Establishment of a mouse model of atopic dermatitis with overgrowth Malassezia globosa
Objective: To establish a mouse model of atopic dermatitis (AD) with overgrowth Malassezia globosa. Methods: Eighteen BALB/c mice were randomly divided into the control group, AD group, NS/M group, AD+NS/M group, Oil/M group and AD+Oil/M group, 3 mice per group. AD model of BALB/c mice were establis...
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| Format: | Article |
| Language: | zho |
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editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology
2020-04-01
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| Series: | Pifu-xingbing zhenliaoxue zazhi |
| Subjects: | |
| Online Access: | http://pfxbzlx.gdvdc.com/CN/abstract/abstract1801.shtml |
| _version_ | 1797990419361955840 |
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| author | Xin ZHOU Pei-ying FENG Jia-min CHEN Zhuang-gui CHEN Qin-tai YANG Li-jie PAN Huai-qiu HUANG Rong-sheng SU |
| author_facet | Xin ZHOU Pei-ying FENG Jia-min CHEN Zhuang-gui CHEN Qin-tai YANG Li-jie PAN Huai-qiu HUANG Rong-sheng SU |
| author_sort | Xin ZHOU |
| collection | DOAJ |
| description | Objective: To establish a mouse model of atopic dermatitis (AD) with overgrowth Malassezia globosa. Methods: Eighteen BALB/c mice were randomly divided into the control group, AD group, NS/M group, AD+NS/M group, Oil/M group and AD+Oil/M group, 3 mice per group. AD model of BALB/c mice were established by topical administration of MC903. Fungi overgrowth experimental models were attempted to establish using suspension of M. globosa with olive oil and normal saline as vehicle, respectively. Clinical manifestations and lesion scoring as well as mycological and histopathological changes of each group were evaluated. Scanning and transmission electron microscopy were also used to observe the changes on hair follicle and hair shaft. Results: Compared with AD group, more severe AD-like lesion, obvious hair loss andincreasedlesion scoring (q=5.95, P<0.01) were observed in AD+Oil/M group. Histopathological results showed that the epidermis thickness of AD+Oil/M group was significantly increased, the dermis was infiltrated by more dense inflammatory cells, and M. globosa colonization lead to damaged hair folliclesand the degeneratedhair shafts. The results of PAS and fungal fluorescence staining showed a mass of M. globosa in the stratum corneum of ear tissues. Overgrowth of M. globosa inside and outside the hair shaft were proved by scanning and transmission electron microscopy. In NS/M group and AD+NS/M group, only a few M. globose yeast cells were observed in the ear tissue using PAS and fungal fluorescence staining, respectively. Conclusion: A mouse model of AD with overgrowth M. globosa could be established treating with suspension of M. globosa using olive oil but not normal saline. |
| first_indexed | 2024-04-11T08:36:13Z |
| format | Article |
| id | doaj.art-08bdd3a8e7ce4d668b7c87d46ba76982 |
| institution | Directory Open Access Journal |
| issn | 1674-8468 |
| language | zho |
| last_indexed | 2024-04-11T08:36:13Z |
| publishDate | 2020-04-01 |
| publisher | editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology |
| record_format | Article |
| series | Pifu-xingbing zhenliaoxue zazhi |
| spelling | doaj.art-08bdd3a8e7ce4d668b7c87d46ba769822022-12-22T04:34:19Zzhoeditoiral office of Journal of Diagnosis and Therapy on Dermato-venereologyPifu-xingbing zhenliaoxue zazhi1674-84682020-04-01272727810.3969/j.issn.1674-8468.2020.02.002Establishment of a mouse model of atopic dermatitis with overgrowth Malassezia globosaXin ZHOU0Pei-ying FENG1Jia-min CHEN2Zhuang-gui CHEN3Qin-tai YANG4Li-jie PAN5Huai-qiu HUANG6Rong-sheng SU7The Third Affiliated Hospital, Sun Yat-sen University Department of Dermatology, Guangzhou 510630, ChinaThe Third Affiliated Hospital, Sun Yat-sen University Department of Dermatology, Guangzhou 510630, ChinaThe Third Affiliated Hospital, Sun Yat-sen University Department of Dermatology, Guangzhou 510630, ChinaThe Third Affiliated Hospital, Sun Yat-sen University Department of Allergy, Guangzhou 510630, ChinaThe Third Affiliated Hospital, Sun Yat-sen University Department of Allergy, Guangzhou 510630, ChinaThe Third Affiliated Hospital, Sun Yat-sen University Vaccine Research Institute, Guangzhou 510630, ChinaThe Third Affiliated Hospital, Sun Yat-sen University Department of Dermatology, Guangzhou 510630, ChinaCollege of Veterinary Medicine South China Agricultural University, Guangzhou 510642, ChinaObjective: To establish a mouse model of atopic dermatitis (AD) with overgrowth Malassezia globosa. Methods: Eighteen BALB/c mice were randomly divided into the control group, AD group, NS/M group, AD+NS/M group, Oil/M group and AD+Oil/M group, 3 mice per group. AD model of BALB/c mice were established by topical administration of MC903. Fungi overgrowth experimental models were attempted to establish using suspension of M. globosa with olive oil and normal saline as vehicle, respectively. Clinical manifestations and lesion scoring as well as mycological and histopathological changes of each group were evaluated. Scanning and transmission electron microscopy were also used to observe the changes on hair follicle and hair shaft. Results: Compared with AD group, more severe AD-like lesion, obvious hair loss andincreasedlesion scoring (q=5.95, P<0.01) were observed in AD+Oil/M group. Histopathological results showed that the epidermis thickness of AD+Oil/M group was significantly increased, the dermis was infiltrated by more dense inflammatory cells, and M. globosa colonization lead to damaged hair folliclesand the degeneratedhair shafts. The results of PAS and fungal fluorescence staining showed a mass of M. globosa in the stratum corneum of ear tissues. Overgrowth of M. globosa inside and outside the hair shaft were proved by scanning and transmission electron microscopy. In NS/M group and AD+NS/M group, only a few M. globose yeast cells were observed in the ear tissue using PAS and fungal fluorescence staining, respectively. Conclusion: A mouse model of AD with overgrowth M. globosa could be established treating with suspension of M. globosa using olive oil but not normal saline.http://pfxbzlx.gdvdc.com/CN/abstract/abstract1801.shtmlmalassezia globosaatopic dermatitismouse modelolive oil |
| spellingShingle | Xin ZHOU Pei-ying FENG Jia-min CHEN Zhuang-gui CHEN Qin-tai YANG Li-jie PAN Huai-qiu HUANG Rong-sheng SU Establishment of a mouse model of atopic dermatitis with overgrowth Malassezia globosa Pifu-xingbing zhenliaoxue zazhi malassezia globosa atopic dermatitis mouse model olive oil |
| title | Establishment of a mouse model of atopic dermatitis with overgrowth Malassezia globosa |
| title_full | Establishment of a mouse model of atopic dermatitis with overgrowth Malassezia globosa |
| title_fullStr | Establishment of a mouse model of atopic dermatitis with overgrowth Malassezia globosa |
| title_full_unstemmed | Establishment of a mouse model of atopic dermatitis with overgrowth Malassezia globosa |
| title_short | Establishment of a mouse model of atopic dermatitis with overgrowth Malassezia globosa |
| title_sort | establishment of a mouse model of atopic dermatitis with overgrowth malassezia globosa |
| topic | malassezia globosa atopic dermatitis mouse model olive oil |
| url | http://pfxbzlx.gdvdc.com/CN/abstract/abstract1801.shtml |
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