Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Rift Valley fever (RVF) is a mosquito-borne zoonotic disease that is caused by the Rift Valley fever virus (RVFV); <i>Bunyaviridae</i>: Phlebovirus. RVF disease can affect several different species, including ruminants, camels and humans and thus present a dual threat to public health an...
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MDPI AG
2022-11-01
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Online Access: | https://www.mdpi.com/2075-4418/12/11/2664 |
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author | Thulaganyo Domfe Zikhona Njengele-Tetyana Nikiwe Mhlanga Phumlani Tetyana Amanda Skepu Jane Catherine Ngila Lucky Mashudu Sikhwivhilu |
author_facet | Thulaganyo Domfe Zikhona Njengele-Tetyana Nikiwe Mhlanga Phumlani Tetyana Amanda Skepu Jane Catherine Ngila Lucky Mashudu Sikhwivhilu |
author_sort | Thulaganyo Domfe |
collection | DOAJ |
description | Rift Valley fever (RVF) is a mosquito-borne zoonotic disease that is caused by the Rift Valley fever virus (RVFV); <i>Bunyaviridae</i>: Phlebovirus. RVF disease can affect several different species, including ruminants, camels and humans and thus present a dual threat to public health and livestock food production in endemic regions. In livestock, the RVFV infection is characterised by an acute hepatitis, abortion and high mortality rates in new-born animals. The current RVF diagnostic techniques have shown good sensitivity. However, they require extensive sample processing and complex instrumentation. Owing to speed, low cost, ease of use, and most importantly, the ability to diagnose diseases at sites where they are managed, lateral flow immunoassays (LFIA) are the most widely used point-of-care (POC) tools for disease diagnosis. In this study, a lateral flow assay (LFA) device that is able to detect antibodies against RVFV, with a minimum detectable concentration of 0.125 mg/mL, was successfully developed. The LFA also successfully detected RVFV antibodies in reference RVFV sera. Protein A (ProA), which has the ability to bind immunoglobulins from different species, was used in the detection probe, giving the developed RVFV LFA potential for multi-species diagnosis. |
first_indexed | 2024-03-09T19:08:59Z |
format | Article |
id | doaj.art-08b19ddef8ca4c4abbbf2bacef987966 |
institution | Directory Open Access Journal |
issn | 2075-4418 |
language | English |
last_indexed | 2024-03-09T19:08:59Z |
publishDate | 2022-11-01 |
publisher | MDPI AG |
record_format | Article |
series | Diagnostics |
spelling | doaj.art-08b19ddef8ca4c4abbbf2bacef9879662023-11-24T04:18:58ZengMDPI AGDiagnostics2075-44182022-11-011211266410.3390/diagnostics12112664Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus AntibodiesThulaganyo Domfe0Zikhona Njengele-Tetyana1Nikiwe Mhlanga2Phumlani Tetyana3Amanda Skepu4Jane Catherine Ngila5Lucky Mashudu Sikhwivhilu6Department of Chemical Sciences, University of Johannesburg, Doornfontein, Johannesburg 2050, South AfricaAdvanced Materials Division, DSI/Mintek NIC, Mintek, 200 Malibongwe Drive, Randburg 2194, South AfricaAdvanced Materials Division, DSI/Mintek NIC, Mintek, 200 Malibongwe Drive, Randburg 2194, South AfricaAdvanced Materials Division, DSI/Mintek NIC, Mintek, 200 Malibongwe Drive, Randburg 2194, South AfricaNext Generation Health, Council for Scientific and Industrial Research, Meiring Naude Road, Brummeria, Pretoria 0001, South AfricaDepartment of Chemical Sciences, University of Johannesburg, Doornfontein, Johannesburg 2050, South AfricaAdvanced Materials Division, DSI/Mintek NIC, Mintek, 200 Malibongwe Drive, Randburg 2194, South AfricaRift Valley fever (RVF) is a mosquito-borne zoonotic disease that is caused by the Rift Valley fever virus (RVFV); <i>Bunyaviridae</i>: Phlebovirus. RVF disease can affect several different species, including ruminants, camels and humans and thus present a dual threat to public health and livestock food production in endemic regions. In livestock, the RVFV infection is characterised by an acute hepatitis, abortion and high mortality rates in new-born animals. The current RVF diagnostic techniques have shown good sensitivity. However, they require extensive sample processing and complex instrumentation. Owing to speed, low cost, ease of use, and most importantly, the ability to diagnose diseases at sites where they are managed, lateral flow immunoassays (LFIA) are the most widely used point-of-care (POC) tools for disease diagnosis. In this study, a lateral flow assay (LFA) device that is able to detect antibodies against RVFV, with a minimum detectable concentration of 0.125 mg/mL, was successfully developed. The LFA also successfully detected RVFV antibodies in reference RVFV sera. Protein A (ProA), which has the ability to bind immunoglobulins from different species, was used in the detection probe, giving the developed RVFV LFA potential for multi-species diagnosis.https://www.mdpi.com/2075-4418/12/11/2664Rift Valley fever virusprotein expressiondiagnostic assayenzyme-linked immunosorbent assay |
spellingShingle | Thulaganyo Domfe Zikhona Njengele-Tetyana Nikiwe Mhlanga Phumlani Tetyana Amanda Skepu Jane Catherine Ngila Lucky Mashudu Sikhwivhilu Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Diagnostics Rift Valley fever virus protein expression diagnostic assay enzyme-linked immunosorbent assay |
title | Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies |
title_full | Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies |
title_fullStr | Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies |
title_full_unstemmed | Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies |
title_short | Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies |
title_sort | development of a versatile half strip lateral flow assay toward the detection of rift valley fever virus antibodies |
topic | Rift Valley fever virus protein expression diagnostic assay enzyme-linked immunosorbent assay |
url | https://www.mdpi.com/2075-4418/12/11/2664 |
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