Molecular characterization of heat tolerance in eggplant (Solanum melongena) lines using SSR markers
High temperature is one of the most important environmental stresses causing heavy reduction in yield of eggplant (Solanum melongena L.) in India. Therefore, development of heat tolerant eggplant variety is an important breeding objective. The present investigation was conducted to assess genetic va...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2020-12-01
|
| Series: | The Indian Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/107908 |
| _version_ | 1811164756738834432 |
|---|---|
| author | SANTHIYA S SAHA P TOMAR B S SAHA N D CHANDRIKA GHOSHAL SUMAN LATA |
| author_facet | SANTHIYA S SAHA P TOMAR B S SAHA N D CHANDRIKA GHOSHAL SUMAN LATA |
| author_sort | SANTHIYA S |
| collection | DOAJ |
| description | High temperature is one of the most important environmental stresses causing heavy reduction in yield of eggplant (Solanum melongena L.) in India. Therefore, development of heat tolerant eggplant variety is an important breeding objective. The present investigation was conducted to assess genetic variation among 62 eggplant lines including heat tolerant and susceptible lines in order to identify novel heat tolerant lines in changing climate in India. We employed 15 simple sequence repeat (SSR) markers to study polymorphisms among eggplant lines obtained from eggplant breeding program. The observations were analyzed using Power Marker version 3.5. The range of amplified number of bands was from 2 to 3 in each of 15 markers. The allele frequency was ranged from 0.5 to 0.7931 with an average of 2.067 alleles per locus. Among the 15 polymorphic SSR markers, 67% markers had PIC value higher than 0.3 with heterozygosity of individual loci was ranged from 0.00 to 0.1667. The marker emf01C03 had higher number of allele (3) and the marker emd03C01 had maximum PIC value and gene diversity. The NJ dendrogram obtained by 15 SSR markers divides 62 lines into 7 clusters where the cluster VII comprised of most heat tolerant lines (DBL-21, Guhala Chatua Local). The amplification pattern of heat tolerant lines also differ which implies different genetic architecture of heat tolerant lines. The research work will be very useful to combine these diverse sources of genetic variability to develop heat tolerant variety (s) in eggplant. |
| first_indexed | 2024-04-10T15:26:31Z |
| format | Article |
| id | doaj.art-08cdf942d1f047e4903acb14f7987a4d |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T15:26:31Z |
| publishDate | 2020-12-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-08cdf942d1f047e4903acb14f7987a4d2023-02-14T08:26:45ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192020-12-01901010.56093/ijas.v90i10.107908Molecular characterization of heat tolerance in eggplant (Solanum melongena) lines using SSR markersSANTHIYA S0SAHA P1TOMAR B S2SAHA N D3CHANDRIKA GHOSHAL4SUMAN LATA5ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaPrincipal Scientist and Head, Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaScientist, CESCRAICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaScientist, Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaHigh temperature is one of the most important environmental stresses causing heavy reduction in yield of eggplant (Solanum melongena L.) in India. Therefore, development of heat tolerant eggplant variety is an important breeding objective. The present investigation was conducted to assess genetic variation among 62 eggplant lines including heat tolerant and susceptible lines in order to identify novel heat tolerant lines in changing climate in India. We employed 15 simple sequence repeat (SSR) markers to study polymorphisms among eggplant lines obtained from eggplant breeding program. The observations were analyzed using Power Marker version 3.5. The range of amplified number of bands was from 2 to 3 in each of 15 markers. The allele frequency was ranged from 0.5 to 0.7931 with an average of 2.067 alleles per locus. Among the 15 polymorphic SSR markers, 67% markers had PIC value higher than 0.3 with heterozygosity of individual loci was ranged from 0.00 to 0.1667. The marker emf01C03 had higher number of allele (3) and the marker emd03C01 had maximum PIC value and gene diversity. The NJ dendrogram obtained by 15 SSR markers divides 62 lines into 7 clusters where the cluster VII comprised of most heat tolerant lines (DBL-21, Guhala Chatua Local). The amplification pattern of heat tolerant lines also differ which implies different genetic architecture of heat tolerant lines. The research work will be very useful to combine these diverse sources of genetic variability to develop heat tolerant variety (s) in eggplant.https://epubs.icar.org.in/index.php/IJAgS/article/view/107908EggplantHeat toleranceSSRVariability |
| spellingShingle | SANTHIYA S SAHA P TOMAR B S SAHA N D CHANDRIKA GHOSHAL SUMAN LATA Molecular characterization of heat tolerance in eggplant (Solanum melongena) lines using SSR markers The Indian Journal of Agricultural Sciences Eggplant Heat tolerance SSR Variability |
| title | Molecular characterization of heat tolerance in eggplant (Solanum melongena) lines using SSR markers |
| title_full | Molecular characterization of heat tolerance in eggplant (Solanum melongena) lines using SSR markers |
| title_fullStr | Molecular characterization of heat tolerance in eggplant (Solanum melongena) lines using SSR markers |
| title_full_unstemmed | Molecular characterization of heat tolerance in eggplant (Solanum melongena) lines using SSR markers |
| title_short | Molecular characterization of heat tolerance in eggplant (Solanum melongena) lines using SSR markers |
| title_sort | molecular characterization of heat tolerance in eggplant solanum melongena lines using ssr markers |
| topic | Eggplant Heat tolerance SSR Variability |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/107908 |
| work_keys_str_mv | AT santhiyas molecularcharacterizationofheattoleranceineggplantsolanummelongenalinesusingssrmarkers AT sahap molecularcharacterizationofheattoleranceineggplantsolanummelongenalinesusingssrmarkers AT tomarbs molecularcharacterizationofheattoleranceineggplantsolanummelongenalinesusingssrmarkers AT sahand molecularcharacterizationofheattoleranceineggplantsolanummelongenalinesusingssrmarkers AT chandrikaghoshal molecularcharacterizationofheattoleranceineggplantsolanummelongenalinesusingssrmarkers AT sumanlata molecularcharacterizationofheattoleranceineggplantsolanummelongenalinesusingssrmarkers |