A Novel c.100C > G Mutation in the Gene and Its Relation With the Reproductive Traits of Awassi Ewes
Reproductive traits are affected by many factors, including ovarian function, hormones, and genetics. Genetic polymorphisms of candidate genes are associated with reproductive traits. Several candidate genes are associated with economic traits, including the follistatin ( FST ) gene. Thus, this stud...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-05-01
|
| Series: | Bioinformatics and Biology Insights |
| Online Access: | https://doi.org/10.1177/11779322231170988 |
| _version_ | 1827955570773065728 |
|---|---|
| author | Tahreer M Al-Thuwaini Wefak J Albazi Mohammed Baqur S Al-Shuhaib Layth H Merzah Rihab G Mohammed Fadhil A Rhadi Ali B Abd Al-Hadi Ahmed H Alkhammas |
| author_facet | Tahreer M Al-Thuwaini Wefak J Albazi Mohammed Baqur S Al-Shuhaib Layth H Merzah Rihab G Mohammed Fadhil A Rhadi Ali B Abd Al-Hadi Ahmed H Alkhammas |
| author_sort | Tahreer M Al-Thuwaini |
| collection | DOAJ |
| description | Reproductive traits are affected by many factors, including ovarian function, hormones, and genetics. Genetic polymorphisms of candidate genes are associated with reproductive traits. Several candidate genes are associated with economic traits, including the follistatin ( FST ) gene. Thus, this study aimed to evaluate whether the genetic variations in the FST gene are associated with the reproductive traits in Awassi ewes. The genomic DNA was extracted from 109 twin ewes and 123 single-progeny ewes. Therefore, 4 sequence fragments from the FST gene were amplified using polymerase chain reaction (PCR) (exon 2/240, exon 3/268, exon 4/254, and exon 5/266 bp, respectively). For a 254 bp amplicon, 3 genotypes were identified: CC, CG, and GG. Sequencing revealed a novel mutation in CG genotypes c.100C > G. The statistical analysis of c.100C > G showed an association with reproductive characteristics. Ewes carrying the c.100C > G had significantly ( P ⩽ .01) lower litter sizes, twinning rates, lambing rates, and more days to lambing compared with CG and CC genotypes. Logistic regression analysis confirmed that the c.100C > G single-nucleotide polymorphism (SNP) is responsible for decreasing litter size. According to these results, the variant c.100C > G negatively affects the traits of interest and is associated with lower reproductive traits in Awassi sheep. As a result of this study, ewes carrying the c.100C > G SNP have lower litter size and are less prolific. |
| first_indexed | 2024-04-09T14:49:31Z |
| format | Article |
| id | doaj.art-08c12629b5884bd9a70a561048ef80a3 |
| institution | Directory Open Access Journal |
| issn | 1177-9322 |
| language | English |
| last_indexed | 2024-04-09T14:49:31Z |
| publishDate | 2023-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Bioinformatics and Biology Insights |
| spelling | doaj.art-08c12629b5884bd9a70a561048ef80a32023-05-02T09:46:57ZengSAGE PublishingBioinformatics and Biology Insights1177-93222023-05-011710.1177/11779322231170988A Novel c.100C > G Mutation in the Gene and Its Relation With the Reproductive Traits of Awassi EwesTahreer M Al-Thuwaini0Wefak J Albazi1Mohammed Baqur S Al-Shuhaib2Layth H Merzah3Rihab G Mohammed4Fadhil A Rhadi5Ali B Abd Al-Hadi6Ahmed H Alkhammas7Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, IraqDepartment of Physiology, College of Veterinary Medicine, University of Kerbala, Kerbala, IraqDepartment of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, IraqDepartment of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, IraqDepartment of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, IraqDepartment of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, IraqDepartment of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, IraqDepartment of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, IraqReproductive traits are affected by many factors, including ovarian function, hormones, and genetics. Genetic polymorphisms of candidate genes are associated with reproductive traits. Several candidate genes are associated with economic traits, including the follistatin ( FST ) gene. Thus, this study aimed to evaluate whether the genetic variations in the FST gene are associated with the reproductive traits in Awassi ewes. The genomic DNA was extracted from 109 twin ewes and 123 single-progeny ewes. Therefore, 4 sequence fragments from the FST gene were amplified using polymerase chain reaction (PCR) (exon 2/240, exon 3/268, exon 4/254, and exon 5/266 bp, respectively). For a 254 bp amplicon, 3 genotypes were identified: CC, CG, and GG. Sequencing revealed a novel mutation in CG genotypes c.100C > G. The statistical analysis of c.100C > G showed an association with reproductive characteristics. Ewes carrying the c.100C > G had significantly ( P ⩽ .01) lower litter sizes, twinning rates, lambing rates, and more days to lambing compared with CG and CC genotypes. Logistic regression analysis confirmed that the c.100C > G single-nucleotide polymorphism (SNP) is responsible for decreasing litter size. According to these results, the variant c.100C > G negatively affects the traits of interest and is associated with lower reproductive traits in Awassi sheep. As a result of this study, ewes carrying the c.100C > G SNP have lower litter size and are less prolific.https://doi.org/10.1177/11779322231170988 |
| spellingShingle | Tahreer M Al-Thuwaini Wefak J Albazi Mohammed Baqur S Al-Shuhaib Layth H Merzah Rihab G Mohammed Fadhil A Rhadi Ali B Abd Al-Hadi Ahmed H Alkhammas A Novel c.100C > G Mutation in the Gene and Its Relation With the Reproductive Traits of Awassi Ewes Bioinformatics and Biology Insights |
| title | A Novel c.100C > G Mutation in the Gene and Its Relation With the Reproductive Traits of Awassi Ewes |
| title_full | A Novel c.100C > G Mutation in the Gene and Its Relation With the Reproductive Traits of Awassi Ewes |
| title_fullStr | A Novel c.100C > G Mutation in the Gene and Its Relation With the Reproductive Traits of Awassi Ewes |
| title_full_unstemmed | A Novel c.100C > G Mutation in the Gene and Its Relation With the Reproductive Traits of Awassi Ewes |
| title_short | A Novel c.100C > G Mutation in the Gene and Its Relation With the Reproductive Traits of Awassi Ewes |
| title_sort | novel c 100c g mutation in the gene and its relation with the reproductive traits of awassi ewes |
| url | https://doi.org/10.1177/11779322231170988 |
| work_keys_str_mv | AT tahreermalthuwaini anovelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT wefakjalbazi anovelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT mohammedbaqursalshuhaib anovelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT laythhmerzah anovelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT rihabgmohammed anovelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT fadhilarhadi anovelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT alibabdalhadi anovelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT ahmedhalkhammas anovelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT tahreermalthuwaini novelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT wefakjalbazi novelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT mohammedbaqursalshuhaib novelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT laythhmerzah novelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT rihabgmohammed novelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT fadhilarhadi novelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT alibabdalhadi novelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes AT ahmedhalkhammas novelc100cgmutationinthegeneanditsrelationwiththereproductivetraitsofawassiewes |