In vitro effects of citral on Trypanosoma cruzi metacyclogenesis
Citral, the main constituent of lemongrass (Cymbopogon citratus) essential oil, was added to Trypanosoma cruzi cultures grown in TAU3AAG medium to observe the effect on the epimastigote-to-trypomastigote differentiation process (metacyclogenesis). Our results showed that citral (20 μg/mL) did not af...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2010-12-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000800012&lng=en&tlng=en |
| _version_ | 1797718493915774976 |
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| author | Josiane Cardoso Maurilio José Soares |
| author_facet | Josiane Cardoso Maurilio José Soares |
| author_sort | Josiane Cardoso |
| collection | DOAJ |
| description | Citral, the main constituent of lemongrass (Cymbopogon citratus) essential oil, was added to Trypanosoma cruzi cultures grown in TAU3AAG medium to observe the effect on the epimastigote-to-trypomastigote differentiation process (metacyclogenesis). Our results showed that citral (20 μg/mL) did not affect epimastigote viability or inhibit the differentiation process. Concentrations higher than 60 μg/mL, however, led to 100% cell death (both epimastigote and trypomastigote forms). Although epimastigotes incubated with 30 μg/mL citral were viable and able to adhere to the substrate, we observed around 50% inhibition in metacyclogenesis, with a calculated concentration that inhibited metacyclogenesis by 50% after 24 h (IC50/24 h) of about 31 μg/mL. Treatment with 30 μg/mL citral did not hinder epimastigote multiplication because epimastigote growth resumed when treated cells were transferred to a drug-free liver infusion tryptose culture medium. Metacyclogenesis was almost totally abolished at 40 μg/mL after 24 h of incubation. Furthermore, the metacyclic trypomastigotes obtained in vitro were similarly susceptible to citral, with an IC50/24 h, concentration that killed 50% of the cells after 24 h, of about 24.5 μg/mL. Therefore, citral appears to be a good candidate as an inhibitory drug for further studies analyzing the T. cruzi metacyclogenesis process. |
| first_indexed | 2024-03-12T08:51:32Z |
| format | Article |
| id | doaj.art-09032ea032ae436db8cd988bf8f83eff |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:51:32Z |
| publishDate | 2010-12-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-09032ea032ae436db8cd988bf8f83eff2023-09-02T16:17:49ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602010-12-0110581026103210.1590/S0074-02762010000800012S0074-02762010000800012In vitro effects of citral on Trypanosoma cruzi metacyclogenesisJosiane Cardoso0Maurilio José Soares1Fundação Oswaldo CruzFundação Oswaldo CruzCitral, the main constituent of lemongrass (Cymbopogon citratus) essential oil, was added to Trypanosoma cruzi cultures grown in TAU3AAG medium to observe the effect on the epimastigote-to-trypomastigote differentiation process (metacyclogenesis). Our results showed that citral (20 μg/mL) did not affect epimastigote viability or inhibit the differentiation process. Concentrations higher than 60 μg/mL, however, led to 100% cell death (both epimastigote and trypomastigote forms). Although epimastigotes incubated with 30 μg/mL citral were viable and able to adhere to the substrate, we observed around 50% inhibition in metacyclogenesis, with a calculated concentration that inhibited metacyclogenesis by 50% after 24 h (IC50/24 h) of about 31 μg/mL. Treatment with 30 μg/mL citral did not hinder epimastigote multiplication because epimastigote growth resumed when treated cells were transferred to a drug-free liver infusion tryptose culture medium. Metacyclogenesis was almost totally abolished at 40 μg/mL after 24 h of incubation. Furthermore, the metacyclic trypomastigotes obtained in vitro were similarly susceptible to citral, with an IC50/24 h, concentration that killed 50% of the cells after 24 h, of about 24.5 μg/mL. Therefore, citral appears to be a good candidate as an inhibitory drug for further studies analyzing the T. cruzi metacyclogenesis process.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000800012&lng=en&tlng=encitralessential oilmetacyclogenesisTrypanosoma cruzi |
| spellingShingle | Josiane Cardoso Maurilio José Soares In vitro effects of citral on Trypanosoma cruzi metacyclogenesis Memorias do Instituto Oswaldo Cruz citral essential oil metacyclogenesis Trypanosoma cruzi |
| title | In vitro effects of citral on Trypanosoma cruzi metacyclogenesis |
| title_full | In vitro effects of citral on Trypanosoma cruzi metacyclogenesis |
| title_fullStr | In vitro effects of citral on Trypanosoma cruzi metacyclogenesis |
| title_full_unstemmed | In vitro effects of citral on Trypanosoma cruzi metacyclogenesis |
| title_short | In vitro effects of citral on Trypanosoma cruzi metacyclogenesis |
| title_sort | in vitro effects of citral on trypanosoma cruzi metacyclogenesis |
| topic | citral essential oil metacyclogenesis Trypanosoma cruzi |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000800012&lng=en&tlng=en |
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