Detection of Arsenic(V) by Fluorescence Sensing Based on Chlorin e6-Copper Ion
The high toxicity of arsenic (As) can cause irreversible harm to the environment and human health. In this study, the chlorin e6 (Ce6), which emits fluorescence in the infrared region, was introduced as the luminescence center, and the addition of copper ion (Cu<sup>2+</sup>) and As(V) p...
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MDPI AG
2024-02-01
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| author | Mao-Ling Luo Guo-Ying Chen Jia-Li Wang Tong-Qing Chai Zheng-Ming Qian Wen-Jia Li Feng-Qing Yang |
| author_facet | Mao-Ling Luo Guo-Ying Chen Jia-Li Wang Tong-Qing Chai Zheng-Ming Qian Wen-Jia Li Feng-Qing Yang |
| author_sort | Mao-Ling Luo |
| collection | DOAJ |
| description | The high toxicity of arsenic (As) can cause irreversible harm to the environment and human health. In this study, the chlorin e6 (Ce6), which emits fluorescence in the infrared region, was introduced as the luminescence center, and the addition of copper ion (Cu<sup>2+</sup>) and As(V) provoked a regular change in fluorescence at 652 nm, whereas that of As(III) was 665 nm, which was used to optionally detect Cu<sup>2+</sup>, arsenic (As(III), and As(V)). The limit of detection (LOD) values were 0.212 μM, 0.089 ppm, and 1.375 ppb for Cu<sup>2+</sup>, As(III), and As(V), respectively. The developed method can be used to determine Cu<sup>2+</sup> and arsenic in water and soil with good sensitivity and selectivity. The 1:1 stoichiometry of Ce6 with Cu<sup>2+</sup> was obtained from the Job plot that was developed from UV–visible spectra. The binding constants for Cu<sup>2+</sup> and As(V) were established to be 1.248 × 10<sup>5</sup> M<sup>−1</sup> and 2.35 × 10<sup>12</sup> M<sup>−2</sup>, respectively, using B–H (Benesi–Hildebrand) plots. Fluorescence lifetimes, B–H plots, FT–IR, and <sup>1</sup>H-NMR were used to postulate the mechanism of Cu<sup>2+</sup> fluorescence quenching and As(V) fluorescence restoration and the interactions of the two ions with the Ce6 molecule. |
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| series | Molecules |
| spelling | doaj.art-08ef900cf56b41519bf96a0b4e32c05a2024-03-12T16:50:46ZengMDPI AGMolecules1420-30492024-02-01295101510.3390/molecules29051015Detection of Arsenic(V) by Fluorescence Sensing Based on Chlorin e6-Copper IonMao-Ling Luo0Guo-Ying Chen1Jia-Li Wang2Tong-Qing Chai3Zheng-Ming Qian4Wen-Jia Li5Feng-Qing Yang6School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, ChinaDongguan HEC Cordyceps R&D Co., Ltd., Dongguan 523850, ChinaDongguan HEC Cordyceps R&D Co., Ltd., Dongguan 523850, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, ChinaThe high toxicity of arsenic (As) can cause irreversible harm to the environment and human health. In this study, the chlorin e6 (Ce6), which emits fluorescence in the infrared region, was introduced as the luminescence center, and the addition of copper ion (Cu<sup>2+</sup>) and As(V) provoked a regular change in fluorescence at 652 nm, whereas that of As(III) was 665 nm, which was used to optionally detect Cu<sup>2+</sup>, arsenic (As(III), and As(V)). The limit of detection (LOD) values were 0.212 μM, 0.089 ppm, and 1.375 ppb for Cu<sup>2+</sup>, As(III), and As(V), respectively. The developed method can be used to determine Cu<sup>2+</sup> and arsenic in water and soil with good sensitivity and selectivity. The 1:1 stoichiometry of Ce6 with Cu<sup>2+</sup> was obtained from the Job plot that was developed from UV–visible spectra. The binding constants for Cu<sup>2+</sup> and As(V) were established to be 1.248 × 10<sup>5</sup> M<sup>−1</sup> and 2.35 × 10<sup>12</sup> M<sup>−2</sup>, respectively, using B–H (Benesi–Hildebrand) plots. Fluorescence lifetimes, B–H plots, FT–IR, and <sup>1</sup>H-NMR were used to postulate the mechanism of Cu<sup>2+</sup> fluorescence quenching and As(V) fluorescence restoration and the interactions of the two ions with the Ce6 molecule.https://www.mdpi.com/1420-3049/29/5/1015chlorin e6fluorescencecopperarsenic |
| spellingShingle | Mao-Ling Luo Guo-Ying Chen Jia-Li Wang Tong-Qing Chai Zheng-Ming Qian Wen-Jia Li Feng-Qing Yang Detection of Arsenic(V) by Fluorescence Sensing Based on Chlorin e6-Copper Ion Molecules chlorin e6 fluorescence copper arsenic |
| title | Detection of Arsenic(V) by Fluorescence Sensing Based on Chlorin e6-Copper Ion |
| title_full | Detection of Arsenic(V) by Fluorescence Sensing Based on Chlorin e6-Copper Ion |
| title_fullStr | Detection of Arsenic(V) by Fluorescence Sensing Based on Chlorin e6-Copper Ion |
| title_full_unstemmed | Detection of Arsenic(V) by Fluorescence Sensing Based on Chlorin e6-Copper Ion |
| title_short | Detection of Arsenic(V) by Fluorescence Sensing Based on Chlorin e6-Copper Ion |
| title_sort | detection of arsenic v by fluorescence sensing based on chlorin e6 copper ion |
| topic | chlorin e6 fluorescence copper arsenic |
| url | https://www.mdpi.com/1420-3049/29/5/1015 |
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