| Summary: | Numerous <i>Salmonella</i> <i>enterica</i> serovars can cause disease and contamination of animal-produced foods. Oligosaccharide-rich products capable of blocking pathogen adherence to intestinal mucosa are attractive alternatives to antibiotics as these have potential to prevent enteric infections. Presently, a wood-derived prebiotic composed mainly of glucose-galactose-mannose-xylose oligomers was found to inhibit mannose-sensitive binding of select <i>Salmonella</i> <i>Typhimurium</i> and <i>Escherichia coli</i> strains when reacted with <i>Saccharomyces boulardii</i>. Tests for the ability of the prebiotic to prevent binding of a green fluorescent protein (GFP)-labeled <i>S.</i> <i>Typhimurium</i> to intestinal porcine epithelial cells (IPEC-J2) cultured in vitro revealed that prebiotic-exposed GFP-labeled <i>S.</i> <i>Typhimurium</i> bound > 30% fewer individual IPEC-J2 cells than did GFP-labeled <i>S.</i> <i>Typhimurium</i> having no prebiotic exposure. Quantitatively, 90% fewer prebiotic-exposed GFP-labeled <i>S.</i> <i>Typhimurium</i> cells were bound per individual IPEC-J2 cell compared to non-prebiotic exposed GFP-labeled <i>S.</i> <i>Typhimurium</i>. Comparison of invasiveness of <i>S.</i> <i>Typhimurium</i> DT104 against IPEC-J2 cells revealed greater than a 90% decrease in intracellular recovery of prebiotic-exposed <i>S.</i> <i>Typhimurium</i> DT104 compared to non-exposed controls (averaging 4.4 ± 0.2 log<sub>10</sub> CFU/well). These results suggest compounds within the wood-derived prebiotic bound to <i>E. coli</i> and <i>S.</i> <i>Typhimurium</i>-produced adhesions and in the case of <i>S.</i> <i>Typhimurium</i>, this adhesion-binding activity inhibited the binding and invasion of IPEC-J2 cells.
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