The heavy metal-associated isoprenylated plant protein (HIPP) gene family plays a crucial role in cadmium resistance and accumulation in the tea plant (Camellia sinensis L.)
Heavy metal-associated isoprenylated plant proteins (HIPPs) are only distributed in vascular plants, and are essential for the detoxification and vascular transport of heavy metals in plants. However, the HIPP gene family has not been thoroughly explored in the tea plant (Camellia sinensis). In this...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S014765132300581X |
| _version_ | 1827928312321671168 |
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| author | Yunfeng Wei Xuqian Peng Xiaojing Wang Cheng Wang |
| author_facet | Yunfeng Wei Xuqian Peng Xiaojing Wang Cheng Wang |
| author_sort | Yunfeng Wei |
| collection | DOAJ |
| description | Heavy metal-associated isoprenylated plant proteins (HIPPs) are only distributed in vascular plants, and are essential for the detoxification and vascular transport of heavy metals in plants. However, the HIPP gene family has not been thoroughly explored in the tea plant (Camellia sinensis). In this study, we systematically identified 56C. sinensis CsHIPP genes from five groups and characterized their phylogeny, structures, and the features of the encoded proteins. The expression patterns of CsHIPP genes in various tissues of C. sinensis were investigated based on a previous RNA-seq data analysis. The expression patterns of CsHIPP genes were explored in cadmium (Cd)-treated C. sinensis roots using our RNA-seq data. Three CsHIPP genes (CsHIPP22, CsHIPP24, and CsHIPP36) with high expression levels in Cd-treated C. sinensis roots were selected as candidate genes associated with Cd tolerance. Overexpression of CsHIPP22, CsHIPP24, and CsHIPP36 in a yeast mutant (ycf1) rescued Cd-sensitive ycf1 yeast and increased the yeast resistance to Cd stress, implying that these three CsHIPPs might be involved in Cd tolerance. These findings will enable the roles of HIPPs in Cd absorption and detoxification to be better understood as well as improving our understanding of the Cd-resistance and Cd-accumulation mechanisms in tea plant. |
| first_indexed | 2024-03-13T06:05:55Z |
| format | Article |
| id | doaj.art-08c2105b86be40b9a9ede85342d14504 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-13T06:05:55Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-08c2105b86be40b9a9ede85342d145042023-06-12T04:08:58ZengElsevierEcotoxicology and Environmental Safety0147-65132023-07-01260115077The heavy metal-associated isoprenylated plant protein (HIPP) gene family plays a crucial role in cadmium resistance and accumulation in the tea plant (Camellia sinensis L.)Yunfeng Wei0Xuqian Peng1Xiaojing Wang2Cheng Wang3College of Tea Science, Guizhou University, Guiyang 550025, ChinaCollege of Tea Science, Guizhou University, Guiyang 550025, ChinaCollege of Tea Science, Guizhou University, Guiyang 550025, China; Corresponding authors.Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, College of Life Scienceand Technology, Hubei EngineeringUniversity, Xiaogan 432000, China; Corresponding authors.Heavy metal-associated isoprenylated plant proteins (HIPPs) are only distributed in vascular plants, and are essential for the detoxification and vascular transport of heavy metals in plants. However, the HIPP gene family has not been thoroughly explored in the tea plant (Camellia sinensis). In this study, we systematically identified 56C. sinensis CsHIPP genes from five groups and characterized their phylogeny, structures, and the features of the encoded proteins. The expression patterns of CsHIPP genes in various tissues of C. sinensis were investigated based on a previous RNA-seq data analysis. The expression patterns of CsHIPP genes were explored in cadmium (Cd)-treated C. sinensis roots using our RNA-seq data. Three CsHIPP genes (CsHIPP22, CsHIPP24, and CsHIPP36) with high expression levels in Cd-treated C. sinensis roots were selected as candidate genes associated with Cd tolerance. Overexpression of CsHIPP22, CsHIPP24, and CsHIPP36 in a yeast mutant (ycf1) rescued Cd-sensitive ycf1 yeast and increased the yeast resistance to Cd stress, implying that these three CsHIPPs might be involved in Cd tolerance. These findings will enable the roles of HIPPs in Cd absorption and detoxification to be better understood as well as improving our understanding of the Cd-resistance and Cd-accumulation mechanisms in tea plant.http://www.sciencedirect.com/science/article/pii/S014765132300581XCsHIPP gene familyExpression patternCd stressOverexpressionCd-sensitive ycf1 yeast |
| spellingShingle | Yunfeng Wei Xuqian Peng Xiaojing Wang Cheng Wang The heavy metal-associated isoprenylated plant protein (HIPP) gene family plays a crucial role in cadmium resistance and accumulation in the tea plant (Camellia sinensis L.) Ecotoxicology and Environmental Safety CsHIPP gene family Expression pattern Cd stress Overexpression Cd-sensitive ycf1 yeast |
| title | The heavy metal-associated isoprenylated plant protein (HIPP) gene family plays a crucial role in cadmium resistance and accumulation in the tea plant (Camellia sinensis L.) |
| title_full | The heavy metal-associated isoprenylated plant protein (HIPP) gene family plays a crucial role in cadmium resistance and accumulation in the tea plant (Camellia sinensis L.) |
| title_fullStr | The heavy metal-associated isoprenylated plant protein (HIPP) gene family plays a crucial role in cadmium resistance and accumulation in the tea plant (Camellia sinensis L.) |
| title_full_unstemmed | The heavy metal-associated isoprenylated plant protein (HIPP) gene family plays a crucial role in cadmium resistance and accumulation in the tea plant (Camellia sinensis L.) |
| title_short | The heavy metal-associated isoprenylated plant protein (HIPP) gene family plays a crucial role in cadmium resistance and accumulation in the tea plant (Camellia sinensis L.) |
| title_sort | heavy metal associated isoprenylated plant protein hipp gene family plays a crucial role in cadmium resistance and accumulation in the tea plant camellia sinensis l |
| topic | CsHIPP gene family Expression pattern Cd stress Overexpression Cd-sensitive ycf1 yeast |
| url | http://www.sciencedirect.com/science/article/pii/S014765132300581X |
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