The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition
Kinesins that influence the dynamics of microtubule growth and shrinkage require the ability to distinguish between the microtubule end and the microtubule lattice. The microtubule depolymerizing kinesin MCAK has been shown to specifically recognize the microtubule end. This ability is key to the ac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2016-01-01
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| Series: | Open Biology |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160223 |
| _version_ | 1818884701182492672 |
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| author | Jennifer T. Patel Hannah R. Belsham Alexandra J. Rathbone Bill Wickstead Christopher Gell Claire T. Friel |
| author_facet | Jennifer T. Patel Hannah R. Belsham Alexandra J. Rathbone Bill Wickstead Christopher Gell Claire T. Friel |
| author_sort | Jennifer T. Patel |
| collection | DOAJ |
| description | Kinesins that influence the dynamics of microtubule growth and shrinkage require the ability to distinguish between the microtubule end and the microtubule lattice. The microtubule depolymerizing kinesin MCAK has been shown to specifically recognize the microtubule end. This ability is key to the action of MCAK in regulating microtubule dynamics. We show that the α4-helix of the motor domain is crucial to microtubule end recognition. Mutation of the residues K524, E525 and R528, which are located in the C-terminal half of the α4-helix, specifically disrupts the ability of MCAK to recognize the microtubule end. Mutation of these residues, which are conserved in the kinesin-13 family and discriminate members of this family from translocating kinesins, impairs the ability of MCAK to discriminate between the microtubule lattice and the microtubule end. |
| first_indexed | 2024-12-19T15:53:44Z |
| format | Article |
| id | doaj.art-08c0f014e14f4239ae8fc59ff8100878 |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-12-19T15:53:44Z |
| publishDate | 2016-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-08c0f014e14f4239ae8fc59ff81008782022-12-21T20:15:09ZengThe Royal SocietyOpen Biology2046-24412016-01-0161010.1098/rsob.160223160223The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognitionJennifer T. PatelHannah R. BelshamAlexandra J. RathboneBill WicksteadChristopher GellClaire T. FrielKinesins that influence the dynamics of microtubule growth and shrinkage require the ability to distinguish between the microtubule end and the microtubule lattice. The microtubule depolymerizing kinesin MCAK has been shown to specifically recognize the microtubule end. This ability is key to the action of MCAK in regulating microtubule dynamics. We show that the α4-helix of the motor domain is crucial to microtubule end recognition. Mutation of the residues K524, E525 and R528, which are located in the C-terminal half of the α4-helix, specifically disrupts the ability of MCAK to recognize the microtubule end. Mutation of these residues, which are conserved in the kinesin-13 family and discriminate members of this family from translocating kinesins, impairs the ability of MCAK to discriminate between the microtubule lattice and the microtubule end.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160223mcakkinesin-13microtubuledepolymerizationatp turnovermicrotubule end recognition |
| spellingShingle | Jennifer T. Patel Hannah R. Belsham Alexandra J. Rathbone Bill Wickstead Christopher Gell Claire T. Friel The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition Open Biology mcak kinesin-13 microtubule depolymerization atp turnover microtubule end recognition |
| title | The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition |
| title_full | The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition |
| title_fullStr | The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition |
| title_full_unstemmed | The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition |
| title_short | The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition |
| title_sort | family specific α4 helix of the kinesin 13 mcak is critical to microtubule end recognition |
| topic | mcak kinesin-13 microtubule depolymerization atp turnover microtubule end recognition |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160223 |
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