Rutin and Gallic Acid Regulates Mitochondrial Functions via the SIRT1 Pathway in C2C12 Myotubes
Mitochondria are highly dynamic organelles, balancing synthesis and degradation in response to increases in mitochondrial turnover (i.e., biogenesis, fusion, fission, and mitophagy) and function. The aim of this study was to investigate the role of polyphenols in the regulation of mitochondrial func...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/2/286 |
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| author | Wei-Tang Chang Shih-Chien Huang Hsin-Lin Cheng Shiuan-Chih Chen Chin-Lin Hsu |
| author_facet | Wei-Tang Chang Shih-Chien Huang Hsin-Lin Cheng Shiuan-Chih Chen Chin-Lin Hsu |
| author_sort | Wei-Tang Chang |
| collection | DOAJ |
| description | Mitochondria are highly dynamic organelles, balancing synthesis and degradation in response to increases in mitochondrial turnover (i.e., biogenesis, fusion, fission, and mitophagy) and function. The aim of this study was to investigate the role of polyphenols in the regulation of mitochondrial functions and dynamics in C2C12 myotubes and their molecular mechanisms. Our results indicate that gallic acid and rutin are the most potential polyphenol compounds in response to 15 phenolic acids and 5 flavonoids. Gallic acid and rutin were associated with a significantly greater mitochondrial DNA (<i>cytochrome b</i> and <i>COX-II</i>), mitochondrial enzymatic activities (including citrate synthase and cytochrome <i>c</i> oxidase), and intracellular ATP levels in C2C12 myotubes. Moreover, gallic acid and rutin significantly increased the gene expressions of mitochondrial turnover in C2C12 myotubes. Our findings indicated that gallic acid and rutin may have a beneficial effect on mitochondrial dynamics via regulation of the SIRT1-associated pathway in C2C12 myotubes. |
| first_indexed | 2024-03-09T00:54:10Z |
| format | Article |
| id | doaj.art-08b1a14a309448878ac75291572b46ca |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T00:54:10Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-08b1a14a309448878ac75291572b46ca2023-12-11T16:58:58ZengMDPI AGAntioxidants2076-39212021-02-0110228610.3390/antiox10020286Rutin and Gallic Acid Regulates Mitochondrial Functions via the SIRT1 Pathway in C2C12 MyotubesWei-Tang Chang0Shih-Chien Huang1Hsin-Lin Cheng2Shiuan-Chih Chen3Chin-Lin Hsu4Department of Nutrition and Health Nutrition, Chinese Culture University, Taipei 11114, TaiwanDepartment of Nutrition, Chung Shan Medical University, Taichung 40201, TaiwanDepartment of Nutrition, Chung Shan Medical University, Taichung 40201, TaiwanInstitute of Medicine and School of Medicine, Chung Shan Medical University, Taichung 40201, TaiwanDepartment of Nutrition, Chung Shan Medical University, Taichung 40201, TaiwanMitochondria are highly dynamic organelles, balancing synthesis and degradation in response to increases in mitochondrial turnover (i.e., biogenesis, fusion, fission, and mitophagy) and function. The aim of this study was to investigate the role of polyphenols in the regulation of mitochondrial functions and dynamics in C2C12 myotubes and their molecular mechanisms. Our results indicate that gallic acid and rutin are the most potential polyphenol compounds in response to 15 phenolic acids and 5 flavonoids. Gallic acid and rutin were associated with a significantly greater mitochondrial DNA (<i>cytochrome b</i> and <i>COX-II</i>), mitochondrial enzymatic activities (including citrate synthase and cytochrome <i>c</i> oxidase), and intracellular ATP levels in C2C12 myotubes. Moreover, gallic acid and rutin significantly increased the gene expressions of mitochondrial turnover in C2C12 myotubes. Our findings indicated that gallic acid and rutin may have a beneficial effect on mitochondrial dynamics via regulation of the SIRT1-associated pathway in C2C12 myotubes.https://www.mdpi.com/2076-3921/10/2/286C2C12 myotubesrutingallic acidSirtuin-1mitochondria |
| spellingShingle | Wei-Tang Chang Shih-Chien Huang Hsin-Lin Cheng Shiuan-Chih Chen Chin-Lin Hsu Rutin and Gallic Acid Regulates Mitochondrial Functions via the SIRT1 Pathway in C2C12 Myotubes Antioxidants C2C12 myotubes rutin gallic acid Sirtuin-1 mitochondria |
| title | Rutin and Gallic Acid Regulates Mitochondrial Functions via the SIRT1 Pathway in C2C12 Myotubes |
| title_full | Rutin and Gallic Acid Regulates Mitochondrial Functions via the SIRT1 Pathway in C2C12 Myotubes |
| title_fullStr | Rutin and Gallic Acid Regulates Mitochondrial Functions via the SIRT1 Pathway in C2C12 Myotubes |
| title_full_unstemmed | Rutin and Gallic Acid Regulates Mitochondrial Functions via the SIRT1 Pathway in C2C12 Myotubes |
| title_short | Rutin and Gallic Acid Regulates Mitochondrial Functions via the SIRT1 Pathway in C2C12 Myotubes |
| title_sort | rutin and gallic acid regulates mitochondrial functions via the sirt1 pathway in c2c12 myotubes |
| topic | C2C12 myotubes rutin gallic acid Sirtuin-1 mitochondria |
| url | https://www.mdpi.com/2076-3921/10/2/286 |
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