The Role of Nod Factor Substituents in Actin Cytoskeleton Rearrangements in Phaseolus vulgaris
In order to define the symbiotic role of some of the chemical substituents in the Rhizobium etli Nod factors (NFs), we purified Nod metabolites secreted by the SM25 strain, which carries most of the nodulation genes, and SM17 with an insertion in nodS. These NFs were analyzed for their capabilities...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2003-04-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.4.326 |
| _version_ | 1811264556295520256 |
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| author | Luis Cárdenas Jane E. Thomas-Oates Noreide Nava Isabel M. López-Lara Peter K. Hepler Carmen Quinto |
| author_facet | Luis Cárdenas Jane E. Thomas-Oates Noreide Nava Isabel M. López-Lara Peter K. Hepler Carmen Quinto |
| author_sort | Luis Cárdenas |
| collection | DOAJ |
| description | In order to define the symbiotic role of some of the chemical substituents in the Rhizobium etli Nod factors (NFs), we purified Nod metabolites secreted by the SM25 strain, which carries most of the nodulation genes, and SM17 with an insertion in nodS. These NFs were analyzed for their capabilities to induce root hair curling and cytoskeletal rearrangements. The NFs secreted by strain SM17 lack the carbamoyl and methyl substituents on the nonreducing terminal residue and an acetyl moiety on the fucosyl residue on the reducing-terminal residue as determined by mass spectrometry. We have reported previously that the root hair cell actin cytoskeleton from bean responds with a rapid fragmentation of the actin bundles within 5 min of NF exposure, and also is accompanied by increases in the apical influxes and intracellular calcium levels. In this article, we report that methyl-bearing NFs are more active in inducing root hair curling and actin cytoskeleton rearrangements than nonmethylated NFs. However, the carbamoyl residue on the nonreducing terminal residue and the acetyl group at the fucosyl residue on the reducing terminal residue do not seem to have any effect on root hair curling induction or in actin cytoskeleton rearrangement. |
| first_indexed | 2024-04-12T20:05:16Z |
| format | Article |
| id | doaj.art-08b851c7321e4a32a1a98bc05e5b2714 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:05:16Z |
| publishDate | 2003-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-08b851c7321e4a32a1a98bc05e5b27142022-12-22T03:18:24ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062003-04-0116432633410.1094/MPMI.2003.16.4.326The Role of Nod Factor Substituents in Actin Cytoskeleton Rearrangements in Phaseolus vulgarisLuis CárdenasJane E. Thomas-OatesNoreide NavaIsabel M. López-LaraPeter K. HeplerCarmen QuintoIn order to define the symbiotic role of some of the chemical substituents in the Rhizobium etli Nod factors (NFs), we purified Nod metabolites secreted by the SM25 strain, which carries most of the nodulation genes, and SM17 with an insertion in nodS. These NFs were analyzed for their capabilities to induce root hair curling and cytoskeletal rearrangements. The NFs secreted by strain SM17 lack the carbamoyl and methyl substituents on the nonreducing terminal residue and an acetyl moiety on the fucosyl residue on the reducing-terminal residue as determined by mass spectrometry. We have reported previously that the root hair cell actin cytoskeleton from bean responds with a rapid fragmentation of the actin bundles within 5 min of NF exposure, and also is accompanied by increases in the apical influxes and intracellular calcium levels. In this article, we report that methyl-bearing NFs are more active in inducing root hair curling and actin cytoskeleton rearrangements than nonmethylated NFs. However, the carbamoyl residue on the nonreducing terminal residue and the acetyl group at the fucosyl residue on the reducing terminal residue do not seem to have any effect on root hair curling induction or in actin cytoskeleton rearrangement.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.4.326FITC-phalloidinlipochitin-oligosaccharidemicroinjection |
| spellingShingle | Luis Cárdenas Jane E. Thomas-Oates Noreide Nava Isabel M. López-Lara Peter K. Hepler Carmen Quinto The Role of Nod Factor Substituents in Actin Cytoskeleton Rearrangements in Phaseolus vulgaris Molecular Plant-Microbe Interactions FITC-phalloidin lipochitin-oligosaccharide microinjection |
| title | The Role of Nod Factor Substituents in Actin Cytoskeleton Rearrangements in Phaseolus vulgaris |
| title_full | The Role of Nod Factor Substituents in Actin Cytoskeleton Rearrangements in Phaseolus vulgaris |
| title_fullStr | The Role of Nod Factor Substituents in Actin Cytoskeleton Rearrangements in Phaseolus vulgaris |
| title_full_unstemmed | The Role of Nod Factor Substituents in Actin Cytoskeleton Rearrangements in Phaseolus vulgaris |
| title_short | The Role of Nod Factor Substituents in Actin Cytoskeleton Rearrangements in Phaseolus vulgaris |
| title_sort | role of nod factor substituents in actin cytoskeleton rearrangements in phaseolus vulgaris |
| topic | FITC-phalloidin lipochitin-oligosaccharide microinjection |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.4.326 |
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