Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns
Abstract Introduction HIV‐exposed uninfected (HEU) newborns suffer from higher risks of opportunistic infections during the first months of life compared to HIV‐unexposed uninfected (HUU) newborns. Alterations in thymic mass, amounts of T helper (Th) cells, T‐cell receptor diversity, and activation...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-12-01
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| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.507 |
| _version_ | 1819259691362942976 |
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| author | Yesenia Brito‐Pérez Rodrigo T. Camacho‐Pacheco Noemi Plazola‐Camacho Diana Soriano‐Becerril Irma A. Coronado‐Zarco Gabriela Arreola‐Ramírez Gabriela González‐Pérez Alma Herrera‐Salazar Julio Flores‐González Mextli Y. Bermejo‐Haro Brenda G. Casorla‐Cervantes Ismael A. Soto‐López Jessica Hernández‐Pineda Claudia Sandoval‐Montes Sandra Rodríguez‐Martínez Ricardo Figueroa‐Damian Ismael Mancilla‐Herrera |
| author_facet | Yesenia Brito‐Pérez Rodrigo T. Camacho‐Pacheco Noemi Plazola‐Camacho Diana Soriano‐Becerril Irma A. Coronado‐Zarco Gabriela Arreola‐Ramírez Gabriela González‐Pérez Alma Herrera‐Salazar Julio Flores‐González Mextli Y. Bermejo‐Haro Brenda G. Casorla‐Cervantes Ismael A. Soto‐López Jessica Hernández‐Pineda Claudia Sandoval‐Montes Sandra Rodríguez‐Martínez Ricardo Figueroa‐Damian Ismael Mancilla‐Herrera |
| author_sort | Yesenia Brito‐Pérez |
| collection | DOAJ |
| description | Abstract Introduction HIV‐exposed uninfected (HEU) newborns suffer from higher risks of opportunistic infections during the first months of life compared to HIV‐unexposed uninfected (HUU) newborns. Alterations in thymic mass, amounts of T helper (Th) cells, T‐cell receptor diversity, and activation markers have been found in HEU newborns, suggesting alterations in T cell ontogeny and differentiation. However, little is known about the ability of these cells to produce specialized Th responses from CD4+ T cells. Method To characterize the Th cell profile, we evaluated the frequency of Th1 (CD183+CD194−CD196−/CXCR3+CCR4−CCR6−), Th2 (CD183−CD194+CD196−/CXCR3−CCR4+CCR6−), Th17 (CD183−CD194+CD196+/CXCR3−CCR4+CCR6+), and CD4+CD25++ blood T‐cell phenotypes in 50 HEU and 25 HUU newborns. Early activation markers on CD4+ T cells and the Th cytokine profile produced from mononuclear cells under polyclonal T cell stimulation were also studied. Additionally, we probed the ability of CD4+ T cells to differentiate into interferon (IFN)‐γ‐producing Th1 CD4+ T cells in vitro. Results Lower percentages of differentiated Th1, Th2, Th17, and CD4+CD25++ T cells were found in blood from HEU newborns than in blood from HUU newborns. However, polyclonally stimulated Th cells showed a similar ability to express CD69 and CD279 but produced less secreted interleukin (IL)‐2 and IL‐4. Interestingly, under Th1 differentiation conditions, the percentages of CD4+IFN‐γ+ T cells and soluble IFN‐γ were higher in HEU newborns than in HUU newborns. Conclusion HEU neonates are born with reduced proportions of differentiated Th1/Th2/Th17 and CD4+CD25++ T cells, but the intrinsic abilities of CD4+ T cells to acquire a Th1 profile are not affected by the adverse maternal milieu during development. |
| first_indexed | 2024-12-23T19:14:02Z |
| format | Article |
| id | doaj.art-08b8026913fc4464bf52d9b69d5fc5c2 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-12-23T19:14:02Z |
