Identification and Expression Profile of Olfactory Receptor Genes Based on Apriona germari (Hope) Antennal Transcriptome
Insects’ olfactory receptor plays a central role in detecting chemosensory information from the environment. Odorant receptors (ORs) and ionotropic receptors (IRs) are two types of olfactory receptors, and they are essential for the recognition of ligands at peripheral neurons. Apriona germari (Hope...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00807/full |
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| author | Jia-Li Qian Ding-Ze Mang Guo-Chang Lv Jia Ye Zhao-Qun Li Bo Chu Long Sun Yu-Jun Liu Long-Wa Zhang |
| author_facet | Jia-Li Qian Ding-Ze Mang Guo-Chang Lv Jia Ye Zhao-Qun Li Bo Chu Long Sun Yu-Jun Liu Long-Wa Zhang |
| author_sort | Jia-Li Qian |
| collection | DOAJ |
| description | Insects’ olfactory receptor plays a central role in detecting chemosensory information from the environment. Odorant receptors (ORs) and ionotropic receptors (IRs) are two types of olfactory receptors, and they are essential for the recognition of ligands at peripheral neurons. Apriona germari (Hope) (Coleoptera: Cerambycidae) is one of the most serious insect pests that cause damage to economic trees and landscaping trees, resulting in massive environmental damages and economic losses. Olfactory-based management strategy has been suggested as a promising strategy to control this wood-boring beetle. However, the olfactory perception mechanism in A. germari is now almost unknown. In the present study, RNA sequencing analysis was used to determine the transcriptomes of adult A. germari antennae. Among 36,834 unigenes derived from the antennal assembly, we identified 42 AgerORs and three AgerIRs. Based on the tissue expression pattern analysis, 27 AgerORs displayed a female-biased expression. Notably, AgerOR3, 5, 13, 33, and 40 showed a significant female-biased expression and were clustered with the pheromone receptors of Megacyllene caryae in the phylogenetic tree, suggesting that these AgerORs could be potential pheromone receptors for sensing male-produced sex pheromones in A. germari. The AgerIRs expression profile demonstrated that AgerIR2 had high expression levels in male labial palps, suggesting that this receptor may function to detect female-deposited trail-sex pheromone blend of A. germari. In addition, the phylogenetic tree showed that the Orco gene of five cerambycidae species was highly conservative. These results provide a foundation for further studies on the molecular mechanisms of olfactory chemoreception in A. germari apart from suggesting novel targets for the control of this pest in the future. |
| first_indexed | 2024-12-12T14:30:50Z |
| format | Article |
| id | doaj.art-08bb95aa4a0a42b39004904556aeea36 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-12T14:30:50Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-08bb95aa4a0a42b39004904556aeea362022-12-22T00:21:31ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-07-011110.3389/fphys.2020.00807544090Identification and Expression Profile of Olfactory Receptor Genes Based on Apriona germari (Hope) Antennal TranscriptomeJia-Li Qian0Ding-Ze Mang1Guo-Chang Lv2Jia Ye3Zhao-Qun Li4Bo Chu5Long Sun6Yu-Jun Liu7Long-Wa Zhang8Anhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, ChinaGraduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Tokyo, JapanAnhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, ChinaAnhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, ChinaAnhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, ChinaAnhui Academy of Science and Technology, Hefei, ChinaAnhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, ChinaInsects’ olfactory receptor plays a central role in detecting chemosensory information from the environment. Odorant receptors (ORs) and ionotropic receptors (IRs) are two types of olfactory receptors, and they are essential for the recognition of ligands at peripheral neurons. Apriona germari (Hope) (Coleoptera: Cerambycidae) is one of the most serious insect pests that cause damage to economic trees and landscaping trees, resulting in massive environmental damages and economic losses. Olfactory-based management strategy has been suggested as a promising strategy to control this wood-boring beetle. However, the olfactory perception mechanism in A. germari is now almost unknown. In the present study, RNA sequencing analysis was used to determine the transcriptomes of adult A. germari antennae. Among 36,834 unigenes derived from the antennal assembly, we identified 42 AgerORs and three AgerIRs. Based on the tissue expression pattern analysis, 27 AgerORs displayed a female-biased expression. Notably, AgerOR3, 5, 13, 33, and 40 showed a significant female-biased expression and were clustered with the pheromone receptors of Megacyllene caryae in the phylogenetic tree, suggesting that these AgerORs could be potential pheromone receptors for sensing male-produced sex pheromones in A. germari. The AgerIRs expression profile demonstrated that AgerIR2 had high expression levels in male labial palps, suggesting that this receptor may function to detect female-deposited trail-sex pheromone blend of A. germari. In addition, the phylogenetic tree showed that the Orco gene of five cerambycidae species was highly conservative. These results provide a foundation for further studies on the molecular mechanisms of olfactory chemoreception in A. germari apart from suggesting novel targets for the control of this pest in the future.https://www.frontiersin.org/article/10.3389/fphys.2020.00807/fullodorant receptorionotropic receptorexpression patternantennal transcriptomeApriona germari |
| spellingShingle | Jia-Li Qian Ding-Ze Mang Guo-Chang Lv Jia Ye Zhao-Qun Li Bo Chu Long Sun Yu-Jun Liu Long-Wa Zhang Identification and Expression Profile of Olfactory Receptor Genes Based on Apriona germari (Hope) Antennal Transcriptome Frontiers in Physiology odorant receptor ionotropic receptor expression pattern antennal transcriptome Apriona germari |
| title | Identification and Expression Profile of Olfactory Receptor Genes Based on Apriona germari (Hope) Antennal Transcriptome |
| title_full | Identification and Expression Profile of Olfactory Receptor Genes Based on Apriona germari (Hope) Antennal Transcriptome |
| title_fullStr | Identification and Expression Profile of Olfactory Receptor Genes Based on Apriona germari (Hope) Antennal Transcriptome |
| title_full_unstemmed | Identification and Expression Profile of Olfactory Receptor Genes Based on Apriona germari (Hope) Antennal Transcriptome |
| title_short | Identification and Expression Profile of Olfactory Receptor Genes Based on Apriona germari (Hope) Antennal Transcriptome |
| title_sort | identification and expression profile of olfactory receptor genes based on apriona germari hope antennal transcriptome |
| topic | odorant receptor ionotropic receptor expression pattern antennal transcriptome Apriona germari |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00807/full |
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