Changes in the reproductive function and developmental phenotypes in mice following intramuscular injection of an activin betaA-expressing plasmid
<p>Abstract</p> <p>Background</p> <p>The TGF-beta family protein activin has numerous reported activities with some uncertainty in the reproductive axis and development. The precise roles of activin in in vivo system were investigated using a transient gain of function...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-12-01
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| Series: | Reproductive Biology and Endocrinology |
| Online Access: | http://www.rbej.com/content/6/1/63 |
| _version_ | 1818640465733353472 |
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| author | Mayo Kelly E Cho Chunghee Jung Hoi Park Moon Kim Mi-Nyeu Cho Byung-Nam |
| author_facet | Mayo Kelly E Cho Chunghee Jung Hoi Park Moon Kim Mi-Nyeu Cho Byung-Nam |
| author_sort | Mayo Kelly E |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The TGF-beta family protein activin has numerous reported activities with some uncertainty in the reproductive axis and development. The precise roles of activin in in vivo system were investigated using a transient gain of function model.</p> <p>Methods</p> <p>To this end, an expression plasmid, pCMV-rAct, with the activin betaA cDNA fused to the cytomegalovirus promoter, was introduced into muscle of the female adult mice by direct injection.</p> <p>Results</p> <p>Activin betaA mRNA was detected in the muscle by RT-PCR and subsequent Southern blot analysis. Activin betaA was also detected, and western blot analysis revealed a relatively high level of serum activin with correspondingly increased FSH. In the pCMV-rAct-injected female mice, estrus stage within the estrous cycle was extended. Moreover, increased numbers of corpora lutea and a thickened granulosa cell layer with a small antrum in tertiary follicles within the ovary were observed. When injected female mice were mated with males of proven fertility, a subset of embryos died in utero, and most of those that survived exhibited increased body weight.</p> <p>Conclusion</p> <p>Taken together, our data reveal that activin betaA can directly influence the estrous cycle, an integral part of the reproduction in female mice and activin betaA can also influence the embryo development as an endocrine fashion.</p> |
| first_indexed | 2024-12-16T23:11:42Z |
| format | Article |
| id | doaj.art-08be603739de4acc93b8d3bc02712416 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-16T23:11:42Z |
| publishDate | 2008-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-08be603739de4acc93b8d3bc027124162022-12-21T22:12:24ZengBMCReproductive Biology and Endocrinology1477-78272008-12-01616310.1186/1477-7827-6-63Changes in the reproductive function and developmental phenotypes in mice following intramuscular injection of an activin betaA-expressing plasmidMayo Kelly ECho ChungheeJung HoiPark MoonKim Mi-NyeuCho Byung-Nam<p>Abstract</p> <p>Background</p> <p>The TGF-beta family protein activin has numerous reported activities with some uncertainty in the reproductive axis and development. The precise roles of activin in in vivo system were investigated using a transient gain of function model.</p> <p>Methods</p> <p>To this end, an expression plasmid, pCMV-rAct, with the activin betaA cDNA fused to the cytomegalovirus promoter, was introduced into muscle of the female adult mice by direct injection.</p> <p>Results</p> <p>Activin betaA mRNA was detected in the muscle by RT-PCR and subsequent Southern blot analysis. Activin betaA was also detected, and western blot analysis revealed a relatively high level of serum activin with correspondingly increased FSH. In the pCMV-rAct-injected female mice, estrus stage within the estrous cycle was extended. Moreover, increased numbers of corpora lutea and a thickened granulosa cell layer with a small antrum in tertiary follicles within the ovary were observed. When injected female mice were mated with males of proven fertility, a subset of embryos died in utero, and most of those that survived exhibited increased body weight.</p> <p>Conclusion</p> <p>Taken together, our data reveal that activin betaA can directly influence the estrous cycle, an integral part of the reproduction in female mice and activin betaA can also influence the embryo development as an endocrine fashion.</p>http://www.rbej.com/content/6/1/63 |
| spellingShingle | Mayo Kelly E Cho Chunghee Jung Hoi Park Moon Kim Mi-Nyeu Cho Byung-Nam Changes in the reproductive function and developmental phenotypes in mice following intramuscular injection of an activin betaA-expressing plasmid Reproductive Biology and Endocrinology |
| title | Changes in the reproductive function and developmental phenotypes in mice following intramuscular injection of an activin betaA-expressing plasmid |
| title_full | Changes in the reproductive function and developmental phenotypes in mice following intramuscular injection of an activin betaA-expressing plasmid |
| title_fullStr | Changes in the reproductive function and developmental phenotypes in mice following intramuscular injection of an activin betaA-expressing plasmid |
| title_full_unstemmed | Changes in the reproductive function and developmental phenotypes in mice following intramuscular injection of an activin betaA-expressing plasmid |
| title_short | Changes in the reproductive function and developmental phenotypes in mice following intramuscular injection of an activin betaA-expressing plasmid |
| title_sort | changes in the reproductive function and developmental phenotypes in mice following intramuscular injection of an activin betaa expressing plasmid |
| url | http://www.rbej.com/content/6/1/63 |
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