| publishDate | 2021-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-08b8026913fc4464bf52d9b69d5fc5c22022-12-21T17:34:21ZengWileyImmunity, Inflammation and Disease2050-45272021-12-01941541155310.1002/iid3.507Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newbornsYesenia Brito‐Pérez0Rodrigo T. Camacho‐Pacheco1Noemi Plazola‐Camacho2Diana Soriano‐Becerril3Irma A. Coronado‐Zarco4Gabriela Arreola‐Ramírez5Gabriela González‐Pérez6Alma Herrera‐Salazar7Julio Flores‐González8Mextli Y. Bermejo‐Haro9Brenda G. Casorla‐Cervantes10Ismael A. Soto‐López11Jessica Hernández‐Pineda12Claudia Sandoval‐Montes13Sandra Rodríguez‐Martínez14Ricardo Figueroa‐Damian15Ismael Mancilla‐Herrera16Infectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoNeonatology Department National Institute of Perinatology (INPer) Mexico City MexicoNeonatology Department National Institute of Perinatology (INPer) Mexico City MexicoDepartment of Physiology and Cellular Development National Institute of Perinatology (INPer) Mexico City MexicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoDepartamento de Inmunología, Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional Ciudad de México MéxicoDepartamento de Inmunología, Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional Ciudad de México MéxicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoInfectology and Immunology Department National Institute of Perinatology (INPer) Mexico City MexicoAbstract Introduction HIV‐exposed uninfected (HEU) newborns suffer from higher risks of opportunistic infections during the first months of life compared to HIV‐unexposed uninfected (HUU) newborns. Alterations in thymic mass, amounts of T helper (Th) cells, T‐cell receptor diversity, and activation markers have been found in HEU newborns, suggesting alterations in T cell ontogeny and differentiation. However, little is known about the ability of these cells to produce specialized Th responses from CD4+ T cells. Method To characterize the Th cell profile, we evaluated the frequency of Th1 (CD183+CD194−CD196−/CXCR3+CCR4−CCR6−), Th2 (CD183−CD194+CD196−/CXCR3−CCR4+CCR6−), Th17 (CD183−CD194+CD196+/CXCR3−CCR4+CCR6+), and CD4+CD25++ blood T‐cell phenotypes in 50 HEU and 25 HUU newborns. Early activation markers on CD4+ T cells and the Th cytokine profile produced from mononuclear cells under polyclonal T cell stimulation were also studied. Additionally, we probed the ability of CD4+ T cells to differentiate into interferon (IFN)‐γ‐producing Th1 CD4+ T cells in vitro. Results Lower percentages of differentiated Th1, Th2, Th17, and CD4+CD25++ T cells were found in blood from HEU newborns than in blood from HUU newborns. However, polyclonally stimulated Th cells showed a similar ability to express CD69 and CD279 but produced less secreted interleukin (IL)‐2 and IL‐4. Interestingly, under Th1 differentiation conditions, the percentages of CD4+IFN‐γ+ T cells and soluble IFN‐γ were higher in HEU newborns than in HUU newborns. Conclusion HEU neonates are born with reduced proportions of differentiated Th1/Th2/Th17 and CD4+CD25++ T cells, but the intrinsic abilities of CD4+ T cells to acquire a Th1 profile are not affected by the adverse maternal milieu during development.https://doi.org/10.1002/iid3.507HIV‐exposed uninfected newbornsTh cellsTh1/Th2/Th17/Treg/CD4+CD25++ T cells and Th1 differentiation |
| spellingShingle | Yesenia Brito‐Pérez Rodrigo T. Camacho‐Pacheco Noemi Plazola‐Camacho Diana Soriano‐Becerril Irma A. Coronado‐Zarco Gabriela Arreola‐Ramírez Gabriela González‐Pérez Alma Herrera‐Salazar Julio Flores‐González Mextli Y. Bermejo‐Haro Brenda G. Casorla‐Cervantes Ismael A. Soto‐López Jessica Hernández‐Pineda Claudia Sandoval‐Montes Sandra Rodríguez‐Martínez Ricardo Figueroa‐Damian Ismael Mancilla‐Herrera Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns Immunity, Inflammation and Disease HIV‐exposed uninfected newborns Th cells Th1/Th2/Th17/Treg/CD4+CD25++ T cells and Th1 differentiation |
| title | Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns |
| title_full | Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns |
| title_fullStr | Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns |
| title_full_unstemmed | Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns |
| title_short | Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns |
| title_sort | impaired t helper cell responses in human immunodeficiency virus exposed uninfected newborns |
| topic | HIV‐exposed uninfected newborns Th cells Th1/Th2/Th17/Treg/CD4+CD25++ T cells and Th1 differentiation |
| url | https://doi.org/10.1002/iid3.507 |
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