Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein
We report on the construction of functionalized nanotubes based on tail sheath protein 041 from vB_KleM-RaK2 bacteriophage. The truncated 041 protein (041Δ200) was fused with fluorescent proteins GFP and mCherry or amidohydrolase YqfB. The generated chimeric proteins were successfully synthesized in...
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MDPI AG
2021-11-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/11/3031 |
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| author | Greta Labutytė Simona Povilonienė Eugenijus Šimoliūnas Dovydas Gabrielaitis Martynas Skapas Algirdas Noreika Rolandas Meškys Vida Časaitė |
| author_facet | Greta Labutytė Simona Povilonienė Eugenijus Šimoliūnas Dovydas Gabrielaitis Martynas Skapas Algirdas Noreika Rolandas Meškys Vida Časaitė |
| author_sort | Greta Labutytė |
| collection | DOAJ |
| description | We report on the construction of functionalized nanotubes based on tail sheath protein 041 from vB_KleM-RaK2 bacteriophage. The truncated 041 protein (041Δ200) was fused with fluorescent proteins GFP and mCherry or amidohydrolase YqfB. The generated chimeric proteins were successfully synthesized in <i>E. coli</i> BL21 (DE3) cells and self-assembled into tubular structures. We detected the fluorescence of the structures, which was confirmed by stimulated emission depletion microscopy. When 041Δ200GFP and 041Δ200mCherry were coexpressed in <i>E. coli</i> BL21 (DE3) cells, the formed nanotubes generated Förster resonance energy transfer, indicating that both fluorescent proteins assemble into a single nanotube. Chimeric 041Δ200YqfB nanotubes possessed an enzymatic activity, which was confirmed by hydrolysis of <i>N</i><sup>4</sup>-acetyl-2′-deoxycytidine. The enzymatic properties of 041Δ200YqfB were similar to those of a free wild-type YqfB. Hence, we conclude that 041-based chimeric nanotubes have the potential for the development of delivery vehicles and targeted imaging and are applicable as scaffolds for biocatalysts. |
| first_indexed | 2024-03-10T05:12:29Z |
| format | Article |
| id | doaj.art-08ae77462d274fb7b2f8279b54ebfe09 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T05:12:29Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-08ae77462d274fb7b2f8279b54ebfe092023-11-23T00:42:08ZengMDPI AGNanomaterials2079-49912021-11-011111303110.3390/nano11113031Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath ProteinGreta Labutytė0Simona Povilonienė1Eugenijus Šimoliūnas2Dovydas Gabrielaitis3Martynas Skapas4Algirdas Noreika5Rolandas Meškys6Vida Časaitė7Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaCenter for Physical Sciences and Technology, Saulėtekio av. 3, LT-10257 Vilnius, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaDepartment of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, LithuaniaWe report on the construction of functionalized nanotubes based on tail sheath protein 041 from vB_KleM-RaK2 bacteriophage. The truncated 041 protein (041Δ200) was fused with fluorescent proteins GFP and mCherry or amidohydrolase YqfB. The generated chimeric proteins were successfully synthesized in <i>E. coli</i> BL21 (DE3) cells and self-assembled into tubular structures. We detected the fluorescence of the structures, which was confirmed by stimulated emission depletion microscopy. When 041Δ200GFP and 041Δ200mCherry were coexpressed in <i>E. coli</i> BL21 (DE3) cells, the formed nanotubes generated Förster resonance energy transfer, indicating that both fluorescent proteins assemble into a single nanotube. Chimeric 041Δ200YqfB nanotubes possessed an enzymatic activity, which was confirmed by hydrolysis of <i>N</i><sup>4</sup>-acetyl-2′-deoxycytidine. The enzymatic properties of 041Δ200YqfB were similar to those of a free wild-type YqfB. Hence, we conclude that 041-based chimeric nanotubes have the potential for the development of delivery vehicles and targeted imaging and are applicable as scaffolds for biocatalysts.https://www.mdpi.com/2079-4991/11/11/3031bacteriophage vB_KleM-RaK2tail sheath proteinnanotubegreen fluorescent proteinmCherryYqfB |
| spellingShingle | Greta Labutytė Simona Povilonienė Eugenijus Šimoliūnas Dovydas Gabrielaitis Martynas Skapas Algirdas Noreika Rolandas Meškys Vida Časaitė Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein Nanomaterials bacteriophage vB_KleM-RaK2 tail sheath protein nanotube green fluorescent protein mCherry YqfB |
| title | Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein |
| title_full | Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein |
| title_fullStr | Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein |
| title_full_unstemmed | Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein |
| title_short | Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein |
| title_sort | functionalized protein nanotubes based on the bacteriophage vb klem rak2 tail sheath protein |
| topic | bacteriophage vB_KleM-RaK2 tail sheath protein nanotube green fluorescent protein mCherry YqfB |
| url | https://www.mdpi.com/2079-4991/11/11/3031 |
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