Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test
A simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochlori...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2016-01-01
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| Series: | Open Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/chem-2016-0024 |
| _version_ | 1818727936958660608 |
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| author | Derayea Sayed M Omar Mahmoud A Abdel-Lateef Mohamed Aboel-Kasem Hassan Ahmed I. |
| author_facet | Derayea Sayed M Omar Mahmoud A Abdel-Lateef Mohamed Aboel-Kasem Hassan Ahmed I. |
| author_sort | Derayea Sayed M |
| collection | DOAJ |
| description | A simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochloride (PRO). The method is based on the quenching effect of the cited drugs on the fluorescence intensity of eosin Y at pH 3.4 (acetate buffer). The fluorescence quenching is due to the formation of an ion-pair complex and was measured without extraction at 545 nm (λex. 301.5 nm). The factors affecting the formation of the ion-pair complex were carefully studied and optimized. Under the optimal conditions, the linear ranges for the relationship between the fluorescence quenching value and the concentration of the investigated drugs were 100-2500, 150-2500 and 50-2250 ng mL-1 for (BTX, CAR), (LBT, NEB) and (PRO) respectively. The method was validated according to ICH guidelines and was applied for determination of the cited drugs in pharmaceutical dosage forms with excellent recoveries. In addition, content uniformity testing of some commercial dosage forms was checked by the proposed method. |
| first_indexed | 2024-12-17T22:22:01Z |
| format | Article |
| id | doaj.art-08f85458dc6b4d3a959117fa849da875 |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-12-17T22:22:01Z |
| publishDate | 2016-01-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-08f85458dc6b4d3a959117fa849da8752022-12-21T21:30:27ZengDe GruyterOpen Chemistry2391-54202016-01-0114125826610.1515/chem-2016-0024chem-2016-0024Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity testDerayea Sayed M0Omar Mahmoud A1Abdel-Lateef Mohamed Aboel-Kasem2Hassan Ahmed I.3Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut branch, Assiut 71524, EgyptDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut branch, Assiut 71524, EgyptA simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochloride (PRO). The method is based on the quenching effect of the cited drugs on the fluorescence intensity of eosin Y at pH 3.4 (acetate buffer). The fluorescence quenching is due to the formation of an ion-pair complex and was measured without extraction at 545 nm (λex. 301.5 nm). The factors affecting the formation of the ion-pair complex were carefully studied and optimized. Under the optimal conditions, the linear ranges for the relationship between the fluorescence quenching value and the concentration of the investigated drugs were 100-2500, 150-2500 and 50-2250 ng mL-1 for (BTX, CAR), (LBT, NEB) and (PRO) respectively. The method was validated according to ICH guidelines and was applied for determination of the cited drugs in pharmaceutical dosage forms with excellent recoveries. In addition, content uniformity testing of some commercial dosage forms was checked by the proposed method.https://doi.org/10.1515/chem-2016-0024β–adrenergic blockerseosin yion-pair complexationfluorescence quenchingpharmaceutical analysis |
| spellingShingle | Derayea Sayed M Omar Mahmoud A Abdel-Lateef Mohamed Aboel-Kasem Hassan Ahmed I. Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test Open Chemistry β–adrenergic blockers eosin y ion-pair complexation fluorescence quenching pharmaceutical analysis |
| title | Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test |
| title_full | Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test |
| title_fullStr | Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test |
| title_full_unstemmed | Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test |
| title_short | Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test |
| title_sort | development and validation of a new spectrofluorimetric method for the determination of some beta blockers through fluorescence quenching of eosin y application to content uniformity test |
| topic | β–adrenergic blockers eosin y ion-pair complexation fluorescence quenching pharmaceutical analysis |
| url | https://doi.org/10.1515/chem-2016-0024 |
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