Single-nucleotide polymorphisms in ialB, gltA and rpoB genes of Bartonella bacilliformis isolated from patients in endemic Peruvian regions.
Bartonella bacilliformis is a Gram-negative, aerobic bacterium and the known causal agent of Carrion's disease, still considered a neglected disease. There is limited information about the nucleotide sequences of this bacterium in international databases, and few studies have addressed the gene...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-10-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0011615 |
| _version_ | 1797659621291196416 |
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| author | Yanina Zarate-Sulca Karen Daphne Calvay-Sanchez Víctor Jimenez-Vasquez Joaquim Ruiz Oscar Acosta-Conchucos Giovanna Mendoza-Mujica |
| author_facet | Yanina Zarate-Sulca Karen Daphne Calvay-Sanchez Víctor Jimenez-Vasquez Joaquim Ruiz Oscar Acosta-Conchucos Giovanna Mendoza-Mujica |
| author_sort | Yanina Zarate-Sulca |
| collection | DOAJ |
| description | Bartonella bacilliformis is a Gram-negative, aerobic bacterium and the known causal agent of Carrion's disease, still considered a neglected disease. There is limited information about the nucleotide sequences of this bacterium in international databases, and few studies have addressed the genetic diversity of B. bacilliformis. We analyzed a total of 20 isolates of B. bacilliformis from the Peruvian regions of Ancash and Cajamarca. Three genes (ialB, gltA, and rpoB) were sequenced in each isolate and nucleotide sequences retrieved from GenBank (16 B. bacilliformis genomes) were also included in the study. All this information was merged in order to obtain clearer evidence of the phylogenetic relationships of B. bacilliformis. In the phylogenetic analysis conducted with the concatenated markers, four isolates (B.b-1, B. b-3, B. b- 7, B.b-8) from the Ancash region were observed to form a subgroup different from B. bacilliformis type strain KC583, showing dissimilarity levels of 5.96% (ialB), 3.69% (gltA) and 3.04% (rpoB). Our results suggest that B. bacilliformis consists of two different subgroups. Future investigations are needed to establish the taxonomic status of these subgroups. |
| first_indexed | 2024-03-11T18:16:49Z |
| format | Article |
| id | doaj.art-08f7ba01c89548edbcc17ef346d462d7 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-03-11T18:16:49Z |
| publishDate | 2023-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-08f7ba01c89548edbcc17ef346d462d72023-10-16T05:33:20ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352023-10-011710e001161510.1371/journal.pntd.0011615Single-nucleotide polymorphisms in ialB, gltA and rpoB genes of Bartonella bacilliformis isolated from patients in endemic Peruvian regions.Yanina Zarate-SulcaKaren Daphne Calvay-SanchezVíctor Jimenez-VasquezJoaquim RuizOscar Acosta-ConchucosGiovanna Mendoza-MujicaBartonella bacilliformis is a Gram-negative, aerobic bacterium and the known causal agent of Carrion's disease, still considered a neglected disease. There is limited information about the nucleotide sequences of this bacterium in international databases, and few studies have addressed the genetic diversity of B. bacilliformis. We analyzed a total of 20 isolates of B. bacilliformis from the Peruvian regions of Ancash and Cajamarca. Three genes (ialB, gltA, and rpoB) were sequenced in each isolate and nucleotide sequences retrieved from GenBank (16 B. bacilliformis genomes) were also included in the study. All this information was merged in order to obtain clearer evidence of the phylogenetic relationships of B. bacilliformis. In the phylogenetic analysis conducted with the concatenated markers, four isolates (B.b-1, B. b-3, B. b- 7, B.b-8) from the Ancash region were observed to form a subgroup different from B. bacilliformis type strain KC583, showing dissimilarity levels of 5.96% (ialB), 3.69% (gltA) and 3.04% (rpoB). Our results suggest that B. bacilliformis consists of two different subgroups. Future investigations are needed to establish the taxonomic status of these subgroups.https://doi.org/10.1371/journal.pntd.0011615 |
| spellingShingle | Yanina Zarate-Sulca Karen Daphne Calvay-Sanchez Víctor Jimenez-Vasquez Joaquim Ruiz Oscar Acosta-Conchucos Giovanna Mendoza-Mujica Single-nucleotide polymorphisms in ialB, gltA and rpoB genes of Bartonella bacilliformis isolated from patients in endemic Peruvian regions. PLoS Neglected Tropical Diseases |
| title | Single-nucleotide polymorphisms in ialB, gltA and rpoB genes of Bartonella bacilliformis isolated from patients in endemic Peruvian regions. |
| title_full | Single-nucleotide polymorphisms in ialB, gltA and rpoB genes of Bartonella bacilliformis isolated from patients in endemic Peruvian regions. |
| title_fullStr | Single-nucleotide polymorphisms in ialB, gltA and rpoB genes of Bartonella bacilliformis isolated from patients in endemic Peruvian regions. |
| title_full_unstemmed | Single-nucleotide polymorphisms in ialB, gltA and rpoB genes of Bartonella bacilliformis isolated from patients in endemic Peruvian regions. |
| title_short | Single-nucleotide polymorphisms in ialB, gltA and rpoB genes of Bartonella bacilliformis isolated from patients in endemic Peruvian regions. |
| title_sort | single nucleotide polymorphisms in ialb glta and rpob genes of bartonella bacilliformis isolated from patients in endemic peruvian regions |
| url | https://doi.org/10.1371/journal.pntd.0011615 |
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