Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase
The electrochemical detection of interactions between aptamers and low-molecular-weight targets often lacks sensitivity. Signal amplification improves the detection of the aptamer-analyte complex; Bsm DNA polymerase was used to amplify the signal from the interaction of vanillin and its aptamer name...
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| Format: | Article |
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MDPI AG
2017-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/18/1/49 |
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| author | Mariia Andrianova Natalia Komarova Vitaliy Grudtsov Evgeniy Kuznetsov Alexander Kuznetsov |
| author_facet | Mariia Andrianova Natalia Komarova Vitaliy Grudtsov Evgeniy Kuznetsov Alexander Kuznetsov |
| author_sort | Mariia Andrianova |
| collection | DOAJ |
| description | The electrochemical detection of interactions between aptamers and low-molecular-weight targets often lacks sensitivity. Signal amplification improves the detection of the aptamer-analyte complex; Bsm DNA polymerase was used to amplify the signal from the interaction of vanillin and its aptamer named Van_74 on an ion-sensitive field-effect transistor (ISFET)-based biosensor. The aptamer was immobilized on the ISFET sensitive surface. A short DNA probe was hybridized with the aptamer and dissociated from it upon vanillin addition. A free probe interacted with a special DNA molecular beacon initiated the Bsm DNA polymerase reaction that was detected by ISFET. A buffer solution suitable for both aptamer action and Bsm DNA polymerase activity was determined. The ISFET was shown to detect the Bsm DNA polymerase reaction under the selected conditions. Vanillin at different concentrations (1 × 10−6–1 × 10−8 M) was detected using the biosensor with signal amplification. The developed detection system allowed for the determination of vanillin, starting at a 10−8 M concentration. Application of the Bsm DNA polymerase resulted in a 15.5 times lower LoD when compared to the biosensor without signal amplification (10.1007/s00604-017-2586-4). |
| first_indexed | 2024-04-12T05:40:34Z |
| format | Article |
| id | doaj.art-08caaeb216304d2dae5de825843dbf73 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-12T05:40:34Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-08caaeb216304d2dae5de825843dbf732022-12-22T03:45:40ZengMDPI AGSensors1424-82202017-12-011814910.3390/s18010049s18010049Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA PolymeraseMariia Andrianova0Natalia Komarova1Vitaliy Grudtsov2Evgeniy Kuznetsov3Alexander Kuznetsov4Scientific-Manufacturing Complex Technological Centre, 1–7 Shokin Square, Zelenograd, 124498 Moscow, RussiaScientific-Manufacturing Complex Technological Centre, 1–7 Shokin Square, Zelenograd, 124498 Moscow, RussiaScientific-Manufacturing Complex Technological Centre, 1–7 Shokin Square, Zelenograd, 124498 Moscow, RussiaScientific-Manufacturing Complex Technological Centre, 1–7 Shokin Square, Zelenograd, 124498 Moscow, RussiaScientific-Manufacturing Complex Technological Centre, 1–7 Shokin Square, Zelenograd, 124498 Moscow, RussiaThe electrochemical detection of interactions between aptamers and low-molecular-weight targets often lacks sensitivity. Signal amplification improves the detection of the aptamer-analyte complex; Bsm DNA polymerase was used to amplify the signal from the interaction of vanillin and its aptamer named Van_74 on an ion-sensitive field-effect transistor (ISFET)-based biosensor. The aptamer was immobilized on the ISFET sensitive surface. A short DNA probe was hybridized with the aptamer and dissociated from it upon vanillin addition. A free probe interacted with a special DNA molecular beacon initiated the Bsm DNA polymerase reaction that was detected by ISFET. A buffer solution suitable for both aptamer action and Bsm DNA polymerase activity was determined. The ISFET was shown to detect the Bsm DNA polymerase reaction under the selected conditions. Vanillin at different concentrations (1 × 10−6–1 × 10−8 M) was detected using the biosensor with signal amplification. The developed detection system allowed for the determination of vanillin, starting at a 10−8 M concentration. Application of the Bsm DNA polymerase resulted in a 15.5 times lower LoD when compared to the biosensor without signal amplification (10.1007/s00604-017-2586-4).https://www.mdpi.com/1424-8220/18/1/49aptasensoraptamersignal amplificationBsm DNA polymeraseISFETvanillin |
| spellingShingle | Mariia Andrianova Natalia Komarova Vitaliy Grudtsov Evgeniy Kuznetsov Alexander Kuznetsov Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase Sensors aptasensor aptamer signal amplification Bsm DNA polymerase ISFET vanillin |
| title | Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase |
| title_full | Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase |
| title_fullStr | Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase |
| title_full_unstemmed | Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase |
| title_short | Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase |
| title_sort | amplified detection of the aptamer vanillin complex with the use of bsm dna polymerase |
| topic | aptasensor aptamer signal amplification Bsm DNA polymerase ISFET vanillin |
| url | https://www.mdpi.com/1424-8220/18/1/49 |
| work_keys_str_mv | AT mariiaandrianova amplifieddetectionoftheaptamervanillincomplexwiththeuseofbsmdnapolymerase AT nataliakomarova amplifieddetectionoftheaptamervanillincomplexwiththeuseofbsmdnapolymerase AT vitaliygrudtsov amplifieddetectionoftheaptamervanillincomplexwiththeuseofbsmdnapolymerase AT evgeniykuznetsov amplifieddetectionoftheaptamervanillincomplexwiththeuseofbsmdnapolymerase AT alexanderkuznetsov amplifieddetectionoftheaptamervanillincomplexwiththeuseofbsmdnapolymerase |