Ability of <it>Lactobacillus fermentum </it>to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides
<p>Abstract</p> <p>Background</p> <p>Soya and its derivatives represent nutritionally high quality food products whose major drawback is their high content of α-galacto-oligosaccharides. These are not digested in the small intestine due to the natural absence of tissula...
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| Format: | Article |
| Language: | English |
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BMC
2008-01-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/8/22 |
| _version_ | 1818049906746589184 |
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| author | Sesma Fernando Guerekobaya Theodora de Giori Graciela Bensaada Martine Ledue-Clier Florence LeBlanc Jean Juillard Vincent Rabot Sylvie Piard Jean-Christophe |
| author_facet | Sesma Fernando Guerekobaya Theodora de Giori Graciela Bensaada Martine Ledue-Clier Florence LeBlanc Jean Juillard Vincent Rabot Sylvie Piard Jean-Christophe |
| author_sort | Sesma Fernando |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Soya and its derivatives represent nutritionally high quality food products whose major drawback is their high content of α-galacto-oligosaccharides. These are not digested in the small intestine due to the natural absence of tissular α-galactosidase in mammals. The passage of these carbohydrates to the large intestine makes them available for fermentation by gas-producing bacteria leading to intestinal flatulence. The aim of the work reported here was to assess the ability of α-galactosidase-producing lactobacilli to improve the digestibility of α-galacto-oligosaccharides <it>in situ</it>.</p> <p>Results</p> <p>Gnotobiotic rats were orally fed with soy milk and placed in respiratory chambers designed to monitor fermentative gas excretion. The validity of the animal model was first checked using gnotobiotic rats monoassociated with a <it>Clostridium butyricum </it>hydrogen (H<sub>2</sub>)-producing strain. Ingestion of native soy milk by these rats caused significant H<sub>2 </sub>emission while ingestion of α-galacto-oligosaccharide-free soy milk did not, thus validating the experimental system. When native soy milk was fermented using the α-galactosidase-producing <it>Lactobacillus fermentum </it>CRL722 strain, the resulting product failed to induce H<sub>2 </sub>emission in rats thus validating the bacterial model. When <it>L. fermentum </it>CRL722 was coadministered with native soy milk, a significant reduction (50 %, <it>P </it>= 0.019) in H<sub>2 </sub>emission was observed, showing that α-galactosidase from <it>L. fermentum </it>CRL722 remained active <it>in situ</it>, in the gastrointestinal tract of rats monoassociated with <it>C. butyricum</it>. In human-microbiota associated rats, <it>L. fermentum </it>CRL722 also induced a significant reduction of H<sub>2 </sub>emission (70 %, <it>P </it>= 0.004).</p> <p>Conclusion</p> <p>These results strongly suggest that <it>L. fermentum </it>α-galactosidase is able to partially alleviate α-galactosidase deficiency in rats. This offers interesting perspectives in various applications in which lactic acid bacteria could be used as a vector for delivery of digestive enzymes in man and animals.</p> |
| first_indexed | 2024-12-10T10:45:01Z |
| format | Article |
| id | doaj.art-08dc4edba6cb48419cd8aff66dbd9f82 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-10T10:45:01Z |
| publishDate | 2008-01-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj.art-08dc4edba6cb48419cd8aff66dbd9f822022-12-22T01:52:11ZengBMCBMC Microbiology1471-21802008-01-01812210.1186/1471-2180-8-22Ability of <it>Lactobacillus fermentum </it>to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharidesSesma FernandoGuerekobaya Theodorade Giori GracielaBensaada MartineLedue-Clier FlorenceLeBlanc JeanJuillard VincentRabot SylviePiard Jean-Christophe<p>Abstract</p> <p>Background</p> <p>Soya and its derivatives represent nutritionally high quality food products whose major drawback is their high content of α-galacto-oligosaccharides. These are not digested in the small intestine due to the natural absence of tissular α-galactosidase in mammals. The passage of these carbohydrates to the large intestine makes them available for fermentation by gas-producing bacteria leading to intestinal flatulence. The aim of the work reported here was to assess the ability of α-galactosidase-producing lactobacilli to improve the digestibility of α-galacto-oligosaccharides <it>in situ</it>.</p> <p>Results</p> <p>Gnotobiotic rats were orally fed with soy milk and placed in respiratory chambers designed to monitor fermentative gas excretion. The validity of the animal model was first checked using gnotobiotic rats monoassociated with a <it>Clostridium butyricum </it>hydrogen (H<sub>2</sub>)-producing strain. Ingestion of native soy milk by these rats caused significant H<sub>2 </sub>emission while ingestion of α-galacto-oligosaccharide-free soy milk did not, thus validating the experimental system. When native soy milk was fermented using the α-galactosidase-producing <it>Lactobacillus fermentum </it>CRL722 strain, the resulting product failed to induce H<sub>2 </sub>emission in rats thus validating the bacterial model. When <it>L. fermentum </it>CRL722 was coadministered with native soy milk, a significant reduction (50 %, <it>P </it>= 0.019) in H<sub>2 </sub>emission was observed, showing that α-galactosidase from <it>L. fermentum </it>CRL722 remained active <it>in situ</it>, in the gastrointestinal tract of rats monoassociated with <it>C. butyricum</it>. In human-microbiota associated rats, <it>L. fermentum </it>CRL722 also induced a significant reduction of H<sub>2 </sub>emission (70 %, <it>P </it>= 0.004).</p> <p>Conclusion</p> <p>These results strongly suggest that <it>L. fermentum </it>α-galactosidase is able to partially alleviate α-galactosidase deficiency in rats. This offers interesting perspectives in various applications in which lactic acid bacteria could be used as a vector for delivery of digestive enzymes in man and animals.</p>http://www.biomedcentral.com/1471-2180/8/22 |
| spellingShingle | Sesma Fernando Guerekobaya Theodora de Giori Graciela Bensaada Martine Ledue-Clier Florence LeBlanc Jean Juillard Vincent Rabot Sylvie Piard Jean-Christophe Ability of <it>Lactobacillus fermentum </it>to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides BMC Microbiology |
| title | Ability of <it>Lactobacillus fermentum </it>to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides |
| title_full | Ability of <it>Lactobacillus fermentum </it>to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides |
| title_fullStr | Ability of <it>Lactobacillus fermentum </it>to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides |
| title_full_unstemmed | Ability of <it>Lactobacillus fermentum </it>to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides |
| title_short | Ability of <it>Lactobacillus fermentum </it>to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides |
| title_sort | ability of it lactobacillus fermentum it to overcome host α galactosidase deficiency as evidenced by reduction of hydrogen excretion in rats consuming soya α galacto oligosaccharides |
| url | http://www.biomedcentral.com/1471-2180/8/22 |
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