Application status and optimization suggestions of tumor organoids and CAR-T cell co-culture models
Abstract Tumor organoids, especially patient-derived organoids (PDOs) exhibit marked similarities in histopathological morphology, genomic alterations, and specific marker expression profiles to those of primary tumour tissues. They are applied in various fields including drug screening, gene editin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-024-03272-x |
| _version_ | 1797266541740294144 |
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| author | Rong-Xuan Ning Cun-Yu Liu Shi-Qi Wang Wen-Kai Li Xia Kong Zhi-Wei He |
| author_facet | Rong-Xuan Ning Cun-Yu Liu Shi-Qi Wang Wen-Kai Li Xia Kong Zhi-Wei He |
| author_sort | Rong-Xuan Ning |
| collection | DOAJ |
| description | Abstract Tumor organoids, especially patient-derived organoids (PDOs) exhibit marked similarities in histopathological morphology, genomic alterations, and specific marker expression profiles to those of primary tumour tissues. They are applied in various fields including drug screening, gene editing, and identification of oncogenes. However, CAR-T therapy in the treatment of solid tumours is still at an exploratory stage. Tumour organoids offer unique advantages over other preclinical models commonly used for CAR-T therapy research, which the preservation of the biological characteristics of primary tumour tissue is critical for the study of early-stage solid tumour CAR-T therapies. Although some investigators have used this co-culture model to validate newly targeted CAR-T cells, optimise existing CAR-T cells and explore combination therapy strategies, there is still untapped potential in the co-culture models used today. This review introduces the current status of the application of tumour organoid and CAR-T cell co-culture models in recent years and commented on the limitations of the current co-cultivation model. Meanwhile, we compared the tumour organoid model with two pre-clinical models commonly used in CAR-T therapy research. Eventually, combined with the new progress of organoid technologies, optimization suggestions were proposed for the co-culture model from five perspectives: preserving or reconstructing the tumor microenvironment, systematization, vascularization, standardized culture procedures, and expanding the tumor organoids resource library, aimed at assisting related researchers to better utilize co-culture models. |
| first_indexed | 2024-04-25T01:02:20Z |
| format | Article |
| id | doaj.art-08eccc812de0434c964aa529148a4c9c |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-04-25T01:02:20Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-08eccc812de0434c964aa529148a4c9c2024-03-10T12:22:38ZengBMCCancer Cell International1475-28672024-03-0124111010.1186/s12935-024-03272-xApplication status and optimization suggestions of tumor organoids and CAR-T cell co-culture modelsRong-Xuan Ning0Cun-Yu Liu1Shi-Qi Wang2Wen-Kai Li3Xia Kong4Zhi-Wei He5The First Dongguan Affiliated Hospital, Guangdong Medical UniversityChina-America Cancer Research Institute, Guangdong Medical UniversityChina-America Cancer Research Institute, Guangdong Medical UniversityChina-America Cancer Research Institute, Guangdong Medical UniversityChina-America Cancer Research Institute, Guangdong Medical UniversityThe First Dongguan Affiliated Hospital, Guangdong Medical UniversityAbstract Tumor organoids, especially patient-derived organoids (PDOs) exhibit marked similarities in histopathological morphology, genomic alterations, and specific marker expression profiles to those of primary tumour tissues. They are applied in various fields including drug screening, gene editing, and identification of oncogenes. However, CAR-T therapy in the treatment of solid tumours is still at an exploratory stage. Tumour organoids offer unique advantages over other preclinical models commonly used for CAR-T therapy research, which the preservation of the biological characteristics of primary tumour tissue is critical for the study of early-stage solid tumour CAR-T therapies. Although some investigators have used this co-culture model to validate newly targeted CAR-T cells, optimise existing CAR-T cells and explore combination therapy strategies, there is still untapped potential in the co-culture models used today. This review introduces the current status of the application of tumour organoid and CAR-T cell co-culture models in recent years and commented on the limitations of the current co-cultivation model. Meanwhile, we compared the tumour organoid model with two pre-clinical models commonly used in CAR-T therapy research. Eventually, combined with the new progress of organoid technologies, optimization suggestions were proposed for the co-culture model from five perspectives: preserving or reconstructing the tumor microenvironment, systematization, vascularization, standardized culture procedures, and expanding the tumor organoids resource library, aimed at assisting related researchers to better utilize co-culture models.https://doi.org/10.1186/s12935-024-03272-xTumor organoidCAR-T therapyCo-culture models |
| spellingShingle | Rong-Xuan Ning Cun-Yu Liu Shi-Qi Wang Wen-Kai Li Xia Kong Zhi-Wei He Application status and optimization suggestions of tumor organoids and CAR-T cell co-culture models Cancer Cell International Tumor organoid CAR-T therapy Co-culture models |
| title | Application status and optimization suggestions of tumor organoids and CAR-T cell co-culture models |
| title_full | Application status and optimization suggestions of tumor organoids and CAR-T cell co-culture models |
| title_fullStr | Application status and optimization suggestions of tumor organoids and CAR-T cell co-culture models |
| title_full_unstemmed | Application status and optimization suggestions of tumor organoids and CAR-T cell co-culture models |
| title_short | Application status and optimization suggestions of tumor organoids and CAR-T cell co-culture models |
| title_sort | application status and optimization suggestions of tumor organoids and car t cell co culture models |
| topic | Tumor organoid CAR-T therapy Co-culture models |
| url | https://doi.org/10.1186/s12935-024-03272-x |
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