Identification of SbWRKY Transcription Factors in <i>Scutellaria baicalensis</i> Georgi under Drought Stress and Their Relationship with Baicalin
One of the most significant transcription factors in plants, <i>WRKYs,</i> are crucial for plant growth and stress response. In this study, we analyzed the physicochemical properties, evolutionary relationships, conservation structure, and expression of the WRKY gene family in <i>S...
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MDPI AG
2023-10-01
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| Series: | Agronomy |
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| author | Lin Cheng Jingjing Yu Lichao Zhang Yanying Yao Zhuo Sun Mei Han Yonggang Zhang Limin Yang |
| author_facet | Lin Cheng Jingjing Yu Lichao Zhang Yanying Yao Zhuo Sun Mei Han Yonggang Zhang Limin Yang |
| author_sort | Lin Cheng |
| collection | DOAJ |
| description | One of the most significant transcription factors in plants, <i>WRKYs,</i> are crucial for plant growth and stress response. In this study, we analyzed the physicochemical properties, evolutionary relationships, conservation structure, and expression of the WRKY gene family in <i>S. baicalensis.</i> The WRKY family has highly conserved structural domains, which have been classified into three major categories, I, II, and III, based on the number of WRKY structural domains and zinc finger structural features. <i>SbWRKYs</i> of the same subgroup are functionally similar and essentially contain the same motif. Additionally, different drought stress situations resulted in varying levels of <i>SbWRKYs</i> expression, with the majority of these factors being up-regulated in moderate drought stress settings, and fewer of them were up-regulated under severe drought stress conditions. Under moderate drought stress, the expression of key enzymes increased, while under severe drought stress, the expression of key enzymes decreased. Mild drought stress resulted in a 26.42% increase in baicalin accumulation, while severe drought stress led to a 22.88% decrease. The protein interaction analysis of key enzyme genes and <i>SbWRKYs</i> revealed that the expression of key enzyme genes affected the expression of <i>SbWRKYs</i>. We screened nine <i>SbWRKYs</i> with a significant relationship with baicalin accumulation, and SbWRKY8 and SbWRKY16 showed the highest correlation with the baicalin content. These findings offer a theoretical framework for more research on the roles of <i>SbWRKYs</i> and show that <i>SbWRKYs</i> can respond to drought stress in <i>S. baicalensis</i>. |
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| spelling | doaj.art-08dfeb2d6d5c426b874a14ae2d28eb492023-11-19T15:21:57ZengMDPI AGAgronomy2073-43952023-10-011310256410.3390/agronomy13102564Identification of SbWRKY Transcription Factors in <i>Scutellaria baicalensis</i> Georgi under Drought Stress and Their Relationship with BaicalinLin Cheng0Jingjing Yu1Lichao Zhang2Yanying Yao3Zhuo Sun4Mei Han5Yonggang Zhang6Limin Yang7Cultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management of Jilin Province and Ministry of Science and Technology, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management of Jilin Province and Ministry of Science and Technology, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaTechnology Service Center on Ecological Planting of Chinese Herbal Medicine in Chengde, Chengde 067000, ChinaGeneral Station of Agricultural Environmental Protection and Rural Energy Management of Jilin Province, Changchun 130031, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management of Jilin Province and Ministry of Science and Technology, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management of Jilin Province and Ministry of Science and Technology, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management of Jilin Province and Ministry of Science and Technology, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management of Jilin Province and Ministry of Science and Technology, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaOne of the most significant transcription factors in plants, <i>WRKYs,</i> are crucial for plant growth and stress response. In this study, we analyzed the physicochemical properties, evolutionary relationships, conservation structure, and expression of the WRKY gene family in <i>S. baicalensis.</i> The WRKY family has highly conserved structural domains, which have been classified into three major categories, I, II, and III, based on the number of WRKY structural domains and zinc finger structural features. <i>SbWRKYs</i> of the same subgroup are functionally similar and essentially contain the same motif. Additionally, different drought stress situations resulted in varying levels of <i>SbWRKYs</i> expression, with the majority of these factors being up-regulated in moderate drought stress settings, and fewer of them were up-regulated under severe drought stress conditions. Under moderate drought stress, the expression of key enzymes increased, while under severe drought stress, the expression of key enzymes decreased. Mild drought stress resulted in a 26.42% increase in baicalin accumulation, while severe drought stress led to a 22.88% decrease. The protein interaction analysis of key enzyme genes and <i>SbWRKYs</i> revealed that the expression of key enzyme genes affected the expression of <i>SbWRKYs</i>. We screened nine <i>SbWRKYs</i> with a significant relationship with baicalin accumulation, and SbWRKY8 and SbWRKY16 showed the highest correlation with the baicalin content. These findings offer a theoretical framework for more research on the roles of <i>SbWRKYs</i> and show that <i>SbWRKYs</i> can respond to drought stress in <i>S. baicalensis</i>.https://www.mdpi.com/2073-4395/13/10/2564<i>Scutellaria baicalensis</i> GeorgiSbWRKYsdrought stresskey enzyme genebaicalincorrelation |
| spellingShingle | Lin Cheng Jingjing Yu Lichao Zhang Yanying Yao Zhuo Sun Mei Han Yonggang Zhang Limin Yang Identification of SbWRKY Transcription Factors in <i>Scutellaria baicalensis</i> Georgi under Drought Stress and Their Relationship with Baicalin Agronomy <i>Scutellaria baicalensis</i> Georgi SbWRKYs drought stress key enzyme gene baicalin correlation |
| title | Identification of SbWRKY Transcription Factors in <i>Scutellaria baicalensis</i> Georgi under Drought Stress and Their Relationship with Baicalin |
| title_full | Identification of SbWRKY Transcription Factors in <i>Scutellaria baicalensis</i> Georgi under Drought Stress and Their Relationship with Baicalin |
| title_fullStr | Identification of SbWRKY Transcription Factors in <i>Scutellaria baicalensis</i> Georgi under Drought Stress and Their Relationship with Baicalin |
| title_full_unstemmed | Identification of SbWRKY Transcription Factors in <i>Scutellaria baicalensis</i> Georgi under Drought Stress and Their Relationship with Baicalin |
| title_short | Identification of SbWRKY Transcription Factors in <i>Scutellaria baicalensis</i> Georgi under Drought Stress and Their Relationship with Baicalin |
| title_sort | identification of sbwrky transcription factors in i scutellaria baicalensis i georgi under drought stress and their relationship with baicalin |
| topic | <i>Scutellaria baicalensis</i> Georgi SbWRKYs drought stress key enzyme gene baicalin correlation |
| url | https://www.mdpi.com/2073-4395/13/10/2564 |
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