Not Just a Sum? Identifying Different Types of Interplay between Constituents in Combined Interventions.
<h4>Motivation</h4>Experiments in which the effect of combined manipulations is compared with the effects of their pure constituents have received a great deal of attention. Examples include the study of combination therapies and the comparison of double and single knockout model organis...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0125334 |
| _version_ | 1818359734341730304 |
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| author | Katrijn Van Deun Lieven Thorrez Robert A van den Berg Age K Smilde Iven Van Mechelen |
| author_facet | Katrijn Van Deun Lieven Thorrez Robert A van den Berg Age K Smilde Iven Van Mechelen |
| author_sort | Katrijn Van Deun |
| collection | DOAJ |
| description | <h4>Motivation</h4>Experiments in which the effect of combined manipulations is compared with the effects of their pure constituents have received a great deal of attention. Examples include the study of combination therapies and the comparison of double and single knockout model organisms. Often the effect of the combined manipulation is not a mere addition of the effects of its constituents, with quite different forms of interplay between the constituents being possible. Yet, a well-formalized taxonomy of possible forms of interplay is lacking, let alone a statistical methodology to test for their presence in empirical data.<h4>Results</h4>Starting from a taxonomy of a broad range of forms of interplay between constituents of a combined manipulation, we propose a sound statistical hypothesis testing framework to test for the presence of each particular form of interplay. We illustrate the framework with analyses of public gene expression data on the combined treatment of dendritic cells with curdlan and GM-CSF and show that these lead to valuable insights into the mode of action of the constituent treatments and their combination.<h4>Availability and implementation</h4>R code implementing the statistical testing procedure for microarray gene expression data is available as supplementary material. The data are available from the Gene Expression Omnibus with accession number GSE32986. |
| first_indexed | 2024-12-13T20:49:36Z |
| format | Article |
| id | doaj.art-08d8cf95b2154f76abe1df03c17bdf4e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T20:49:36Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-08d8cf95b2154f76abe1df03c17bdf4e2022-12-21T23:31:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012533410.1371/journal.pone.0125334Not Just a Sum? Identifying Different Types of Interplay between Constituents in Combined Interventions.Katrijn Van DeunLieven ThorrezRobert A van den BergAge K SmildeIven Van Mechelen<h4>Motivation</h4>Experiments in which the effect of combined manipulations is compared with the effects of their pure constituents have received a great deal of attention. Examples include the study of combination therapies and the comparison of double and single knockout model organisms. Often the effect of the combined manipulation is not a mere addition of the effects of its constituents, with quite different forms of interplay between the constituents being possible. Yet, a well-formalized taxonomy of possible forms of interplay is lacking, let alone a statistical methodology to test for their presence in empirical data.<h4>Results</h4>Starting from a taxonomy of a broad range of forms of interplay between constituents of a combined manipulation, we propose a sound statistical hypothesis testing framework to test for the presence of each particular form of interplay. We illustrate the framework with analyses of public gene expression data on the combined treatment of dendritic cells with curdlan and GM-CSF and show that these lead to valuable insights into the mode of action of the constituent treatments and their combination.<h4>Availability and implementation</h4>R code implementing the statistical testing procedure for microarray gene expression data is available as supplementary material. The data are available from the Gene Expression Omnibus with accession number GSE32986.https://doi.org/10.1371/journal.pone.0125334 |
| spellingShingle | Katrijn Van Deun Lieven Thorrez Robert A van den Berg Age K Smilde Iven Van Mechelen Not Just a Sum? Identifying Different Types of Interplay between Constituents in Combined Interventions. PLoS ONE |
| title | Not Just a Sum? Identifying Different Types of Interplay between Constituents in Combined Interventions. |
| title_full | Not Just a Sum? Identifying Different Types of Interplay between Constituents in Combined Interventions. |
| title_fullStr | Not Just a Sum? Identifying Different Types of Interplay between Constituents in Combined Interventions. |
| title_full_unstemmed | Not Just a Sum? Identifying Different Types of Interplay between Constituents in Combined Interventions. |
| title_short | Not Just a Sum? Identifying Different Types of Interplay between Constituents in Combined Interventions. |
| title_sort | not just a sum identifying different types of interplay between constituents in combined interventions |
| url | https://doi.org/10.1371/journal.pone.0125334 |
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