Development of a Simple Method for Labeling and Identification of Protein Binders in Art
Easel paintings are assets with an important historic and cultural value. They usually possess a multi-tiered structure, composed of different layers some of which may present protein binders, making it important to identify these materials for restoration and conservation purposes. We propose the i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Heritage |
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| Online Access: | https://www.mdpi.com/2571-9408/2/3/150 |
| _version_ | 1811204352289800192 |
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| author | Su Yin Ooi Cátia Salvador Sergio Martins António Pereira Ana Teresa Caldeira João P Prates Ramalho |
| author_facet | Su Yin Ooi Cátia Salvador Sergio Martins António Pereira Ana Teresa Caldeira João P Prates Ramalho |
| author_sort | Su Yin Ooi |
| collection | DOAJ |
| description | Easel paintings are assets with an important historic and cultural value. They usually possess a multi-tiered structure, composed of different layers some of which may present protein binders, making it important to identify these materials for restoration and conservation purposes. We propose the identification of different protein binders by a new fluorescent labeling method employing a coumarin based chromophore, C392STP (sodium(E/Z)-4-(4-(2-(6,7-dimethoxycoumarin-3-yl)vinyl)benzoyl)-2,3,5,6-tetrafluorobenzenesulfo-nate). The method was optimized using commercial proteins and was further tested on proteins extracted from hen’s egg yolk, white bovine milk, and rabbit skin glue. To model more realistic conditions, paint models of easel paintings were prepared. The paint models were made with hen’s egg yolk, white bovine milk, and rabbit skin glue, mixed with different pigments and submitted to artificial aging. Then the extracted proteins from the paint models were labeled with C392 which allowed a sensitive and selective identification by polyacrylamide gel electrophoresis (PAGE) of the different protein binders used. As a final test, three 19th century easel paintings, from the Italian painter Giorgio Marini, were analyzed. The results show the potential of the proposed method for the identification of protein binders present in easel paintings. |
| first_indexed | 2024-04-12T03:11:40Z |
| format | Article |
| id | doaj.art-08b5dce8260843c89dd726aefea9adbc |
| institution | Directory Open Access Journal |
| issn | 2571-9408 |
| language | English |
| last_indexed | 2024-04-12T03:11:40Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Heritage |
| spelling | doaj.art-08b5dce8260843c89dd726aefea9adbc2022-12-22T03:50:19ZengMDPI AGHeritage2571-94082019-08-01232444245610.3390/heritage2030150heritage2030150Development of a Simple Method for Labeling and Identification of Protein Binders in ArtSu Yin Ooi0Cátia Salvador1Sergio Martins2António Pereira3Ana Teresa Caldeira4João P Prates Ramalho5HERCULES Laboratory, Universidade of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalHERCULES Laboratory, Universidade of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalHERCULES Laboratory, Universidade of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalHERCULES Laboratory, Universidade of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalHERCULES Laboratory, Universidade of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalChemistry Department, School of Sciences and Technology, Universidade of Évora, Rua Romão Ramalho 59, 7000-671 Évora, PortugalEasel paintings are assets with an important historic and cultural value. They usually possess a multi-tiered structure, composed of different layers some of which may present protein binders, making it important to identify these materials for restoration and conservation purposes. We propose the identification of different protein binders by a new fluorescent labeling method employing a coumarin based chromophore, C392STP (sodium(E/Z)-4-(4-(2-(6,7-dimethoxycoumarin-3-yl)vinyl)benzoyl)-2,3,5,6-tetrafluorobenzenesulfo-nate). The method was optimized using commercial proteins and was further tested on proteins extracted from hen’s egg yolk, white bovine milk, and rabbit skin glue. To model more realistic conditions, paint models of easel paintings were prepared. The paint models were made with hen’s egg yolk, white bovine milk, and rabbit skin glue, mixed with different pigments and submitted to artificial aging. Then the extracted proteins from the paint models were labeled with C392 which allowed a sensitive and selective identification by polyacrylamide gel electrophoresis (PAGE) of the different protein binders used. As a final test, three 19th century easel paintings, from the Italian painter Giorgio Marini, were analyzed. The results show the potential of the proposed method for the identification of protein binders present in easel paintings.https://www.mdpi.com/2571-9408/2/3/150fluorescent labelling methodcoumarinsprotein binderseasel paintingselectrophoretic profiling |
| spellingShingle | Su Yin Ooi Cátia Salvador Sergio Martins António Pereira Ana Teresa Caldeira João P Prates Ramalho Development of a Simple Method for Labeling and Identification of Protein Binders in Art Heritage fluorescent labelling method coumarins protein binders easel paintings electrophoretic profiling |
| title | Development of a Simple Method for Labeling and Identification of Protein Binders in Art |
| title_full | Development of a Simple Method for Labeling and Identification of Protein Binders in Art |
| title_fullStr | Development of a Simple Method for Labeling and Identification of Protein Binders in Art |
| title_full_unstemmed | Development of a Simple Method for Labeling and Identification of Protein Binders in Art |
| title_short | Development of a Simple Method for Labeling and Identification of Protein Binders in Art |
| title_sort | development of a simple method for labeling and identification of protein binders in art |
| topic | fluorescent labelling method coumarins protein binders easel paintings electrophoretic profiling |
| url | https://www.mdpi.com/2571-9408/2/3/150 |
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