Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2
Nucleic acid testing (NAT) played a crucial role in containing the spread of SARS-CoV-2 during the epidemic. The gold standard technique, the quantitative real-time polymerase chain reaction (qRT-PCR) technique, is currently used by the government and medical boards to detect SARS-CoV-2. Due to the...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/4/636 |
| _version_ | 1797409993800024064 |
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| author | Weidu Song Taiyi Zhang Huichao Lin Yujing Yang Gaozhen Zhao Xiaowen Huang |
| author_facet | Weidu Song Taiyi Zhang Huichao Lin Yujing Yang Gaozhen Zhao Xiaowen Huang |
| author_sort | Weidu Song |
| collection | DOAJ |
| description | Nucleic acid testing (NAT) played a crucial role in containing the spread of SARS-CoV-2 during the epidemic. The gold standard technique, the quantitative real-time polymerase chain reaction (qRT-PCR) technique, is currently used by the government and medical boards to detect SARS-CoV-2. Due to the limitations of this technology, it is not capable of meeting the needs of large-scale rapid detection. To solve this problem, many new techniques for detecting nucleic acids of SARS-CoV-2 have been reported. Therefore, a review that systematically and comprehensively introduces and compares various detection technologies is needed. In this paper, we not only review the traditional NAT but also provide an overview of microfluidic-based NAT technologies and summarize and discuss the characteristics and development prospects of these techniques. |
| first_indexed | 2024-03-09T04:22:33Z |
| format | Article |
| id | doaj.art-08be001cb2384895a6cb097023900764 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T04:22:33Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-08be001cb2384895a6cb0970239007642023-12-03T13:44:29ZengMDPI AGMicromachines2072-666X2022-04-0113463610.3390/mi13040636Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2Weidu Song0Taiyi Zhang1Huichao Lin2Yujing Yang3Gaozhen Zhao4Xiaowen Huang5State Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250300, ChinaNucleic acid testing (NAT) played a crucial role in containing the spread of SARS-CoV-2 during the epidemic. The gold standard technique, the quantitative real-time polymerase chain reaction (qRT-PCR) technique, is currently used by the government and medical boards to detect SARS-CoV-2. Due to the limitations of this technology, it is not capable of meeting the needs of large-scale rapid detection. To solve this problem, many new techniques for detecting nucleic acids of SARS-CoV-2 have been reported. Therefore, a review that systematically and comprehensively introduces and compares various detection technologies is needed. In this paper, we not only review the traditional NAT but also provide an overview of microfluidic-based NAT technologies and summarize and discuss the characteristics and development prospects of these techniques.https://www.mdpi.com/2072-666X/13/4/636SARS-CoV-2nucleic acid testingpolymerase chain reactionisothermal amplificationmicrofluidicCRISPR–Cas systems |
| spellingShingle | Weidu Song Taiyi Zhang Huichao Lin Yujing Yang Gaozhen Zhao Xiaowen Huang Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2 Micromachines SARS-CoV-2 nucleic acid testing polymerase chain reaction isothermal amplification microfluidic CRISPR–Cas systems |
| title | Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2 |
| title_full | Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2 |
| title_fullStr | Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2 |
| title_full_unstemmed | Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2 |
| title_short | Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2 |
| title_sort | conventional and microfluidic methods for the detection of nucleic acid of sars cov 2 |
| topic | SARS-CoV-2 nucleic acid testing polymerase chain reaction isothermal amplification microfluidic CRISPR–Cas systems |
| url | https://www.mdpi.com/2072-666X/13/4/636 |
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