Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers.
Understanding of genetic diversity is important to explore existing gene in any crop breeding program. Most of the diversity preserved in the landraces which are well-known reservoirs of important traits for biotic and abiotic stresses. In the present study, the genetic diversity at twenty-four most...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0211061 |
| _version_ | 1819114950881181696 |
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| author | Manoj Kumar Yadav S Aravindan Umakanta Ngangkham S Raghu S R Prabhukarthikeyan U Keerthana B C Marndi Totan Adak Susmita Munda Rupesh Deshmukh D Pramesh Sanghamitra Samantaray P C Rath |
| author_facet | Manoj Kumar Yadav S Aravindan Umakanta Ngangkham S Raghu S R Prabhukarthikeyan U Keerthana B C Marndi Totan Adak Susmita Munda Rupesh Deshmukh D Pramesh Sanghamitra Samantaray P C Rath |
| author_sort | Manoj Kumar Yadav |
| collection | DOAJ |
| description | Understanding of genetic diversity is important to explore existing gene in any crop breeding program. Most of the diversity preserved in the landraces which are well-known reservoirs of important traits for biotic and abiotic stresses. In the present study, the genetic diversity at twenty-four most significant blast resistance gene loci using twenty-eight gene specific markers were investigated in landraces originated from nine diverse rice ecologies of India. Based on phenotypic evaluation, landraces were classified into three distinct groups: highly resistant (21), moderately resistant (70) and susceptible (70). The landraces harbour a range of five to nineteen genes representing blast resistance allele with the frequency varied from 4.96% to 100%. The cluster analysis grouped entire 161 landraces into two major groups. Population structure along with other parameters was also analyzed to understand the evolution of blast resistance gene in rice. The population structure analysis and principal coordinate analysis classified the landraces into two sub-populations. Analysis of molecular variance showed maximum (93%) diversity within the population and least (7%) between populations. Five markers viz; K3957, Pikh, Pi2-i, RM212and RM302 were strongly associated with blast disease with the phenotypic variance of 1.4% to 7.6%. These resistant landraces will serve as a valuable genetic resource for future genomic studies, host-pathogen interaction, identification of novel R genes and rice improvement strategies. |
| first_indexed | 2024-12-22T04:53:27Z |
| format | Article |
| id | doaj.art-08bab673c82a4f1eb3f50be198f2306c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T04:53:27Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-08bab673c82a4f1eb3f50be198f2306c2022-12-21T18:38:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01141e021106110.1371/journal.pone.0211061Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers.Manoj Kumar YadavS AravindanUmakanta NgangkhamS RaghuS R PrabhukarthikeyanU KeerthanaB C MarndiTotan AdakSusmita MundaRupesh DeshmukhD PrameshSanghamitra SamantarayP C RathUnderstanding of genetic diversity is important to explore existing gene in any crop breeding program. Most of the diversity preserved in the landraces which are well-known reservoirs of important traits for biotic and abiotic stresses. In the present study, the genetic diversity at twenty-four most significant blast resistance gene loci using twenty-eight gene specific markers were investigated in landraces originated from nine diverse rice ecologies of India. Based on phenotypic evaluation, landraces were classified into three distinct groups: highly resistant (21), moderately resistant (70) and susceptible (70). The landraces harbour a range of five to nineteen genes representing blast resistance allele with the frequency varied from 4.96% to 100%. The cluster analysis grouped entire 161 landraces into two major groups. Population structure along with other parameters was also analyzed to understand the evolution of blast resistance gene in rice. The population structure analysis and principal coordinate analysis classified the landraces into two sub-populations. Analysis of molecular variance showed maximum (93%) diversity within the population and least (7%) between populations. Five markers viz; K3957, Pikh, Pi2-i, RM212and RM302 were strongly associated with blast disease with the phenotypic variance of 1.4% to 7.6%. These resistant landraces will serve as a valuable genetic resource for future genomic studies, host-pathogen interaction, identification of novel R genes and rice improvement strategies.https://doi.org/10.1371/journal.pone.0211061 |
| spellingShingle | Manoj Kumar Yadav S Aravindan Umakanta Ngangkham S Raghu S R Prabhukarthikeyan U Keerthana B C Marndi Totan Adak Susmita Munda Rupesh Deshmukh D Pramesh Sanghamitra Samantaray P C Rath Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers. PLoS ONE |
| title | Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers. |
| title_full | Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers. |
| title_fullStr | Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers. |
| title_full_unstemmed | Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers. |
| title_short | Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers. |
| title_sort | blast resistance in indian rice landraces genetic dissection by gene specific markers |
| url | https://doi.org/10.1371/journal.pone.0211061 |
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