0.05) were observed in Group A at 6 h for total sperm motility (29.0 ± 2.4% vs 13.0 ± 4.3%), sperm viability (35.2 ± 5.4% vs 15.4 ± 1.4%) and normal morphology (47.6 ± 0.8% vs 40.0 ± 2.1%). It was observed that motility and acrosome status of epididymal sperm are the most sensitive parameters when both types of sperm samples (from fresh epididymis or from 12 h cold-stored epididymis) are frozen-thawed. When sperm quality was assessed 6 h after thawing, spermatozoa precooled in the epididymides showed significantly lower values for motility, viability and morphology than spermatozoa from fresh epididymal samples. |
| first_indexed | 2024-04-10T07:58:09Z |
| format | Article |
| id | doaj.art-08ad68c71680455da0cc064f7bcad7f7 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:58:09Z |
| publishDate | 2017-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-08ad68c71680455da0cc064f7bcad7f72023-02-23T03:50:40ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922017-03-0162314715210.17221/253/2015-VETMEDvet-201703-0005Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus)C.C. Perez-MarinE. Jimenez0E.I. Aguera1Faculty of Veterinary Medicine, University of Cordoba, Cordoba, SpainFaculty of Veterinary Medicine, University of Cordoba, Cordoba, SpainThe effect of cold storage of testes and epididymides at 4 °C for 12 h on the cryopreservation capacity of epididymal feline sperm was evaluated. Ten domestic cats were castrated, and testes and epididymides collected. Specimens were randomly assigned to two groups: in Group A, epididymal samples were immediately processed and frozen in 0.25-ml straws; in Group B, both testes and epididymides were maintained in saline at 4 °C for 12 h and sperm was then processed and frozen. Motility, morphology, acrosome status, sperm viability and DNA integrity were assessed in epididymal sperm samples before freezing (baseline), at thawing (0 h) and 6 h post-thawing (6 h). Although values were lower in Group B, no significant intergroup difference was observed for any of the parameters tested either at baseline or at 0 h. However, significantly higher values (P < 0.05) were observed in Group A at 6 h for total sperm motility (29.0 ± 2.4% vs 13.0 ± 4.3%), sperm viability (35.2 ± 5.4% vs 15.4 ± 1.4%) and normal morphology (47.6 ± 0.8% vs 40.0 ± 2.1%). It was observed that motility and acrosome status of epididymal sperm are the most sensitive parameters when both types of sperm samples (from fresh epididymis or from 12 h cold-stored epididymis) are frozen-thawed. When sperm quality was assessed 6 h after thawing, spermatozoa precooled in the epididymides showed significantly lower values for motility, viability and morphology than spermatozoa from fresh epididymal samples.https://vetmed.agriculturejournals.cz/artkey/vet-201703-0005_effects-of-epididymis-cold-storage-on-frozen-thawed-epididymal-sperm-quality-in-tomcats-felis-catus.phpfelinespermatozoacryopreservationcooling |
| spellingShingle | C.C. Perez-Marin E. Jimenez E.I. Aguera Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus) Veterinární Medicína feline spermatozoa cryopreservation cooling |
| title | Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus) |
| title_full | Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus) |
| title_fullStr | Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus) |
| title_full_unstemmed | Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus) |
| title_short | Effects of epididymis cold storage on frozen-thawed epididymal sperm quality in tomcats (Felis catus) |
| title_sort | effects of epididymis cold storage on frozen thawed epididymal sperm quality in tomcats felis catus |
| topic | feline spermatozoa cryopreservation cooling |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201703-0005_effects-of-epididymis-cold-storage-on-frozen-thawed-epididymal-sperm-quality-in-tomcats-felis-catus.php |
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