Comparison of area under the curve in various models of diabetic rats receiving chronic medication
Introduction The oral glucose tolerance test (OGTT) is widely used as a diagnostic tool for impaired glucose tolerance (IGT) in clinical settings and animal experiments. The area under the curve (AUC) is then developed to quantify the total increase in blood glucose during the OGTT. Similarly, atten...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Termedia Publishing House
2020-01-01
|
| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/Comparison-of-area-under-the-curve-in-various-models-of-diabetic-rats-receiving-chronic,113624,0,2.html |
| _version_ | 1797435292735504384 |
|---|---|
| author | Keng-Fan Liu Chiang-Shan Niu Jen-Chieh Tsai Chao-Lin Yang Wen-Huang Peng Ho-Shan Niu |
| author_facet | Keng-Fan Liu Chiang-Shan Niu Jen-Chieh Tsai Chao-Lin Yang Wen-Huang Peng Ho-Shan Niu |
| author_sort | Keng-Fan Liu |
| collection | DOAJ |
| description | Introduction
The oral glucose tolerance test (OGTT) is widely used as a diagnostic tool for impaired glucose tolerance (IGT) in clinical settings and animal experiments. The area under the curve (AUC) is then developed to quantify the total increase in blood glucose during the OGTT. Similarly, attenuation of the increased AUC indicates the improvement of IGT in animals. Variations in fasting plasma glucose between individuals stimulate the development of incremental area under the curve (iAUC). However, the iAUC determined from subtracting the baseline value of fasting plasma glucose (similar to ΔAUC) has been challenged as problematic without evidence.
Material and methods
We developed four different diabetic animal models. In each model, rats were treated with metformin, dapagliflozin, and insulin respectively for one week. OGTTs were performed after 7 days of the drug treatment. The acute blood glucose changes induced by one-time treatment of drugs were also compared.
Results
After a daily application of each drug at an effective dose for 7 days, results indicated potency in the following order: insulin > dapagliflozin > metformin. This was determined by calculation using the AUC in all diabetic models. However, the order changed when using the calculation with iAUC. Additionally, signals were changed before the OGTT in each model that received repeated treatment of each drug. Notably, drug potency was shown to be the same in OGTT calculated from iAUC and AUC in diabetic rats receiving acute treatment.
Conclusions
iAUC seems unsuitable for application in cases where subjects are receiving chronic medication(s). |
| first_indexed | 2024-03-09T10:46:04Z |
| format | Article |
| id | doaj.art-08bc86a1f55940a780deb3dc2a4d2d2f |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-03-09T10:46:04Z |
| publishDate | 2020-01-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-08bc86a1f55940a780deb3dc2a4d2d2f2023-12-01T10:02:33ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512020-01-011841078108710.5114/aoms.2019.91471113624Comparison of area under the curve in various models of diabetic rats receiving chronic medicationKeng-Fan Liu0Chiang-Shan Niu1Jen-Chieh Tsai2Chao-Lin Yang3Wen-Huang Peng4Ho-Shan Niu5School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, TaiwanDepartment of Nursing, Tzu Chi University of Science and Technology, Hualien, TaiwanDepartment of Medicinal Botanicals and Health Applications, Da-Yeh University, Chunghua, TaiwanCollege of Biopharmaceutical and Food Sciences, China Medical University, Taichung, TaiwanSchool of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, TaiwanDepartment of Nursing, Tzu Chi University of Science and Technology, Hualien, TaiwanIntroduction The oral glucose tolerance test (OGTT) is widely used as a diagnostic tool for impaired glucose tolerance (IGT) in clinical settings and animal experiments. The area under the curve (AUC) is then developed to quantify the total increase in blood glucose during the OGTT. Similarly, attenuation of the increased AUC indicates the improvement of IGT in animals. Variations in fasting plasma glucose between individuals stimulate the development of incremental area under the curve (iAUC). However, the iAUC determined from subtracting the baseline value of fasting plasma glucose (similar to ΔAUC) has been challenged as problematic without evidence. Material and methods We developed four different diabetic animal models. In each model, rats were treated with metformin, dapagliflozin, and insulin respectively for one week. OGTTs were performed after 7 days of the drug treatment. The acute blood glucose changes induced by one-time treatment of drugs were also compared. Results After a daily application of each drug at an effective dose for 7 days, results indicated potency in the following order: insulin > dapagliflozin > metformin. This was determined by calculation using the AUC in all diabetic models. However, the order changed when using the calculation with iAUC. Additionally, signals were changed before the OGTT in each model that received repeated treatment of each drug. Notably, drug potency was shown to be the same in OGTT calculated from iAUC and AUC in diabetic rats receiving acute treatment. Conclusions iAUC seems unsuitable for application in cases where subjects are receiving chronic medication(s).https://www.archivesofmedicalscience.com/Comparison-of-area-under-the-curve-in-various-models-of-diabetic-rats-receiving-chronic,113624,0,2.htmlimpaired glucose toleranceoral glucose tolerance testarea under the curveincremental area under curvediabetic rats |
| spellingShingle | Keng-Fan Liu Chiang-Shan Niu Jen-Chieh Tsai Chao-Lin Yang Wen-Huang Peng Ho-Shan Niu Comparison of area under the curve in various models of diabetic rats receiving chronic medication Archives of Medical Science impaired glucose tolerance oral glucose tolerance test area under the curve incremental area under curve diabetic rats |
| title | Comparison of area under the curve in various models of diabetic rats receiving chronic medication |
| title_full | Comparison of area under the curve in various models of diabetic rats receiving chronic medication |
| title_fullStr | Comparison of area under the curve in various models of diabetic rats receiving chronic medication |
| title_full_unstemmed | Comparison of area under the curve in various models of diabetic rats receiving chronic medication |
| title_short | Comparison of area under the curve in various models of diabetic rats receiving chronic medication |
| title_sort | comparison of area under the curve in various models of diabetic rats receiving chronic medication |
| topic | impaired glucose tolerance oral glucose tolerance test area under the curve incremental area under curve diabetic rats |
| url | https://www.archivesofmedicalscience.com/Comparison-of-area-under-the-curve-in-various-models-of-diabetic-rats-receiving-chronic,113624,0,2.html |
| work_keys_str_mv | AT kengfanliu comparisonofareaunderthecurveinvariousmodelsofdiabeticratsreceivingchronicmedication AT chiangshanniu comparisonofareaunderthecurveinvariousmodelsofdiabeticratsreceivingchronicmedication AT jenchiehtsai comparisonofareaunderthecurveinvariousmodelsofdiabeticratsreceivingchronicmedication AT chaolinyang comparisonofareaunderthecurveinvariousmodelsofdiabeticratsreceivingchronicmedication AT wenhuangpeng comparisonofareaunderthecurveinvariousmodelsofdiabeticratsreceivingchronicmedication AT hoshanniu comparisonofareaunderthecurveinvariousmodelsofdiabeticratsreceivingchronicmedication |