Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control
Abstract Salmonella is a bacterium associated with food contaminated by various animals, primarily poultry. Interest and research on bacteriophages are increasing because they can be used as an alternative against increasing antibiotic resistance. In our study, eight Salmonella‐specific lytic bacter...
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| Format: | Article |
| Language: | English |
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Wiley
2024-04-01
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| Series: | Food Science & Nutrition |
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| Online Access: | https://doi.org/10.1002/fsn3.3956 |
| _version_ | 1797208898222948352 |
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| author | Aysegul Unverdi Hilal Basak Erol Banu Kaskatepe Orkun Babacan |
| author_facet | Aysegul Unverdi Hilal Basak Erol Banu Kaskatepe Orkun Babacan |
| author_sort | Aysegul Unverdi |
| collection | DOAJ |
| description | Abstract Salmonella is a bacterium associated with food contaminated by various animals, primarily poultry. Interest and research on bacteriophages are increasing because they can be used as an alternative against increasing antibiotic resistance. In our study, eight Salmonella‐specific lytic bacteriophages were isolated from chicken feces. Two of the isolated phages (AUFM_Sc1 and AUFM_Sc3) were chosen for their characterization due to their broader host range. Based on morphological and genomic analysis, AUFM_Sc1 was identified to be close to similar Enterobacteria spp. CC31 (Myoviridae) and AUFM_Sc3 was identified to be close to Salmonella phage vB_Sen_I1 (Demerecviridae (formerly Siphoviridae)). Although these phages have shown promise for use in phage therapy applications for chickens, further studies are needed on their suitability. When a cocktail of these phages (AUFM_Sc1 + AUFM_Sc3) and nisin combination was applied on chicken breast meat, it was determined that it was effective against Salmonella contamination and while a good inhibitory effect was observed on the food, especially during the first 48 h, the effect decreased later, but the bacterial concentration was still low compared to the control group. Therefore, it is considered that the combination of AUFM_Sc1 + AUFM_Sc3 + nisin can be used as a food preservative against Salmonella. |
| first_indexed | 2024-04-24T09:46:07Z |
| format | Article |
| id | doaj.art-08f862f80be64bb7b17a9650f4356d68 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-24T09:46:07Z |
| publishDate | 2024-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-08f862f80be64bb7b17a9650f4356d682024-04-15T01:06:44ZengWileyFood Science & Nutrition2048-71772024-04-011242760277110.1002/fsn3.3956Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐controlAysegul Unverdi0Hilal Basak Erol1Banu Kaskatepe2Orkun Babacan3Department of Pharmaceutical Microbiology Ankara University Faculty of Pharmacy Ankara TurkeyDepartment of Pharmaceutical Microbiology Ankara University Faculty of Pharmacy Ankara TurkeyDepartment of Pharmaceutical Microbiology Ankara University Faculty of Pharmacy Ankara TurkeyDepartment of Veterinary Science, Kepsut Vocational School Balıkesir University Kepsut, Balıkesir TurkeyAbstract Salmonella is a bacterium associated with food contaminated by various animals, primarily poultry. Interest and research on bacteriophages are increasing because they can be used as an alternative against increasing antibiotic resistance. In our study, eight Salmonella‐specific lytic bacteriophages were isolated from chicken feces. Two of the isolated phages (AUFM_Sc1 and AUFM_Sc3) were chosen for their characterization due to their broader host range. Based on morphological and genomic analysis, AUFM_Sc1 was identified to be close to similar Enterobacteria spp. CC31 (Myoviridae) and AUFM_Sc3 was identified to be close to Salmonella phage vB_Sen_I1 (Demerecviridae (formerly Siphoviridae)). Although these phages have shown promise for use in phage therapy applications for chickens, further studies are needed on their suitability. When a cocktail of these phages (AUFM_Sc1 + AUFM_Sc3) and nisin combination was applied on chicken breast meat, it was determined that it was effective against Salmonella contamination and while a good inhibitory effect was observed on the food, especially during the first 48 h, the effect decreased later, but the bacterial concentration was still low compared to the control group. Therefore, it is considered that the combination of AUFM_Sc1 + AUFM_Sc3 + nisin can be used as a food preservative against Salmonella.https://doi.org/10.1002/fsn3.3956food bio‐controlnisinphageSalmonella |
| spellingShingle | Aysegul Unverdi Hilal Basak Erol Banu Kaskatepe Orkun Babacan Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control Food Science & Nutrition food bio‐control nisin phage Salmonella |
| title | Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control |
| title_full | Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control |
| title_fullStr | Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control |
| title_full_unstemmed | Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control |
| title_short | Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control |
| title_sort | characterization of salmonella phages isolated from poultry coops and its effect with nisin on food bio control |
| topic | food bio‐control nisin phage Salmonella |
| url | https://doi.org/10.1002/fsn3.3956 |
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