Expression of adenosine triphosphate-sensitive potassium channels in rats with cirrhosis: correlationship with sympathetic activity and renal function
Objective: The aim of this study was to perform a direct analysis ofKATP mRNA expression by RT-PCR in kidney and isolated aorta fromrats with cirrhosis (induced by carbon tetrachloride) and controls.The present study also analyses the relation between induced cirrhosisand urinary excretion of sodium...
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| Format: | Article |
| Language: | English |
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
2006-12-01
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| Series: | Einstein (São Paulo) |
| Subjects: | |
| Online Access: | http://www.einstein.br/revista/arquivos/PDF/445-Einstein_vol4_n4_2006_p293.pdf |
| _version_ | 1818381909501149184 |
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| author | Julio Cesar Martins Monte Marcelino de Souza Durão Júnior Marcelo Costa Batista Miriam Boim Dulce Casarini Miguel Cendoroglo Neto Oscar Fernando Pavão dos Santos |
| author_facet | Julio Cesar Martins Monte Marcelino de Souza Durão Júnior Marcelo Costa Batista Miriam Boim Dulce Casarini Miguel Cendoroglo Neto Oscar Fernando Pavão dos Santos |
| author_sort | Julio Cesar Martins Monte |
| collection | DOAJ |
| description | Objective: The aim of this study was to perform a direct analysis ofKATP mRNA expression by RT-PCR in kidney and isolated aorta fromrats with cirrhosis (induced by carbon tetrachloride) and controls.The present study also analyses the relation between induced cirrhosisand urinary excretion of sodium and sympathetic activity in cirrhoticrats. Methods: Rats were placed in metabolic cages and allowedfree access to food and water. Cirrhosis was induced by repeateddoses of carbon tetrachloride by gastric gavage. After some weeks,the kidney and aorta were dissected and utilized for RNA extraction.Blood and urine were analyzed for electrolytes. Renal function wasestimated by creatinine clearance and sodium urinary excretion.Serum catecholamines were measured by HPLC analysis. Results:First, RT-PCR analysis showed that KATP mRNA is expressed in liverwith cirrhosis and intense fibrosis, but not with moderate fibrosis.Second, RT-PCR analysis revealed that KATP mRNA was detectedonly in aorta dissected from rats with cirrhosis. Finally, an enhancedreabsorption of sodium without renal failure suggests a potentialmediator would increase the activity of the sympathetic system.Conclusion: These results suggest that KATP mRNA is expressed incirrhotic rats with sympathetic activation and renal dysfunction. Thischannel might be involved in another route where the vascular tonecan be modulated in cirrhosis. |
| first_indexed | 2024-12-14T02:42:04Z |
| format | Article |
| id | doaj.art-090117fb875044b8acf5e6f629ecae00 |
| institution | Directory Open Access Journal |
| issn | 1679-4508 |
| language | English |
| last_indexed | 2024-12-14T02:42:04Z |
| publishDate | 2006-12-01 |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-090117fb875044b8acf5e6f629ecae002022-12-21T23:19:59ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)1679-45082006-12-0144293297Expression of adenosine triphosphate-sensitive potassium channels in rats with cirrhosis: correlationship with sympathetic activity and renal functionJulio Cesar Martins MonteMarcelino de Souza Durão JúniorMarcelo Costa BatistaMiriam BoimDulce CasariniMiguel Cendoroglo NetoOscar Fernando Pavão dos SantosObjective: The aim of this study was to perform a direct analysis ofKATP mRNA expression by RT-PCR in kidney and isolated aorta fromrats with cirrhosis (induced by carbon tetrachloride) and controls.The present study also analyses the relation between induced cirrhosisand urinary excretion of sodium and sympathetic activity in cirrhoticrats. Methods: Rats were placed in metabolic cages and allowedfree access to food and water. Cirrhosis was induced by repeateddoses of carbon tetrachloride by gastric gavage. After some weeks,the kidney and aorta were dissected and utilized for RNA extraction.Blood and urine were analyzed for electrolytes. Renal function wasestimated by creatinine clearance and sodium urinary excretion.Serum catecholamines were measured by HPLC analysis. Results:First, RT-PCR analysis showed that KATP mRNA is expressed in liverwith cirrhosis and intense fibrosis, but not with moderate fibrosis.Second, RT-PCR analysis revealed that KATP mRNA was detectedonly in aorta dissected from rats with cirrhosis. Finally, an enhancedreabsorption of sodium without renal failure suggests a potentialmediator would increase the activity of the sympathetic system.Conclusion: These results suggest that KATP mRNA is expressed incirrhotic rats with sympathetic activation and renal dysfunction. Thischannel might be involved in another route where the vascular tonecan be modulated in cirrhosis.http://www.einstein.br/revista/arquivos/PDF/445-Einstein_vol4_n4_2006_p293.pdfPotassium channels/analysisAdenosine triphosphateSympathetic nervous system/pathophysiologyLiver cirrhosisexperimentalKidney/physiologySodium/urineCarbon tetrachlorideRats |
| spellingShingle | Julio Cesar Martins Monte Marcelino de Souza Durão Júnior Marcelo Costa Batista Miriam Boim Dulce Casarini Miguel Cendoroglo Neto Oscar Fernando Pavão dos Santos Expression of adenosine triphosphate-sensitive potassium channels in rats with cirrhosis: correlationship with sympathetic activity and renal function Einstein (São Paulo) Potassium channels/analysis Adenosine triphosphate Sympathetic nervous system/pathophysiology Liver cirrhosis experimental Kidney/physiology Sodium/urine Carbon tetrachloride Rats |
| title | Expression of adenosine triphosphate-sensitive potassium channels in rats with cirrhosis: correlationship with sympathetic activity and renal function |
| title_full | Expression of adenosine triphosphate-sensitive potassium channels in rats with cirrhosis: correlationship with sympathetic activity and renal function |
| title_fullStr | Expression of adenosine triphosphate-sensitive potassium channels in rats with cirrhosis: correlationship with sympathetic activity and renal function |
| title_full_unstemmed | Expression of adenosine triphosphate-sensitive potassium channels in rats with cirrhosis: correlationship with sympathetic activity and renal function |
| title_short | Expression of adenosine triphosphate-sensitive potassium channels in rats with cirrhosis: correlationship with sympathetic activity and renal function |
| title_sort | expression of adenosine triphosphate sensitive potassium channels in rats with cirrhosis correlationship with sympathetic activity and renal function |
| topic | Potassium channels/analysis Adenosine triphosphate Sympathetic nervous system/pathophysiology Liver cirrhosis experimental Kidney/physiology Sodium/urine Carbon tetrachloride Rats |
| url | http://www.einstein.br/revista/arquivos/PDF/445-Einstein_vol4_n4_2006_p293.pdf |
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