An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Variety ‘Aroma’
In strawberry micropropagation, several challenges must be overcome to obtain quality plants and achieve high survival rate to ex vitro acclimatization. In this study, therefore, a set of protocols were evaluated to optimize explant (meristem) disinfection, in vitro growth (multiplication and rootin...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/4/968 |
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| author | Juan C. Neri Jegnes Benjamín Meléndez-Mori José Jesús Tejada-Alvarado Nuri Carito Vilca-Valqui Eyner Huaman-Huaman Manuel Oliva Malluri Goñas |
| author_facet | Juan C. Neri Jegnes Benjamín Meléndez-Mori José Jesús Tejada-Alvarado Nuri Carito Vilca-Valqui Eyner Huaman-Huaman Manuel Oliva Malluri Goñas |
| author_sort | Juan C. Neri |
| collection | DOAJ |
| description | In strawberry micropropagation, several challenges must be overcome to obtain quality plants and achieve high survival rate to ex vitro acclimatization. In this study, therefore, a set of protocols were evaluated to optimize explant (meristem) disinfection, in vitro growth (multiplication and rooting), and ex vitro acclimatization of strawberry. The results showed that explants treated with 1.0% NaClO for 5 min had a lower percentage of contamination, and achieved a higher percentage of viability, height, and number of leaves. In vitro growth was favored by the use of 1 mg L<sup>−1</sup> zeatin, since it allowed greater seedling growth (number of shoots, seedling height, number of leaves, number of roots and root length), and a higher pre-acclimation rate (100%). In the acclimatization phase, plants grown in a substrate composed of compost + peat combined with 4 g of humic acid achieved better response in morphological and physiological variables. In fact, the results of this study could be used to cultivate strawberry plants of the ‘Aroma’ variety with high commercial quality. |
| first_indexed | 2024-03-09T11:15:32Z |
| format | Article |
| id | doaj.art-08f591e854944f769706292d87778e35 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T11:15:32Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-08f591e854944f769706292d87778e352023-12-01T00:29:36ZengMDPI AGAgronomy2073-43952022-04-0112496810.3390/agronomy12040968An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Variety ‘Aroma’Juan C. Neri0Jegnes Benjamín Meléndez-Mori1José Jesús Tejada-Alvarado2Nuri Carito Vilca-Valqui3Eyner Huaman-Huaman4Manuel Oliva5Malluri Goñas6Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, PeruInstituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, PeruInstituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, PeruInstituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, PeruInstituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, PeruInstituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, PeruInstituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, PeruIn strawberry micropropagation, several challenges must be overcome to obtain quality plants and achieve high survival rate to ex vitro acclimatization. In this study, therefore, a set of protocols were evaluated to optimize explant (meristem) disinfection, in vitro growth (multiplication and rooting), and ex vitro acclimatization of strawberry. The results showed that explants treated with 1.0% NaClO for 5 min had a lower percentage of contamination, and achieved a higher percentage of viability, height, and number of leaves. In vitro growth was favored by the use of 1 mg L<sup>−1</sup> zeatin, since it allowed greater seedling growth (number of shoots, seedling height, number of leaves, number of roots and root length), and a higher pre-acclimation rate (100%). In the acclimatization phase, plants grown in a substrate composed of compost + peat combined with 4 g of humic acid achieved better response in morphological and physiological variables. In fact, the results of this study could be used to cultivate strawberry plants of the ‘Aroma’ variety with high commercial quality.https://www.mdpi.com/2073-4395/12/4/968growth conditionsmeristem culturemorphophysiological characteristics |
| spellingShingle | Juan C. Neri Jegnes Benjamín Meléndez-Mori José Jesús Tejada-Alvarado Nuri Carito Vilca-Valqui Eyner Huaman-Huaman Manuel Oliva Malluri Goñas An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Variety ‘Aroma’ Agronomy growth conditions meristem culture morphophysiological characteristics |
| title | An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Variety ‘Aroma’ |
| title_full | An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Variety ‘Aroma’ |
| title_fullStr | An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Variety ‘Aroma’ |
| title_full_unstemmed | An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Variety ‘Aroma’ |
| title_short | An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Variety ‘Aroma’ |
| title_sort | optimized protocol for micropropagation and acclimatization of strawberry i fragaria i i ananassa i duch variety aroma |
| topic | growth conditions meristem culture morphophysiological characteristics |
| url | https://www.mdpi.com/2073-4395/12/4/968 |
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