Evidence for Chlamydiaceae and Parachlamydiaceae in a wild boar (Sus scrofa) population in Italy
Conjunctival swabs from 44 free-living wild boars culled during a demographic control programme applied in a Regional Park located in the Northern Italy were examined by 16S rRNA encoding gene nested PCR. In total, 22 (50%) wild boars were PCR positive. Sequencing of the amplicons identified Chlamyd...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2013-03-01
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| Series: | Veterinaria Italiana |
| Subjects: | |
| Online Access: | http://www.izs.it/vet_italiana/2013/49_1/119.pdf |
| _version_ | 1818957242401030144 |
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| author | Antonietta Di Francesco Raffaella Baldelli Manuela Donati Claudia Cotti Patrizia Bassi Mauro Delogu |
| author_facet | Antonietta Di Francesco Raffaella Baldelli Manuela Donati Claudia Cotti Patrizia Bassi Mauro Delogu |
| author_sort | Antonietta Di Francesco |
| collection | DOAJ |
| description | Conjunctival swabs from 44 free-living wild boars culled during a demographic control programme applied in a Regional Park located in the Northern Italy were examined by 16S rRNA encoding gene nested PCR. In total, 22 (50%) wild boars were PCR positive. Sequencing of the amplicons identified Chlamydia suis and Chlamydia pecorum in 12 and 5 samples, respectively. For one sample found PCR positive, the nucleotide sequence could not be determined. Four conjunctival samples showed ≥ 92% sequence similarities to 16S rRNA sequences from Chlamydia-like organisms, as did large intestine, uterus, and vaginal swabs from the same four animals. Amoeba DNA was found in one Chlamydia-like organism positive conjunctival swab. To our knowledge, this is the first detection of members of the Parachlamydiaceae family in wild boars, confirming a large animal host range for Chlamydia-like organisms. |
| first_indexed | 2024-12-20T11:06:44Z |
| format | Article |
| id | doaj.art-0901efbddb08442fb2c8c0796dd27ca6 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-12-20T11:06:44Z |
| publishDate | 2013-03-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-0901efbddb08442fb2c8c0796dd27ca62022-12-21T19:42:50ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272013-03-01491119122Evidence for Chlamydiaceae and Parachlamydiaceae in a wild boar (Sus scrofa) population in ItalyAntonietta Di FrancescoRaffaella BaldelliManuela DonatiClaudia CottiPatrizia BassiMauro DeloguConjunctival swabs from 44 free-living wild boars culled during a demographic control programme applied in a Regional Park located in the Northern Italy were examined by 16S rRNA encoding gene nested PCR. In total, 22 (50%) wild boars were PCR positive. Sequencing of the amplicons identified Chlamydia suis and Chlamydia pecorum in 12 and 5 samples, respectively. For one sample found PCR positive, the nucleotide sequence could not be determined. Four conjunctival samples showed ≥ 92% sequence similarities to 16S rRNA sequences from Chlamydia-like organisms, as did large intestine, uterus, and vaginal swabs from the same four animals. Amoeba DNA was found in one Chlamydia-like organism positive conjunctival swab. To our knowledge, this is the first detection of members of the Parachlamydiaceae family in wild boars, confirming a large animal host range for Chlamydia-like organisms.http://www.izs.it/vet_italiana/2013/49_1/119.pdfChlamydiaceaeItalyNested PCRParachlamydiaceaeWild boar |
| spellingShingle | Antonietta Di Francesco Raffaella Baldelli Manuela Donati Claudia Cotti Patrizia Bassi Mauro Delogu Evidence for Chlamydiaceae and Parachlamydiaceae in a wild boar (Sus scrofa) population in Italy Veterinaria Italiana Chlamydiaceae Italy Nested PCR Parachlamydiaceae Wild boar |
| title | Evidence for Chlamydiaceae and Parachlamydiaceae in a wild boar (Sus scrofa) population in Italy |
| title_full | Evidence for Chlamydiaceae and Parachlamydiaceae in a wild boar (Sus scrofa) population in Italy |
| title_fullStr | Evidence for Chlamydiaceae and Parachlamydiaceae in a wild boar (Sus scrofa) population in Italy |
| title_full_unstemmed | Evidence for Chlamydiaceae and Parachlamydiaceae in a wild boar (Sus scrofa) population in Italy |
| title_short | Evidence for Chlamydiaceae and Parachlamydiaceae in a wild boar (Sus scrofa) population in Italy |
| title_sort | evidence for chlamydiaceae and parachlamydiaceae in a wild boar sus scrofa population in italy |
| topic | Chlamydiaceae Italy Nested PCR Parachlamydiaceae Wild boar |
| url | http://www.izs.it/vet_italiana/2013/49_1/119.pdf |
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