A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive viruses
Abstract Background Chicken anemia virus (CAV), avian reovirus (ARV), infectious bursal disease virus (IBDV), Marek’s disease virus (MDV) and reticuloendotheliosis virus (REV) all cause immunosuppressive disease in birds through vertical or horizontal transmission. Mixed infections with these immuno...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-11-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1663-1 |
| _version_ | 1818999235894312960 |
|---|---|
| author | Feng Cong Yujun Zhu Jing Wang Yuexiao Lian Xiangnan Liu Li Xiao Ren Huang Yu Zhang Meili Chen Pengju Guo |
| author_facet | Feng Cong Yujun Zhu Jing Wang Yuexiao Lian Xiangnan Liu Li Xiao Ren Huang Yu Zhang Meili Chen Pengju Guo |
| author_sort | Feng Cong |
| collection | DOAJ |
| description | Abstract Background Chicken anemia virus (CAV), avian reovirus (ARV), infectious bursal disease virus (IBDV), Marek’s disease virus (MDV) and reticuloendotheliosis virus (REV) all cause immunosuppressive disease in birds through vertical or horizontal transmission. Mixed infections with these immunosuppressive pathogens lead to atypical clinical signs and obstruct accurate diagnoses and epidemiological investigations. Therefore, it is essential to develop a high-throughput assay for the simultaneous detection of these immunosuppressive viruses with high specificity and sensitivity. The aim of this study was to establish a novel method using a RT-PCR assay combined with fluorescence labeled polystyrene bead microarray (multiplex xTAG assay) to detect single or mixed viral infections. Results The results showed that the established xTAG assay had no nonspecific reactions with avian influenza virus (AIV), infectious bronchitis virus (IBV), newcastle disease virus (NDV), infectious laryngotracheitis virus (ILTV), Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). The limit of detection was 1.0 × 103 copies/μL for IBDV and 1.0 × 102copies/μL for the other four viruses. Ninety field samples were tested and the results were confirmed using conventional RT-PCR methods. The detection results of these two methods were 100% consistent. The established multiplex xTAG assay allows a high throughput and simultaneous detection of five chicken immunosuppressive viruses. Conclusion The multiplex xTAG assay has been showed to be an additional tool for molecular epidemiology studies of five chicken immunosuppressive viruses in the poultry industry. |
| first_indexed | 2024-12-20T22:14:12Z |
| format | Article |
| id | doaj.art-08f9c1623d1f4c2abbc2560414981c3a |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-20T22:14:12Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-08f9c1623d1f4c2abbc2560414981c3a2022-12-21T19:25:05ZengBMCBMC Veterinary Research1746-61482018-11-011411810.1186/s12917-018-1663-1A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive virusesFeng Cong0Yujun Zhu1Jing Wang2Yuexiao Lian3Xiangnan Liu4Li Xiao5Ren Huang6Yu Zhang7Meili Chen8Pengju Guo9Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory AnimalsGuangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory AnimalsGuangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory AnimalsGuangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory AnimalsGuangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory AnimalsGuangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory AnimalsGuangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory AnimalsGuangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory AnimalsGuangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory AnimalsGuangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory AnimalsAbstract Background Chicken anemia virus (CAV), avian reovirus (ARV), infectious bursal disease virus (IBDV), Marek’s disease virus (MDV) and reticuloendotheliosis virus (REV) all cause immunosuppressive disease in birds through vertical or horizontal transmission. Mixed infections with these immunosuppressive pathogens lead to atypical clinical signs and obstruct accurate diagnoses and epidemiological investigations. Therefore, it is essential to develop a high-throughput assay for the simultaneous detection of these immunosuppressive viruses with high specificity and sensitivity. The aim of this study was to establish a novel method using a RT-PCR assay combined with fluorescence labeled polystyrene bead microarray (multiplex xTAG assay) to detect single or mixed viral infections. Results The results showed that the established xTAG assay had no nonspecific reactions with avian influenza virus (AIV), infectious bronchitis virus (IBV), newcastle disease virus (NDV), infectious laryngotracheitis virus (ILTV), Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). The limit of detection was 1.0 × 103 copies/μL for IBDV and 1.0 × 102copies/μL for the other four viruses. Ninety field samples were tested and the results were confirmed using conventional RT-PCR methods. The detection results of these two methods were 100% consistent. The established multiplex xTAG assay allows a high throughput and simultaneous detection of five chicken immunosuppressive viruses. Conclusion The multiplex xTAG assay has been showed to be an additional tool for molecular epidemiology studies of five chicken immunosuppressive viruses in the poultry industry.http://link.springer.com/article/10.1186/s12917-018-1663-1Simultaneous detectionMultiplex xTAG assayChicken anemia virusAvian reovirusChicken immunosuppressive viruses |
| spellingShingle | Feng Cong Yujun Zhu Jing Wang Yuexiao Lian Xiangnan Liu Li Xiao Ren Huang Yu Zhang Meili Chen Pengju Guo A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive viruses BMC Veterinary Research Simultaneous detection Multiplex xTAG assay Chicken anemia virus Avian reovirus Chicken immunosuppressive viruses |
| title | A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive viruses |
| title_full | A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive viruses |
| title_fullStr | A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive viruses |
| title_full_unstemmed | A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive viruses |
| title_short | A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive viruses |
| title_sort | multiplex xtag assay for the simultaneous detection of five chicken immunosuppressive viruses |
| topic | Simultaneous detection Multiplex xTAG assay Chicken anemia virus Avian reovirus Chicken immunosuppressive viruses |
| url | http://link.springer.com/article/10.1186/s12917-018-1663-1 |
| work_keys_str_mv | AT fengcong amultiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT yujunzhu amultiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT jingwang amultiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT yuexiaolian amultiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT xiangnanliu amultiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT lixiao amultiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT renhuang amultiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT yuzhang amultiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT meilichen amultiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT pengjuguo amultiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT fengcong multiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT yujunzhu multiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT jingwang multiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT yuexiaolian multiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT xiangnanliu multiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT lixiao multiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT renhuang multiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT yuzhang multiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT meilichen multiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses AT pengjuguo multiplexxtagassayforthesimultaneousdetectionoffivechickenimmunosuppressiveviruses |