Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method

Introduction: The ABO blood group system was the significant element for forensic serological examination of blood and body fluids in the past before the wide adaptation of DNA typing. A significant proportion of individuals (80%) are secretors, meaning that antigens present in the blood are als...

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Main Authors: Rashmi Metgud, Nidhi Khajuria, Mamta, Gayathri Ramesh
Format: Article
Language:English
Published: JCDR Research and Publications Private Limited 2016-02-01
Series:Journal of Clinical and Diagnostic Research
Subjects:
Online Access:https://jcdr.net/articles/PDF/7161/11598_CE(RA1)_F(T)_PF1(Ek_Om)_PFA(AK)_PF2(PAG).pdf
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author Rashmi Metgud
Nidhi Khajuria
Mamta
Gayathri Ramesh
author_facet Rashmi Metgud
Nidhi Khajuria
Mamta
Gayathri Ramesh
author_sort Rashmi Metgud
collection DOAJ
description Introduction: The ABO blood group system was the significant element for forensic serological examination of blood and body fluids in the past before the wide adaptation of DNA typing. A significant proportion of individuals (80%) are secretors, meaning that antigens present in the blood are also found in other body fluids such as saliva. Absorption inhibition is one such method that works by reducing strength of an antiserum based on type and amount of antigen present in the stains. Aim: To check the efficacy of identifying the blood group antigens in saliva and to know the secretor status using absorption inhibition method among southern Rajasthan population. Materials and Methods: Blood and saliva samples were collected from 80 individuals comprising 20 individuals in each blood group. The absorption inhibition method was used to determine the blood group antigens in the saliva and then the results were correlated with the blood group of the collected blood sample. The compiled data was statistically analysed using chi-square test. Results: Blood groups A & O revealed 100% secretor status for both males and females. While blood groups B and AB revealed 95% secretor status. Conclusion: Secretor status evaluation of the ABO blood group antigen in saliva using absorption inhibition method can be a useful tool in forensic examination.
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spelling doaj.art-08fc20e941a74c49aae782e538e873292022-12-21T21:18:09ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-02-01102ZC01ZC0310.7860/JCDR/2016/11598.7161Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition MethodRashmi Metgud0Nidhi Khajuria1Mamta2Gayathri Ramesh3Professor and Head of Department, Department of Oral & Maxillofacial Pathology, Pacific Dental College, Udhaipur, India.Senior Lecturer, Department of Department of Oral & Maxillofacial Pathology, Institute of Dental Sceinces, Jammu, India.Post Graduate Student, Department of Oral & Maxillofacial Pathology, Pacific Dental College, Udhaipur, India.Reader, Department of Oral & Maxillofacial Pathology, Rama Dental College Hospital and Rescearch Center, Kanpur, Utter Predesh, India.Introduction: The ABO blood group system was the significant element for forensic serological examination of blood and body fluids in the past before the wide adaptation of DNA typing. A significant proportion of individuals (80%) are secretors, meaning that antigens present in the blood are also found in other body fluids such as saliva. Absorption inhibition is one such method that works by reducing strength of an antiserum based on type and amount of antigen present in the stains. Aim: To check the efficacy of identifying the blood group antigens in saliva and to know the secretor status using absorption inhibition method among southern Rajasthan population. Materials and Methods: Blood and saliva samples were collected from 80 individuals comprising 20 individuals in each blood group. The absorption inhibition method was used to determine the blood group antigens in the saliva and then the results were correlated with the blood group of the collected blood sample. The compiled data was statistically analysed using chi-square test. Results: Blood groups A & O revealed 100% secretor status for both males and females. While blood groups B and AB revealed 95% secretor status. Conclusion: Secretor status evaluation of the ABO blood group antigen in saliva using absorption inhibition method can be a useful tool in forensic examination.https://jcdr.net/articles/PDF/7161/11598_CE(RA1)_F(T)_PF1(Ek_Om)_PFA(AK)_PF2(PAG).pdfbody fluidblood stainforensic identificationhaemagglutinationsaliva analysis
spellingShingle Rashmi Metgud
Nidhi Khajuria
Mamta
Gayathri Ramesh
Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method
Journal of Clinical and Diagnostic Research
body fluid
blood stain
forensic identification
haemagglutination
saliva analysis
title Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method
title_full Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method
title_fullStr Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method
title_full_unstemmed Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method
title_short Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method
title_sort evaluation of the secretor status of abo blood group antigens in saliva among southern rajasthan population using absorption inhibition method
topic body fluid
blood stain
forensic identification
haemagglutination
saliva analysis
url https://jcdr.net/articles/PDF/7161/11598_CE(RA1)_F(T)_PF1(Ek_Om)_PFA(AK)_PF2(PAG).pdf
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AT nidhikhajuria evaluationofthesecretorstatusofabobloodgroupantigensinsalivaamongsouthernrajasthanpopulationusingabsorptioninhibitionmethod
AT mamta evaluationofthesecretorstatusofabobloodgroupantigensinsalivaamongsouthernrajasthanpopulationusingabsorptioninhibitionmethod
AT gayathriramesh evaluationofthesecretorstatusofabobloodgroupantigensinsalivaamongsouthernrajasthanpopulationusingabsorptioninhibitionmethod