Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method
Introduction: The ABO blood group system was the significant element for forensic serological examination of blood and body fluids in the past before the wide adaptation of DNA typing. A significant proportion of individuals (80%) are secretors, meaning that antigens present in the blood are als...
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Format: | Article |
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JCDR Research and Publications Private Limited
2016-02-01
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Series: | Journal of Clinical and Diagnostic Research |
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Online Access: | https://jcdr.net/articles/PDF/7161/11598_CE(RA1)_F(T)_PF1(Ek_Om)_PFA(AK)_PF2(PAG).pdf |
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author | Rashmi Metgud Nidhi Khajuria Mamta Gayathri Ramesh |
author_facet | Rashmi Metgud Nidhi Khajuria Mamta Gayathri Ramesh |
author_sort | Rashmi Metgud |
collection | DOAJ |
description | Introduction: The ABO blood group system was the significant
element for forensic serological examination of blood and body
fluids in the past before the wide adaptation of DNA typing. A
significant proportion of individuals (80%) are secretors, meaning
that antigens present in the blood are also found in other body
fluids such as saliva. Absorption inhibition is one such method that
works by reducing strength of an antiserum based on type and
amount of antigen present in the stains.
Aim: To check the efficacy of identifying the blood group antigens
in saliva and to know the secretor status using absorption inhibition
method among southern Rajasthan population.
Materials and Methods: Blood and saliva samples were collected
from 80 individuals comprising 20 individuals in each blood group.
The absorption inhibition method was used to determine the blood
group antigens in the saliva and then the results were correlated
with the blood group of the collected blood sample. The compiled
data was statistically analysed using chi-square test.
Results: Blood groups A & O revealed 100% secretor status for
both males and females. While blood groups B and AB revealed
95% secretor status.
Conclusion: Secretor status evaluation of the ABO blood group
antigen in saliva using absorption inhibition method can be a useful
tool in forensic examination. |
first_indexed | 2024-12-18T06:20:49Z |
format | Article |
id | doaj.art-08fc20e941a74c49aae782e538e87329 |
institution | Directory Open Access Journal |
issn | 2249-782X 0973-709X |
language | English |
last_indexed | 2024-12-18T06:20:49Z |
publishDate | 2016-02-01 |
publisher | JCDR Research and Publications Private Limited |
record_format | Article |
series | Journal of Clinical and Diagnostic Research |
spelling | doaj.art-08fc20e941a74c49aae782e538e873292022-12-21T21:18:09ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-02-01102ZC01ZC0310.7860/JCDR/2016/11598.7161Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition MethodRashmi Metgud0Nidhi Khajuria1Mamta2Gayathri Ramesh3Professor and Head of Department, Department of Oral & Maxillofacial Pathology, Pacific Dental College, Udhaipur, India.Senior Lecturer, Department of Department of Oral & Maxillofacial Pathology, Institute of Dental Sceinces, Jammu, India.Post Graduate Student, Department of Oral & Maxillofacial Pathology, Pacific Dental College, Udhaipur, India.Reader, Department of Oral & Maxillofacial Pathology, Rama Dental College Hospital and Rescearch Center, Kanpur, Utter Predesh, India.Introduction: The ABO blood group system was the significant element for forensic serological examination of blood and body fluids in the past before the wide adaptation of DNA typing. A significant proportion of individuals (80%) are secretors, meaning that antigens present in the blood are also found in other body fluids such as saliva. Absorption inhibition is one such method that works by reducing strength of an antiserum based on type and amount of antigen present in the stains. Aim: To check the efficacy of identifying the blood group antigens in saliva and to know the secretor status using absorption inhibition method among southern Rajasthan population. Materials and Methods: Blood and saliva samples were collected from 80 individuals comprising 20 individuals in each blood group. The absorption inhibition method was used to determine the blood group antigens in the saliva and then the results were correlated with the blood group of the collected blood sample. The compiled data was statistically analysed using chi-square test. Results: Blood groups A & O revealed 100% secretor status for both males and females. While blood groups B and AB revealed 95% secretor status. Conclusion: Secretor status evaluation of the ABO blood group antigen in saliva using absorption inhibition method can be a useful tool in forensic examination.https://jcdr.net/articles/PDF/7161/11598_CE(RA1)_F(T)_PF1(Ek_Om)_PFA(AK)_PF2(PAG).pdfbody fluidblood stainforensic identificationhaemagglutinationsaliva analysis |
spellingShingle | Rashmi Metgud Nidhi Khajuria Mamta Gayathri Ramesh Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method Journal of Clinical and Diagnostic Research body fluid blood stain forensic identification haemagglutination saliva analysis |
title | Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method |
title_full | Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method |
title_fullStr | Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method |
title_full_unstemmed | Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method |
title_short | Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method |
title_sort | evaluation of the secretor status of abo blood group antigens in saliva among southern rajasthan population using absorption inhibition method |
topic | body fluid blood stain forensic identification haemagglutination saliva analysis |
url | https://jcdr.net/articles/PDF/7161/11598_CE(RA1)_F(T)_PF1(Ek_Om)_PFA(AK)_PF2(PAG).pdf |
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