Antibacterial Activity of ZnSe, ZnSe-TiO<sub>2</sub> and TiO<sub>2</sub> Particles Tailored by Lysozyme Loading and Visible Light Irradiation
ZnSe, ZnSe-TiO<sub>2</sub> microspheres and nanostructured TiO<sub>2</sub> obtained by hydrothermal and sol–gel methods were tested against <i>Staphylococcus aureus</i> ATCC 25923 and <i>Micrococcus lysodeikticus</i> ATCC 4698 before and after lysozyme...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/3/691 |
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| author | Crina Anastasescu Simona Neagu Silviu Preda Daniela Culita Mihaela Stancu Cristian Banciu Cornel Munteanu Veronica Bratan Jose Maria Calderon-Moreno Razvan State Mihai Anastasescu Madalin Enache Ioan Balint Maria Zaharescu |
| author_facet | Crina Anastasescu Simona Neagu Silviu Preda Daniela Culita Mihaela Stancu Cristian Banciu Cornel Munteanu Veronica Bratan Jose Maria Calderon-Moreno Razvan State Mihai Anastasescu Madalin Enache Ioan Balint Maria Zaharescu |
| author_sort | Crina Anastasescu |
| collection | DOAJ |
| description | ZnSe, ZnSe-TiO<sub>2</sub> microspheres and nanostructured TiO<sub>2</sub> obtained by hydrothermal and sol–gel methods were tested against <i>Staphylococcus aureus</i> ATCC 25923 and <i>Micrococcus lysodeikticus</i> ATCC 4698 before and after lysozyme (Lys) loading. Morphological characterization of inorganic matrices and hybrid organic–inorganic complexes were performed by microscopy techniques (SEM, AFM and Dark Field Hyperspectral Microscopy). Light absorption properties of ZnSe, ZnSe-TiO<sub>2</sub> and TiO<sub>2</sub> powders were assessed by UV–visible spectroscopy and their ability to generate reactive oxygen species (•OH and O<sub>2</sub><sup>•−</sup>) under visible light irradiation was investigated. Antibacterial activity of ZnSe, ZnSe-TiO<sub>2</sub>, TiO<sub>2</sub>, Lys/ZnSe, Lys/ZnSe-TiO<sub>2</sub> and Lys/TiO<sub>2</sub> samples under exposure to visible light irradiation (λ > 420 nm) was tested against <i>Staphylococcus aureus</i> and <i>Micrococcus lysodeikticus</i> and correlated with ROS photogeneration. |
| first_indexed | 2024-03-11T07:00:08Z |
| format | Article |
| id | doaj.art-08dcf4590801467a9683188bd738dfa5 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T07:00:08Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-08dcf4590801467a9683188bd738dfa52023-11-17T09:18:25ZengMDPI AGAntioxidants2076-39212023-03-0112369110.3390/antiox12030691Antibacterial Activity of ZnSe, ZnSe-TiO<sub>2</sub> and TiO<sub>2</sub> Particles Tailored by Lysozyme Loading and Visible Light IrradiationCrina Anastasescu0Simona Neagu1Silviu Preda2Daniela Culita3Mihaela Stancu4Cristian Banciu5Cornel Munteanu6Veronica Bratan7Jose Maria Calderon-Moreno8Razvan State9Mihai Anastasescu10Madalin Enache11Ioan Balint12Maria Zaharescu13“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaInstitute of Biology of Romanian Academy, 296 Splaiul Independenţei, 060031 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaInstitute of Biology of Romanian Academy, 296 Splaiul Independenţei, 060031 Bucharest, RomaniaInstitute of Biology of Romanian Academy, 296 Splaiul Independenţei, 060031 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaInstitute of Biology of Romanian Academy, 296 Splaiul Independenţei, 060031 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaZnSe, ZnSe-TiO<sub>2</sub> microspheres and nanostructured TiO<sub>2</sub> obtained by hydrothermal and sol–gel methods were tested against <i>Staphylococcus aureus</i> ATCC 25923 and <i>Micrococcus lysodeikticus</i> ATCC 4698 before and after lysozyme (Lys) loading. Morphological characterization of inorganic matrices and hybrid organic–inorganic complexes were performed by microscopy techniques (SEM, AFM and Dark Field Hyperspectral Microscopy). Light absorption properties of ZnSe, ZnSe-TiO<sub>2</sub> and TiO<sub>2</sub> powders were assessed by UV–visible spectroscopy and their ability to generate reactive oxygen species (•OH and O<sub>2</sub><sup>•−</sup>) under visible light irradiation was investigated. Antibacterial activity of ZnSe, ZnSe-TiO<sub>2</sub>, TiO<sub>2</sub>, Lys/ZnSe, Lys/ZnSe-TiO<sub>2</sub> and Lys/TiO<sub>2</sub> samples under exposure to visible light irradiation (λ > 420 nm) was tested against <i>Staphylococcus aureus</i> and <i>Micrococcus lysodeikticus</i> and correlated with ROS photogeneration.https://www.mdpi.com/2076-3921/12/3/691ZnSeZnSe-TiO<sub>2</sub>TiO<sub>2</sub>hybrid organic–inorganiclight absorption propertiesreactive oxygen species generation |
| spellingShingle | Crina Anastasescu Simona Neagu Silviu Preda Daniela Culita Mihaela Stancu Cristian Banciu Cornel Munteanu Veronica Bratan Jose Maria Calderon-Moreno Razvan State Mihai Anastasescu Madalin Enache Ioan Balint Maria Zaharescu Antibacterial Activity of ZnSe, ZnSe-TiO<sub>2</sub> and TiO<sub>2</sub> Particles Tailored by Lysozyme Loading and Visible Light Irradiation Antioxidants ZnSe ZnSe-TiO<sub>2</sub> TiO<sub>2</sub> hybrid organic–inorganic light absorption properties reactive oxygen species generation |
| title | Antibacterial Activity of ZnSe, ZnSe-TiO<sub>2</sub> and TiO<sub>2</sub> Particles Tailored by Lysozyme Loading and Visible Light Irradiation |
| title_full | Antibacterial Activity of ZnSe, ZnSe-TiO<sub>2</sub> and TiO<sub>2</sub> Particles Tailored by Lysozyme Loading and Visible Light Irradiation |
| title_fullStr | Antibacterial Activity of ZnSe, ZnSe-TiO<sub>2</sub> and TiO<sub>2</sub> Particles Tailored by Lysozyme Loading and Visible Light Irradiation |
| title_full_unstemmed | Antibacterial Activity of ZnSe, ZnSe-TiO<sub>2</sub> and TiO<sub>2</sub> Particles Tailored by Lysozyme Loading and Visible Light Irradiation |
| title_short | Antibacterial Activity of ZnSe, ZnSe-TiO<sub>2</sub> and TiO<sub>2</sub> Particles Tailored by Lysozyme Loading and Visible Light Irradiation |
| title_sort | antibacterial activity of znse znse tio sub 2 sub and tio sub 2 sub particles tailored by lysozyme loading and visible light irradiation |
| topic | ZnSe ZnSe-TiO<sub>2</sub> TiO<sub>2</sub> hybrid organic–inorganic light absorption properties reactive oxygen species generation |
| url | https://www.mdpi.com/2076-3921/12/3/691 |
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