The Cytotoxicity of RNase-Derived Peptides
Bacterial ribonuclease binase exhibits a cytotoxic effect on tumor cells possessing certain oncogenes. The aim of this study was to identify the structural parts of the binase molecule that exert cytotoxicity. Out of five designed peptides, the peptides representing the binase regions 21–50 and 74–9...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/1/16 |
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| author | Vera Ulyanova Elena Dudkina Alsu Nadyrova Vladimir Kalashnikov Yulia Surchenko Olga Ilinskaya |
| author_facet | Vera Ulyanova Elena Dudkina Alsu Nadyrova Vladimir Kalashnikov Yulia Surchenko Olga Ilinskaya |
| author_sort | Vera Ulyanova |
| collection | DOAJ |
| description | Bacterial ribonuclease binase exhibits a cytotoxic effect on tumor cells possessing certain oncogenes. The aim of this study was to identify the structural parts of the binase molecule that exert cytotoxicity. Out of five designed peptides, the peptides representing the binase regions 21–50 and 74–94 have the highest cytotoxic potential toward human cervical HeLa and breast BT-20 and MCF-7 cancer cells. The peptides B21–50 and B74–94 were not able to enter human lung adenocarcinoma A549 cells, unlike BT-20 cells, explaining their failure to inhibit A549 cell proliferation. The peptide B74–94 shares similarities with epidermal growth factor (EGF), suggesting the peptide’s specificity for EGF receptor overexpressed in BT-20 cells. Thus, the binase-derived peptides have the potential of being further developed as tumor-targeting peptides. |
| first_indexed | 2024-03-10T13:45:09Z |
| format | Article |
| id | doaj.art-08b55f79b27549869603cec7421da12c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T13:45:09Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-08b55f79b27549869603cec7421da12c2023-11-21T02:39:47ZengMDPI AGBiomolecules2218-273X2020-12-011111610.3390/biom11010016The Cytotoxicity of RNase-Derived PeptidesVera Ulyanova0Elena Dudkina1Alsu Nadyrova2Vladimir Kalashnikov3Yulia Surchenko4Olga Ilinskaya5Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaDepartment of Microbiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaDepartment of Microbiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaDepartment of Microbiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaDepartment of Microbiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaDepartment of Microbiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaBacterial ribonuclease binase exhibits a cytotoxic effect on tumor cells possessing certain oncogenes. The aim of this study was to identify the structural parts of the binase molecule that exert cytotoxicity. Out of five designed peptides, the peptides representing the binase regions 21–50 and 74–94 have the highest cytotoxic potential toward human cervical HeLa and breast BT-20 and MCF-7 cancer cells. The peptides B21–50 and B74–94 were not able to enter human lung adenocarcinoma A549 cells, unlike BT-20 cells, explaining their failure to inhibit A549 cell proliferation. The peptide B74–94 shares similarities with epidermal growth factor (EGF), suggesting the peptide’s specificity for EGF receptor overexpressed in BT-20 cells. Thus, the binase-derived peptides have the potential of being further developed as tumor-targeting peptides.https://www.mdpi.com/2218-273X/11/1/16anticancer peptidesribonuclease (RNase)binasecytotoxicityEGFepidermal growth factor (EGFR) |
| spellingShingle | Vera Ulyanova Elena Dudkina Alsu Nadyrova Vladimir Kalashnikov Yulia Surchenko Olga Ilinskaya The Cytotoxicity of RNase-Derived Peptides Biomolecules anticancer peptides ribonuclease (RNase) binase cytotoxicity EGF epidermal growth factor (EGFR) |
| title | The Cytotoxicity of RNase-Derived Peptides |
| title_full | The Cytotoxicity of RNase-Derived Peptides |
| title_fullStr | The Cytotoxicity of RNase-Derived Peptides |
| title_full_unstemmed | The Cytotoxicity of RNase-Derived Peptides |
| title_short | The Cytotoxicity of RNase-Derived Peptides |
| title_sort | cytotoxicity of rnase derived peptides |
| topic | anticancer peptides ribonuclease (RNase) binase cytotoxicity EGF epidermal growth factor (EGFR) |
| url | https://www.mdpi.com/2218-273X/11/1/16 |
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