| Summary: | To improve productivity, reduce production costs, and minimize the environmental impacts of agriculture, the advancement of nitrogen (N) fertilizer management methods is needed. The objective of this study is to compare the use of Unmanned Aerial Vehicle (UAV) multispectral imagery and PlanetScope satellite imagery, together with plant height, leaf area index (LAI), soil moisture, and field topographic metrics to predict the canopy nitrogen weight (g/m<sup>2</sup>) of wheat fields in southwestern Ontario, Canada. Random Forests (RF) and support vector regression (SVR) models, applied to either UAV imagery or satellite imagery, were evaluated for canopy nitrogen weight prediction. The top-performing UAV imagery-based validation model used SVR with seven selected variables (plant height, LAI, four VIs, and the NIR band) with an R<sup>2</sup> of 0.80 and an RMSE of 2.62 g/m<sup>2</sup>. The best satellite imagery-based validation model was RF, which used 17 variables including plant height, LAI, the four PlanetScope bands, and 11 VIs, resulting in an R<sup>2</sup> of 0.92 and an RMSE of 1.75 g/m<sup>2</sup>. The model information can be used to improve field nitrogen predictions for the effective management of N fertilizer.
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