In Vivo Motility Patterns Displayed by Immune Cells Under Inflammatory Conditions
The migration of immune cells plays a key role in inflammation. This is evident in the fact that inflammatory stimuli elicit a broad range of migration patterns in immune cells. Since these patterns are pivotal for initiating the immune response, their dysregulation is associated with life-threateni...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.804159/full |
| _version_ | 1818285409763852288 |
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| author | Diego Ulisse Pizzagalli Diego Ulisse Pizzagalli Alain Pulfer Alain Pulfer Marcus Thelen Rolf Krause Santiago F. Gonzalez |
| author_facet | Diego Ulisse Pizzagalli Diego Ulisse Pizzagalli Alain Pulfer Alain Pulfer Marcus Thelen Rolf Krause Santiago F. Gonzalez |
| author_sort | Diego Ulisse Pizzagalli |
| collection | DOAJ |
| description | The migration of immune cells plays a key role in inflammation. This is evident in the fact that inflammatory stimuli elicit a broad range of migration patterns in immune cells. Since these patterns are pivotal for initiating the immune response, their dysregulation is associated with life-threatening conditions including organ failure, chronic inflammation, autoimmunity, and cancer, amongst others. Over the last two decades, thanks to advancements in the intravital microscopy technology, it has become possible to visualize cell migration in living organisms with unprecedented resolution, helping to deconstruct hitherto unexplored aspects of the immune response associated with the dynamism of cells. However, a comprehensive classification of the main motility patterns of immune cells observed in vivo, along with their relevance to the inflammatory process, is still lacking. In this review we defined cell actions as motility patterns displayed by immune cells, which are associated with a specific role during the immune response. In this regard, we summarize the main actions performed by immune cells during intravital microscopy studies. For each of these actions, we provide a consensus name, a definition based on morphodynamic properties, and the biological contexts in which it was reported. Moreover, we provide an overview of the computational methods that were employed for the quantification, fostering an interdisciplinary approach to study the immune system from imaging data. |
| first_indexed | 2024-12-13T01:08:15Z |
| format | Article |
| id | doaj.art-08c33b7efecd4172ac5f7ee0865370f4 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-13T01:08:15Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-08c33b7efecd4172ac5f7ee0865370f42022-12-22T00:04:31ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-01-011210.3389/fimmu.2021.804159804159In Vivo Motility Patterns Displayed by Immune Cells Under Inflammatory ConditionsDiego Ulisse Pizzagalli0Diego Ulisse Pizzagalli1Alain Pulfer2Alain Pulfer3Marcus Thelen4Rolf Krause5Santiago F. Gonzalez6Istituto di Ricerca in Biomedicina (IRB), Università della Svizzera italiana, Bellinzona, SwitzerlandEuler institute, Università della Svizzera italiana, Lugano-Viganello, SwitzerlandIstituto di Ricerca in Biomedicina (IRB), Università della Svizzera italiana, Bellinzona, SwitzerlandDepartment of Information Technology and Electrical Engineering, Swiss Federal Institute of Technology Zurich (ETHZ) Zürich, Zürich, SwitzerlandIstituto di Ricerca in Biomedicina (IRB), Università della Svizzera italiana, Bellinzona, SwitzerlandEuler institute, Università della Svizzera italiana, Lugano-Viganello, SwitzerlandIstituto di Ricerca in Biomedicina (IRB), Università della Svizzera italiana, Bellinzona, SwitzerlandThe migration of immune cells plays a key role in inflammation. This is evident in the fact that inflammatory stimuli elicit a broad range of migration patterns in immune cells. Since these patterns are pivotal for initiating the immune response, their dysregulation is associated with life-threatening conditions including organ failure, chronic inflammation, autoimmunity, and cancer, amongst others. Over the last two decades, thanks to advancements in the intravital microscopy technology, it has become possible to visualize cell migration in living organisms with unprecedented resolution, helping to deconstruct hitherto unexplored aspects of the immune response associated with the dynamism of cells. However, a comprehensive classification of the main motility patterns of immune cells observed in vivo, along with their relevance to the inflammatory process, is still lacking. In this review we defined cell actions as motility patterns displayed by immune cells, which are associated with a specific role during the immune response. In this regard, we summarize the main actions performed by immune cells during intravital microscopy studies. For each of these actions, we provide a consensus name, a definition based on morphodynamic properties, and the biological contexts in which it was reported. Moreover, we provide an overview of the computational methods that were employed for the quantification, fostering an interdisciplinary approach to study the immune system from imaging data.https://www.frontiersin.org/articles/10.3389/fimmu.2021.804159/fullcell actionscomputer visioninflammationintravital imagingleukocytesmotility patterns |
| spellingShingle | Diego Ulisse Pizzagalli Diego Ulisse Pizzagalli Alain Pulfer Alain Pulfer Marcus Thelen Rolf Krause Santiago F. Gonzalez In Vivo Motility Patterns Displayed by Immune Cells Under Inflammatory Conditions Frontiers in Immunology cell actions computer vision inflammation intravital imaging leukocytes motility patterns |
| title | In Vivo Motility Patterns Displayed by Immune Cells Under Inflammatory Conditions |
| title_full | In Vivo Motility Patterns Displayed by Immune Cells Under Inflammatory Conditions |
| title_fullStr | In Vivo Motility Patterns Displayed by Immune Cells Under Inflammatory Conditions |
| title_full_unstemmed | In Vivo Motility Patterns Displayed by Immune Cells Under Inflammatory Conditions |
| title_short | In Vivo Motility Patterns Displayed by Immune Cells Under Inflammatory Conditions |
| title_sort | in vivo motility patterns displayed by immune cells under inflammatory conditions |
| topic | cell actions computer vision inflammation intravital imaging leukocytes motility patterns |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.804159/full |
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