A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase.
A carbapenem-resistant strain of Pseudomonas aeruginosa, NCGM1984, was isolated in 2012 from a hospitalized patient in Japan. Immunochromatographic assay showed that the isolate was positive for IMP-type metallo-β-lactamase. Complete genome sequencing revealed that NCGM1984 harbored two copies of bl...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4824433?pdf=render |
| _version_ | 1811214375273365504 |
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| author | Tatsuya Tada Tohru Miyoshi-Akiyama Kayo Shimada Akino Shiroma Kazuma Nakano Kuniko Teruya Kazuhito Satou Takashi Hirano Masahiro Shimojima Teruo Kirikae |
| author_facet | Tatsuya Tada Tohru Miyoshi-Akiyama Kayo Shimada Akino Shiroma Kazuma Nakano Kuniko Teruya Kazuhito Satou Takashi Hirano Masahiro Shimojima Teruo Kirikae |
| author_sort | Tatsuya Tada |
| collection | DOAJ |
| description | A carbapenem-resistant strain of Pseudomonas aeruginosa, NCGM1984, was isolated in 2012 from a hospitalized patient in Japan. Immunochromatographic assay showed that the isolate was positive for IMP-type metallo-β-lactamase. Complete genome sequencing revealed that NCGM1984 harbored two copies of blaIMP-34, located at different sites on the chromosome. Each blaIMP-34 was present in the same structures of the class 1 integrons, tnpA(ISPa7)-intI1-qacG-blaIMP-34-aac(6')-Ib-qacEdelta1-sul1-orf5-tniBdelta-tniA. The isolate belonged to multilocus sequence typing ST235, one of the international high-risk clones. IMP-34, with an amino acid substitution (Glu126Gly) compared with IMP-1, hydrolyzed all β-lactamases tested except aztreonam, and its catalytic activities were similar to IMP-1. This is the first report of a clinical isolate of an IMP-34-producing P. aeruginosa harboring two copies of blaIMP-34 on its chromosome. |
| first_indexed | 2024-04-12T06:03:23Z |
| format | Article |
| id | doaj.art-08dde866219b4f29a10982f15e41c6b1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T06:03:23Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-08dde866219b4f29a10982f15e41c6b12022-12-22T03:44:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01114e014938510.1371/journal.pone.0149385A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase.Tatsuya TadaTohru Miyoshi-AkiyamaKayo ShimadaAkino ShiromaKazuma NakanoKuniko TeruyaKazuhito SatouTakashi HiranoMasahiro ShimojimaTeruo KirikaeA carbapenem-resistant strain of Pseudomonas aeruginosa, NCGM1984, was isolated in 2012 from a hospitalized patient in Japan. Immunochromatographic assay showed that the isolate was positive for IMP-type metallo-β-lactamase. Complete genome sequencing revealed that NCGM1984 harbored two copies of blaIMP-34, located at different sites on the chromosome. Each blaIMP-34 was present in the same structures of the class 1 integrons, tnpA(ISPa7)-intI1-qacG-blaIMP-34-aac(6')-Ib-qacEdelta1-sul1-orf5-tniBdelta-tniA. The isolate belonged to multilocus sequence typing ST235, one of the international high-risk clones. IMP-34, with an amino acid substitution (Glu126Gly) compared with IMP-1, hydrolyzed all β-lactamases tested except aztreonam, and its catalytic activities were similar to IMP-1. This is the first report of a clinical isolate of an IMP-34-producing P. aeruginosa harboring two copies of blaIMP-34 on its chromosome.http://europepmc.org/articles/PMC4824433?pdf=render |
| spellingShingle | Tatsuya Tada Tohru Miyoshi-Akiyama Kayo Shimada Akino Shiroma Kazuma Nakano Kuniko Teruya Kazuhito Satou Takashi Hirano Masahiro Shimojima Teruo Kirikae A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase. PLoS ONE |
| title | A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase. |
| title_full | A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase. |
| title_fullStr | A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase. |
| title_full_unstemmed | A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase. |
| title_short | A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase. |
| title_sort | carbapenem resistant pseudomonas aeruginosa isolate harboring two copies of blaimp 34 encoding a metallo β lactamase |
| url | http://europepmc.org/articles/PMC4824433?pdf=render |
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