Diamond Nanoparticles Downregulate Expression of <i>CycD</i> and <i>CycE</i> in Glioma Cells
Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present s...
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MDPI AG
2019-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/8/1549 |
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| author | Marta Grodzik Jaroslaw Szczepaniak Barbara Strojny-Cieslak Anna Hotowy Mateusz Wierzbicki Slawomir Jaworski Marta Kutwin Emilia Soltan Tomasz Mandat Aneta Lewicka Andre Chwalibog |
| author_facet | Marta Grodzik Jaroslaw Szczepaniak Barbara Strojny-Cieslak Anna Hotowy Mateusz Wierzbicki Slawomir Jaworski Marta Kutwin Emilia Soltan Tomasz Mandat Aneta Lewicka Andre Chwalibog |
| author_sort | Marta Grodzik |
| collection | DOAJ |
| description | Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (<i>Rb, E2F1, CycA, CycB, CycD, CycE, PTEN, Ki-67</i>). After 72 h of ND treatment, the expression level of <i>Rb, CycD</i>, and <i>CycE</i> in the U118 cells, and <i>E2F1</i>, <i>CycD</i>, and <i>CycE</i> in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as <i>PTEN</i> and <i>Ki-67</i> expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells. |
| first_indexed | 2024-12-12T02:12:44Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T02:12:44Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-08b24017dfa441a995c05d6e722b8b042022-12-22T00:41:52ZengMDPI AGMolecules1420-30492019-04-01248154910.3390/molecules24081549molecules24081549Diamond Nanoparticles Downregulate Expression of <i>CycD</i> and <i>CycE</i> in Glioma CellsMarta Grodzik0Jaroslaw Szczepaniak1Barbara Strojny-Cieslak2Anna Hotowy3Mateusz Wierzbicki4Slawomir Jaworski5Marta Kutwin6Emilia Soltan7Tomasz Mandat8Aneta Lewicka9Andre Chwalibog10Division of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandDivision of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandDivision of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandDivision of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandDivision of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandDivision of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandDivision of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandDepartment of Neurosurgery, Oncology Center- Maria Sklodowska Curie Memorial, Warsaw, Roentgena 5, 02-781 Warsaw, PolandDepartment of Neurosurgery, Oncology Center- Maria Sklodowska Curie Memorial, Warsaw, Roentgena 5, 02-781 Warsaw, PolandLaboratory of Epidemiology, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, PolandDepartment of Veterinary and Animal Sciences, University of Copenhagen, Groennegaardsvej 3, 1870 Frederiksberg, DenmarkOur previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (<i>Rb, E2F1, CycA, CycB, CycD, CycE, PTEN, Ki-67</i>). After 72 h of ND treatment, the expression level of <i>Rb, CycD</i>, and <i>CycE</i> in the U118 cells, and <i>E2F1</i>, <i>CycD</i>, and <i>CycE</i> in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as <i>PTEN</i> and <i>Ki-67</i> expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells.https://www.mdpi.com/1420-3049/24/8/1549diamond nanoparticlesglioblastomaproliferationcell cyclecancer |
| spellingShingle | Marta Grodzik Jaroslaw Szczepaniak Barbara Strojny-Cieslak Anna Hotowy Mateusz Wierzbicki Slawomir Jaworski Marta Kutwin Emilia Soltan Tomasz Mandat Aneta Lewicka Andre Chwalibog Diamond Nanoparticles Downregulate Expression of <i>CycD</i> and <i>CycE</i> in Glioma Cells Molecules diamond nanoparticles glioblastoma proliferation cell cycle cancer |
| title | Diamond Nanoparticles Downregulate Expression of <i>CycD</i> and <i>CycE</i> in Glioma Cells |
| title_full | Diamond Nanoparticles Downregulate Expression of <i>CycD</i> and <i>CycE</i> in Glioma Cells |
| title_fullStr | Diamond Nanoparticles Downregulate Expression of <i>CycD</i> and <i>CycE</i> in Glioma Cells |
| title_full_unstemmed | Diamond Nanoparticles Downregulate Expression of <i>CycD</i> and <i>CycE</i> in Glioma Cells |
| title_short | Diamond Nanoparticles Downregulate Expression of <i>CycD</i> and <i>CycE</i> in Glioma Cells |
| title_sort | diamond nanoparticles downregulate expression of i cycd i and i cyce i in glioma cells |
| topic | diamond nanoparticles glioblastoma proliferation cell cycle cancer |
| url | https://www.mdpi.com/1420-3049/24/8/1549 |
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