A novel cytochrome P450 gene from Catharanthus roseus cell line C20hi: cloning and characterization of expression
An expressed sequence tag (EST) obtained from a subtractive-suppression hybridization cDNA library constructed using Catharanthus roseus cell line C20hi and its parental cell line C20D was used to clone a full-length cytochrome P450 cDNA of cyp71d1. The encoded polypeptide contained 507 amino acids...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2012-06-01
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| Series: | Acta Pharmaceutica Sinica B |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211383512000275 |
| _version_ | 1811264429689405440 |
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| author | Lihong He Shujuan Zhao Zhibi Hu |
| author_facet | Lihong He Shujuan Zhao Zhibi Hu |
| author_sort | Lihong He |
| collection | DOAJ |
| description | An expressed sequence tag (EST) obtained from a subtractive-suppression hybridization cDNA library constructed using Catharanthus roseus cell line C20hi and its parental cell line C20D was used to clone a full-length cytochrome P450 cDNA of cyp71d1. The encoded polypeptide contained 507 amino acids with 39–56% identity to other CYP71D subfamily members at the amino acid level. Expression characteristics of cyp71d1 were determined using semi-quantitative RT-PCR. The cyp71d1 transcript was expressed in all three cell lines with the highest level in the cell line C20hi. In the mature C. roseus plant, the cyp71d1 cDNA was highly expressed in petals, roots and stems, but very weakly expressed in young leaves. Its transcription level increased with the development of flowers. 2,4-D could down-regulate the transcription of cyp71d1, as did KT, but only to a minor degree. Neither light nor yeast elicitor could induce the transcription of cyp71d1. |
| first_indexed | 2024-04-12T20:03:01Z |
| format | Article |
| id | doaj.art-08eae688696b4154ae2df091c2f98b69 |
| institution | Directory Open Access Journal |
| issn | 2211-3835 2211-3843 |
| language | English |
| last_indexed | 2024-04-12T20:03:01Z |
| publishDate | 2012-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj.art-08eae688696b4154ae2df091c2f98b692022-12-22T03:18:28ZengElsevierActa Pharmaceutica Sinica B2211-38352211-38432012-06-012325025510.1016/j.apsb.2012.01.006A novel cytochrome P450 gene from Catharanthus roseus cell line C20hi: cloning and characterization of expressionLihong HeShujuan ZhaoZhibi HuAn expressed sequence tag (EST) obtained from a subtractive-suppression hybridization cDNA library constructed using Catharanthus roseus cell line C20hi and its parental cell line C20D was used to clone a full-length cytochrome P450 cDNA of cyp71d1. The encoded polypeptide contained 507 amino acids with 39–56% identity to other CYP71D subfamily members at the amino acid level. Expression characteristics of cyp71d1 were determined using semi-quantitative RT-PCR. The cyp71d1 transcript was expressed in all three cell lines with the highest level in the cell line C20hi. In the mature C. roseus plant, the cyp71d1 cDNA was highly expressed in petals, roots and stems, but very weakly expressed in young leaves. Its transcription level increased with the development of flowers. 2,4-D could down-regulate the transcription of cyp71d1, as did KT, but only to a minor degree. Neither light nor yeast elicitor could induce the transcription of cyp71d1.http://www.sciencedirect.com/science/article/pii/S2211383512000275Cytochrome P450cyp71d1TranscriptionTissue specific expressionFull habituated cell line |
| spellingShingle | Lihong He Shujuan Zhao Zhibi Hu A novel cytochrome P450 gene from Catharanthus roseus cell line C20hi: cloning and characterization of expression Acta Pharmaceutica Sinica B Cytochrome P450 cyp71d1 Transcription Tissue specific expression Full habituated cell line |
| title | A novel cytochrome P450 gene from Catharanthus roseus cell line C20hi: cloning and characterization of expression |
| title_full | A novel cytochrome P450 gene from Catharanthus roseus cell line C20hi: cloning and characterization of expression |
| title_fullStr | A novel cytochrome P450 gene from Catharanthus roseus cell line C20hi: cloning and characterization of expression |
| title_full_unstemmed | A novel cytochrome P450 gene from Catharanthus roseus cell line C20hi: cloning and characterization of expression |
| title_short | A novel cytochrome P450 gene from Catharanthus roseus cell line C20hi: cloning and characterization of expression |
| title_sort | novel cytochrome p450 gene from catharanthus roseus cell line c20hi cloning and characterization of expression |
| topic | Cytochrome P450 cyp71d1 Transcription Tissue specific expression Full habituated cell line |
| url | http://www.sciencedirect.com/science/article/pii/S2211383512000275 |
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