Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation
The method of solid-state fermentation (SSF) represents a powerful technology for the fortification of animal-based by-products. Oleaginous Zygomycetes fungi are efficient microbial cell factories used in SSF to valorize a wide range of waste and rest cereal materials. The application of this fermen...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/1/170 |
| _version_ | 1797411535888318464 |
|---|---|
| author | Ondrej Slaný Tatiana Klempová Volha Shapaval Boris Zimmermann Achim Kohler Milan Čertík |
| author_facet | Ondrej Slaný Tatiana Klempová Volha Shapaval Boris Zimmermann Achim Kohler Milan Čertík |
| author_sort | Ondrej Slaný |
| collection | DOAJ |
| description | The method of solid-state fermentation (SSF) represents a powerful technology for the fortification of animal-based by-products. Oleaginous Zygomycetes fungi are efficient microbial cell factories used in SSF to valorize a wide range of waste and rest cereal materials. The application of this fermentation technique for utilization and biotransformation of animal-based materials represents a distinguished step in their treatment. In this study, for the first time, the strain <i>Umbelopsis isabellina</i> CCF2412 was used for the bioconversion of animal fat by-products to the fermented bioproducts enriched with n-6 polyunsaturated fatty acids, mainly γ-linolenic acid (GLA). Bioconversion of both cereals and the animal fat by-product resulted in the production of fermented bioproducts enriched with not just GLA (maximal yield was 6.4 mg GLA/g of fermented bioproduct), but also with high yields of glucosamine. Moreover, the fermentation on the cornmeal matrix led to obtaining bioproduct enriched with β-carotene. An increased amount of β-carotene content improved the antioxidant stability of obtained fermented bioproducts. Furthermore, the application of Fourier-transform infrared spectroscopy for rapid analysis and characterization of the biochemical profile of obtained SSF bioproducts was also studied. |
| first_indexed | 2024-03-09T04:47:30Z |
| format | Article |
| id | doaj.art-08b24747d2c84c6a9fb155f781340fce |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:47:30Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-08b24747d2c84c6a9fb155f781340fce2023-12-03T13:14:01ZengMDPI AGMicroorganisms2076-26072021-01-019117010.3390/microorganisms9010170Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State FermentationOndrej Slaný0Tatiana Klempová1Volha Shapaval2Boris Zimmermann3Achim Kohler4Milan Čertík5Faculty of Chemical and Food Technology, Institute of Biotechnology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, SlovakiaFaculty of Chemical and Food Technology, Institute of Biotechnology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, SlovakiaFaculty of Science and Technology, Norwegian University of Life Sciences, Postbox 5003, 1432 Ås, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, Postbox 5003, 1432 Ås, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, Postbox 5003, 1432 Ås, NorwayFaculty of Chemical and Food Technology, Institute of Biotechnology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, SlovakiaThe method of solid-state fermentation (SSF) represents a powerful technology for the fortification of animal-based by-products. Oleaginous Zygomycetes fungi are efficient microbial cell factories used in SSF to valorize a wide range of waste and rest cereal materials. The application of this fermentation technique for utilization and biotransformation of animal-based materials represents a distinguished step in their treatment. In this study, for the first time, the strain <i>Umbelopsis isabellina</i> CCF2412 was used for the bioconversion of animal fat by-products to the fermented bioproducts enriched with n-6 polyunsaturated fatty acids, mainly γ-linolenic acid (GLA). Bioconversion of both cereals and the animal fat by-product resulted in the production of fermented bioproducts enriched with not just GLA (maximal yield was 6.4 mg GLA/g of fermented bioproduct), but also with high yields of glucosamine. Moreover, the fermentation on the cornmeal matrix led to obtaining bioproduct enriched with β-carotene. An increased amount of β-carotene content improved the antioxidant stability of obtained fermented bioproducts. Furthermore, the application of Fourier-transform infrared spectroscopy for rapid analysis and characterization of the biochemical profile of obtained SSF bioproducts was also studied.https://www.mdpi.com/2076-2607/9/1/170solid-state fermentation<i>Umbelopsis isabellina</i>γ-linolenic acidβ-caroteneFourier transform infrared (FTIR) spectroscopyanimal fat by-product |
| spellingShingle | Ondrej Slaný Tatiana Klempová Volha Shapaval Boris Zimmermann Achim Kohler Milan Čertík Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation Microorganisms solid-state fermentation <i>Umbelopsis isabellina</i> γ-linolenic acid β-carotene Fourier transform infrared (FTIR) spectroscopy animal fat by-product |
| title | Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation |
| title_full | Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation |
| title_fullStr | Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation |
| title_full_unstemmed | Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation |
| title_short | Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation |
| title_sort | animal fat as a substrate for production of n 6 fatty acids by fungal solid state fermentation |
| topic | solid-state fermentation <i>Umbelopsis isabellina</i> γ-linolenic acid β-carotene Fourier transform infrared (FTIR) spectroscopy animal fat by-product |
| url | https://www.mdpi.com/2076-2607/9/1/170 |
| work_keys_str_mv | AT ondrejslany animalfatasasubstrateforproductionofn6fattyacidsbyfungalsolidstatefermentation AT tatianaklempova animalfatasasubstrateforproductionofn6fattyacidsbyfungalsolidstatefermentation AT volhashapaval animalfatasasubstrateforproductionofn6fattyacidsbyfungalsolidstatefermentation AT boriszimmermann animalfatasasubstrateforproductionofn6fattyacidsbyfungalsolidstatefermentation AT achimkohler animalfatasasubstrateforproductionofn6fattyacidsbyfungalsolidstatefermentation AT milancertik animalfatasasubstrateforproductionofn6fattyacidsbyfungalsolidstatefermentation |