Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern Thailand
Background and Aim: Mammaliicoccus sciuri, formerly known as Staphylococcus sciuri, is an opportunistic pathogen in the environment, human and animal mucosa, and skin. Although this pathogen is becoming more resistant to drugs and harmful to animals and humans, basic knowledge of this pathogen remai...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-02-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.16/February-2023/7.pdf |
| _version_ | 1797924375254532096 |
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| author | Kanpapat Boonchuay Narin Sontigun Tuempong Wongtawan Punpichaya Fungwithaya |
| author_facet | Kanpapat Boonchuay Narin Sontigun Tuempong Wongtawan Punpichaya Fungwithaya |
| author_sort | Kanpapat Boonchuay |
| collection | DOAJ |
| description | Background and Aim: Mammaliicoccus sciuri, formerly known as Staphylococcus sciuri, is an opportunistic pathogen in the environment, human and animal mucosa, and skin. Although this pathogen is becoming more resistant to drugs and harmful to animals and humans, basic knowledge of this pathogen remains limited. This study aimed to investigate a new multilocus sequencing type (MLST) related to the antibiotic resistance pattern of M. sciuri from animals in southern Thailand.
Materials and Methods: We used 11 methicillin-resistant M. sciuri (MRMS) isolates in this study which were obtained from six horses, four cows, and one chicken of the previous study. Antimicrobial resistance (AMR) was re-evaluated based on the minimum inhibitory concentration using the VITEK® 2 automated system. Three AMR genes were examined, namely mecA, mecC, and blaZ. Staphylococcal chromosomal cassette mec (SCCmec) gene detection was performed through the multiplex polymerase chain reaction (PCR). Internal segments of the seven housekeeping genes, ack, aroE, ftsZ, glpK, gmk, pta1, and tpiA, were used for multilocus sequence typing. The population of resistant bacteria and the types of multidrug-resistant, extensively drug-resistant, and pandemic drug-resistant bacteria were classified through descriptive analysis.
Results: mecA and blaZ genes were detected in all isolates; however, the mecC gene was not observed in any isolate based on the PCR results. All MRMS isolates revealed a non-typable SCCmec. Seven MLSTs (71, 81, 120, 121, 122, 199, and 200) were identified in this study.
Conclusion: The characteristics of MRMS in Southern Thailand were variable, particularly in cattle and horses. The antibiogram and SCCmec types of this pathogen remain concerns with regard to antibiotic-resistant gene transmission among Staphylococcus and Mammaliicoccus species. All MLSTs in Thailand revealed the distribution among clones in Asia, including the virulence of a zoonotic clone in Southern Thailand. |
| first_indexed | 2024-04-10T15:00:30Z |
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| id | doaj.art-08bfd2f565834f5bbc02e85e8e922eb1 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-10T15:00:30Z |
| publishDate | 2023-02-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-08bfd2f565834f5bbc02e85e8e922eb12023-02-15T13:47:25ZengVeterinary WorldVeterinary World0972-89882231-09162023-02-0116229129510.14202/vetworld.2023.291-295Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern ThailandKanpapat Boonchuay0https://orcid.org/0000-0001-5140-429XNarin Sontigun1https://orcid.org/0000-0003-0216-2380Tuempong Wongtawan2https://orcid.org/0000-0001-6948-4144Punpichaya Fungwithaya3https://orcid.org/0000-0003-2412-1061Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand.Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand; Centre of Excellence Research for Melioidosis and Other Microorganisms, Walailak University, Nakhon Si Thammarat 80160, Thailand.Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand; Centre of Excellence Research for Melioidosis and Other Microorganisms, Walailak University, Nakhon Si Thammarat 80160, Thailand.Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand; Centre of Excellence Research for Melioidosis and Other Microorganisms, Walailak University, Nakhon Si Thammarat 80160, Thailand.Background and Aim: Mammaliicoccus sciuri, formerly known as Staphylococcus sciuri, is an opportunistic pathogen in the environment, human and animal mucosa, and skin. Although this pathogen is becoming more resistant to drugs and harmful to animals and humans, basic knowledge of this pathogen remains limited. This study aimed to investigate a new multilocus sequencing type (MLST) related to the antibiotic resistance pattern of M. sciuri from animals in southern Thailand. Materials and Methods: We used 11 methicillin-resistant M. sciuri (MRMS) isolates in this study which were obtained from six horses, four cows, and one chicken of the previous study. Antimicrobial resistance (AMR) was re-evaluated based on the minimum inhibitory concentration using the VITEK® 2 automated system. Three AMR genes were examined, namely mecA, mecC, and blaZ. Staphylococcal chromosomal cassette mec (SCCmec) gene detection was performed through the multiplex polymerase chain reaction (PCR). Internal segments of the seven housekeeping genes, ack, aroE, ftsZ, glpK, gmk, pta1, and tpiA, were used for multilocus sequence typing. The population of resistant bacteria and the types of multidrug-resistant, extensively drug-resistant, and pandemic drug-resistant bacteria were classified through descriptive analysis. Results: mecA and blaZ genes were detected in all isolates; however, the mecC gene was not observed in any isolate based on the PCR results. All MRMS isolates revealed a non-typable SCCmec. Seven MLSTs (71, 81, 120, 121, 122, 199, and 200) were identified in this study. Conclusion: The characteristics of MRMS in Southern Thailand were variable, particularly in cattle and horses. The antibiogram and SCCmec types of this pathogen remain concerns with regard to antibiotic-resistant gene transmission among Staphylococcus and Mammaliicoccus species. All MLSTs in Thailand revealed the distribution among clones in Asia, including the virulence of a zoonotic clone in Southern Thailand.http://www.veterinaryworld.org/Vol.16/February-2023/7.pdfantibiogrammammaliicoccus sciurimethicillin-resistant bacteriamultilocus sequencing typesresistant gene |
| spellingShingle | Kanpapat Boonchuay Narin Sontigun Tuempong Wongtawan Punpichaya Fungwithaya Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern Thailand Veterinary World antibiogram mammaliicoccus sciuri methicillin-resistant bacteria multilocus sequencing types resistant gene |
| title | Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern Thailand |
| title_full | Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern Thailand |
| title_fullStr | Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern Thailand |
| title_full_unstemmed | Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern Thailand |
| title_short | Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern Thailand |
| title_sort | association of multilocus sequencing types and antimicrobial resistance profiles of methicillin resistant mammaliicoccus sciuri in animals in southern thailand |
| topic | antibiogram mammaliicoccus sciuri methicillin-resistant bacteria multilocus sequencing types resistant gene |
| url | http://www.veterinaryworld.org/Vol.16/February-2023/7.pdf |
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