Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum infection
INTRODUCTION: During histoplasmosis, Histoplasma capsulatum soluble antigens (CFAg) can be naturally released by yeast cells. Because CFAg can be specifically targeted during infection, in the present study we investigated CFAg release in experimental murine histoplasmosis, and evaluated the host hu...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2012-04-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000200018&lng=en&tlng=en |
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| author | Fabrine Sales Massafera Tristão Paula César Leonello Luciene Airy Nagashima Ayako Sano Mário Augusto Ono Eiko Nakagawa Itano |
| author_facet | Fabrine Sales Massafera Tristão Paula César Leonello Luciene Airy Nagashima Ayako Sano Mário Augusto Ono Eiko Nakagawa Itano |
| author_sort | Fabrine Sales Massafera Tristão |
| collection | DOAJ |
| description | INTRODUCTION: During histoplasmosis, Histoplasma capsulatum soluble antigens (CFAg) can be naturally released by yeast cells. Because CFAg can be specifically targeted during infection, in the present study we investigated CFAg release in experimental murine histoplasmosis, and evaluated the host humoral immune response against high-molecular-mass antigens (hMMAg. >150 kDa), the more immunogenic CFAg fraction. METHODS: Mice were infected with 2.2x10(4) H. capsulatum IMT/HC128 yeast cells. The soluble CFAg, IgG anti-CFAg, IgG anti-hMMAg, and IgG-hMMAg circulating immune complexes (CIC) levels were determined by enzymelinked immunosorbent assay, at days 0, 7, 14, and 28 post-infection. RESULTS: We observed a progressive increase in circulating levels of CFAg, IgG anti-CFAg, IgG anti-hMMAg, and IgG-hMMAg CIC after H. capsulatum infection. The hMMAg showed a high percentage of carbohydrates and at least two main immunogenic components. CONCLUSIONS: We verified for the first time that hMMAg from H. capsulatum IMT/HC128 strain induce humoral immune response and lead to CIC formation during experimental histoplasmosis. |
| first_indexed | 2024-04-13T22:58:16Z |
| format | Article |
| id | doaj.art-08e1ba4c803e421ea4688dd020aea4f6 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-04-13T22:58:16Z |
| publishDate | 2012-04-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-08e1ba4c803e421ea4688dd020aea4f62022-12-22T02:25:56ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492012-04-0145223223710.1590/S0037-86822012000200018S0037-86822012000200018Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum infectionFabrine Sales Massafera Tristão0Paula César Leonello1Luciene Airy Nagashima2Ayako Sano3Mário Augusto Ono4Eiko Nakagawa Itano5Universidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaChiba UniversityUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaINTRODUCTION: During histoplasmosis, Histoplasma capsulatum soluble antigens (CFAg) can be naturally released by yeast cells. Because CFAg can be specifically targeted during infection, in the present study we investigated CFAg release in experimental murine histoplasmosis, and evaluated the host humoral immune response against high-molecular-mass antigens (hMMAg. >150 kDa), the more immunogenic CFAg fraction. METHODS: Mice were infected with 2.2x10(4) H. capsulatum IMT/HC128 yeast cells. The soluble CFAg, IgG anti-CFAg, IgG anti-hMMAg, and IgG-hMMAg circulating immune complexes (CIC) levels were determined by enzymelinked immunosorbent assay, at days 0, 7, 14, and 28 post-infection. RESULTS: We observed a progressive increase in circulating levels of CFAg, IgG anti-CFAg, IgG anti-hMMAg, and IgG-hMMAg CIC after H. capsulatum infection. The hMMAg showed a high percentage of carbohydrates and at least two main immunogenic components. CONCLUSIONS: We verified for the first time that hMMAg from H. capsulatum IMT/HC128 strain induce humoral immune response and lead to CIC formation during experimental histoplasmosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000200018&lng=en&tlng=enAntígenos solúveisHistoplasmoseImunocomplexos |
| spellingShingle | Fabrine Sales Massafera Tristão Paula César Leonello Luciene Airy Nagashima Ayako Sano Mário Augusto Ono Eiko Nakagawa Itano Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum infection Revista da Sociedade Brasileira de Medicina Tropical Antígenos solúveis Histoplasmose Imunocomplexos |
| title | Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum infection |
| title_full | Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum infection |
| title_fullStr | Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum infection |
| title_full_unstemmed | Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum infection |
| title_short | Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum infection |
| title_sort | carbohydrate rich high molecular mass antigens are strongly recognized during experimental histoplasma capsulatum infection |
| topic | Antígenos solúveis Histoplasmose Imunocomplexos |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000200018&lng=en&tlng=en |
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