Culicoides midges (Diptera: Ceratopogonidae) as vectors of orbiviruses in Slovakia
In recent years, rapid spread of Culicoides-borne pathogens such as bluetongue (BT) and Schmallenberg viruses have been reported in Europe. In this study we examined the Culicoides populations in farms with wild and domestic ruminants in Eastern Slovakia with the aim to confirm the presence of bitin...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2014-09-01
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| Series: | Veterinaria Italiana |
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| Online Access: | http://www.izs.it/vet_italiana/2014/50_3/VetIt_2405_11.pdf |
| _version_ | 1819210915707355136 |
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| author | Adela Sarvašová Maria Goffredo Igor Sopoliga Giovanni Savini Alica Kočišová |
| author_facet | Adela Sarvašová Maria Goffredo Igor Sopoliga Giovanni Savini Alica Kočišová |
| author_sort | Adela Sarvašová |
| collection | DOAJ |
| description | In recent years, rapid spread of Culicoides-borne pathogens such as bluetongue (BT) and Schmallenberg viruses have been reported in Europe. In this study we examined the Culicoides populations in farms with wild and domestic ruminants in Eastern Slovakia with the aim to confirm the presence of biting midges serving as potential vectors of important pathogens. The main vector complexes were the Obsoletus complex (54%; n=4,209) and the Pulicaris complex (23%; n=1,796). To estimate the relative abundance of the cryptic species of the Obsoletus complex (Culicoides obsoletus, Culicoides scoticus and Culicoides montanus), we performed the multiplex polymerase chain reaction (PCR) based on ITS-2 and ITS-1 segments, on 125 midges randomly sampled. The relative abundance of C. obsoletus ranged from 5.26% in the farm with wild ruminants to 85.71% in another farm with cattle and sheep. A total of 112 pools of parous and gravid females belonging to the Obsoletus and Pulicaris complexes were tested for virus detection by the real-time reverse transcription polymerase chain reaction (RT-PCR) for BT virus, as well as for the Epizootic Hemorrhagic Disease Virus (EHDV), with negative results. |
| first_indexed | 2024-12-23T06:18:46Z |
| format | Article |
| id | doaj.art-08d04f0d97b640c195c7271389239108 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-12-23T06:18:46Z |
| publishDate | 2014-09-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-08d04f0d97b640c195c72713892391082022-12-21T17:57:14ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272014-09-0150320321210.12834/VetIt.2405.11 Culicoides midges (Diptera: Ceratopogonidae) as vectors of orbiviruses in SlovakiaAdela Sarvašová0Maria Goffredo1Igor Sopoliga2Giovanni Savini3Alica Kočišová4University of Veterinary Medicine and Pharmacy in Košice, Department of Parasitology, Komenského 73, 041 81 Košice, Slovak RepublicIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', 64100 Teramo, ItalyUniversity of Veterinary Medicine and Pharmacy in Košice, Special Centre for study of Breeding and Diseases of Wild Animals, Fish and Bees, Rozhanovce, Slovak RepublicIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', 64100 Teramo, ItalyDepartment of Parasitology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovak Republic. Tel.: +421915984641, e-mail: alica.kocisova@uvlf.skIn recent years, rapid spread of Culicoides-borne pathogens such as bluetongue (BT) and Schmallenberg viruses have been reported in Europe. In this study we examined the Culicoides populations in farms with wild and domestic ruminants in Eastern Slovakia with the aim to confirm the presence of biting midges serving as potential vectors of important pathogens. The main vector complexes were the Obsoletus complex (54%; n=4,209) and the Pulicaris complex (23%; n=1,796). To estimate the relative abundance of the cryptic species of the Obsoletus complex (Culicoides obsoletus, Culicoides scoticus and Culicoides montanus), we performed the multiplex polymerase chain reaction (PCR) based on ITS-2 and ITS-1 segments, on 125 midges randomly sampled. The relative abundance of C. obsoletus ranged from 5.26% in the farm with wild ruminants to 85.71% in another farm with cattle and sheep. A total of 112 pools of parous and gravid females belonging to the Obsoletus and Pulicaris complexes were tested for virus detection by the real-time reverse transcription polymerase chain reaction (RT-PCR) for BT virus, as well as for the Epizootic Hemorrhagic Disease Virus (EHDV), with negative results.http://www.izs.it/vet_italiana/2014/50_3/VetIt_2405_11.pdfBluetongue virusCulicoidesEpizootic haemorrhagic disease virusITS-1ITS-2Slovakia |
| spellingShingle | Adela Sarvašová Maria Goffredo Igor Sopoliga Giovanni Savini Alica Kočišová Culicoides midges (Diptera: Ceratopogonidae) as vectors of orbiviruses in Slovakia Veterinaria Italiana Bluetongue virus Culicoides Epizootic haemorrhagic disease virus ITS-1 ITS-2 Slovakia |
| title | Culicoides midges (Diptera: Ceratopogonidae) as vectors of orbiviruses in Slovakia |
| title_full | Culicoides midges (Diptera: Ceratopogonidae) as vectors of orbiviruses in Slovakia |
| title_fullStr | Culicoides midges (Diptera: Ceratopogonidae) as vectors of orbiviruses in Slovakia |
| title_full_unstemmed | Culicoides midges (Diptera: Ceratopogonidae) as vectors of orbiviruses in Slovakia |
| title_short | Culicoides midges (Diptera: Ceratopogonidae) as vectors of orbiviruses in Slovakia |
| title_sort | culicoides midges diptera ceratopogonidae as vectors of orbiviruses in slovakia |
| topic | Bluetongue virus Culicoides Epizootic haemorrhagic disease virus ITS-1 ITS-2 Slovakia |
| url | http://www.izs.it/vet_italiana/2014/50_3/VetIt_2405_11.pdf |
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