Polimeric Immunoglobulin Diagnosticum for Detection of Cholera Toxin and Assessing the Level of Its Production by Vibrios
A marker of the epidemic significance of Vibrio cholerae is their toxigenicity. Therefore, much attention is currently paid to the creation of diagnostic preparations for the detection of cholera toxin and assessment of the level of its production. The volumetric immunosuspension agglomeration react...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2022-01-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/1595 |
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| author | L. V. Larionova R. V. Pisanov D. I. Simakova A. N. Narkevich I. V. Arkhangel’skaya |
| author_facet | L. V. Larionova R. V. Pisanov D. I. Simakova A. N. Narkevich I. V. Arkhangel’skaya |
| author_sort | L. V. Larionova |
| collection | DOAJ |
| description | A marker of the epidemic significance of Vibrio cholerae is their toxigenicity. Therefore, much attention is currently paid to the creation of diagnostic preparations for the detection of cholera toxin and assessment of the level of its production. The volumetric immunosuspension agglomeration reaction, carried out with the help of latex diagnosticums, is an analogue of the indirect hemagglutination reaction, an affordable and technically simple method, since it does not require special equipment and can be used when conducting research in the field. The aim of the study was to design a polymeric immunoglobulin diagnosticum for determining cholera toxin and the level of its production by vibrio strains. Materials and methods. Cholera toxin was obtained from the producer strain Vibrio cholerae Classical 569 B. Rabbit serum to the toxin was obtained according to the method selected by the authors. A polymeric diagnostic immunoglobulin antitoxic drug was obtained through sensitizing immunoglobulins from cholera antitoxic rabbit serum on the surface of polyacrolein microspheres with a size of (1±0.1) μm. Results and discussion. The analytical sensitivity of the developed diagnostic preparation with control cholera toxin is 100 ng/ml. It detects cholera toxin in toxigenic strains of Vibrio cholerae in a titer of 1:16 – 1:512, gives negative results with non-toxigenic strains of V. cholerae O1, V. Cholerae nonO1/nonO139, with samples of heterologous cultures, LPS preparations, liquid nutrient medium used for the cultivation of V. cholerae. Thus, a polymeric immunoglobulin diagnosticum has been constructed to detect and quantify the production of cholera toxin by vibrio strains, and its analytical sensitivity and specificity have been established. |
| first_indexed | 2024-03-08T13:40:53Z |
| format | Article |
| id | doaj.art-0903ac9997434e29b8120247181f65c5 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2025-02-17T14:07:40Z |
| publishDate | 2022-01-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-0903ac9997434e29b8120247181f65c52024-12-24T12:09:56ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2022-01-0104848910.21055/0370-1069-2021-4-84-891334Polimeric Immunoglobulin Diagnosticum for Detection of Cholera Toxin and Assessing the Level of Its Production by VibriosL. V. Larionova0R. V. Pisanov1D. I. Simakova2A. N. Narkevich3I. V. Arkhangel’skaya4Rostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteA marker of the epidemic significance of Vibrio cholerae is their toxigenicity. Therefore, much attention is currently paid to the creation of diagnostic preparations for the detection of cholera toxin and assessment of the level of its production. The volumetric immunosuspension agglomeration reaction, carried out with the help of latex diagnosticums, is an analogue of the indirect hemagglutination reaction, an affordable and technically simple method, since it does not require special equipment and can be used when conducting research in the field. The aim of the study was to design a polymeric immunoglobulin diagnosticum for determining cholera toxin and the level of its production by vibrio strains. Materials and methods. Cholera toxin was obtained from the producer strain Vibrio cholerae Classical 569 B. Rabbit serum to the toxin was obtained according to the method selected by the authors. A polymeric diagnostic immunoglobulin antitoxic drug was obtained through sensitizing immunoglobulins from cholera antitoxic rabbit serum on the surface of polyacrolein microspheres with a size of (1±0.1) μm. Results and discussion. The analytical sensitivity of the developed diagnostic preparation with control cholera toxin is 100 ng/ml. It detects cholera toxin in toxigenic strains of Vibrio cholerae in a titer of 1:16 – 1:512, gives negative results with non-toxigenic strains of V. cholerae O1, V. Cholerae nonO1/nonO139, with samples of heterologous cultures, LPS preparations, liquid nutrient medium used for the cultivation of V. cholerae. Thus, a polymeric immunoglobulin diagnosticum has been constructed to detect and quantify the production of cholera toxin by vibrio strains, and its analytical sensitivity and specificity have been established.https://journal.microbe.ru/jour/article/view/1595vibrio choleraecholera toxinimmunoglobulinpolymeric microspheresdiagnosticumanalytical sensitivityanalytical specificity |
| spellingShingle | L. V. Larionova R. V. Pisanov D. I. Simakova A. N. Narkevich I. V. Arkhangel’skaya Polimeric Immunoglobulin Diagnosticum for Detection of Cholera Toxin and Assessing the Level of Its Production by Vibrios Проблемы особо опасных инфекций vibrio cholerae cholera toxin immunoglobulin polymeric microspheres diagnosticum analytical sensitivity analytical specificity |
| title | Polimeric Immunoglobulin Diagnosticum for Detection of Cholera Toxin and Assessing the Level of Its Production by Vibrios |
| title_full | Polimeric Immunoglobulin Diagnosticum for Detection of Cholera Toxin and Assessing the Level of Its Production by Vibrios |
| title_fullStr | Polimeric Immunoglobulin Diagnosticum for Detection of Cholera Toxin and Assessing the Level of Its Production by Vibrios |
| title_full_unstemmed | Polimeric Immunoglobulin Diagnosticum for Detection of Cholera Toxin and Assessing the Level of Its Production by Vibrios |
| title_short | Polimeric Immunoglobulin Diagnosticum for Detection of Cholera Toxin and Assessing the Level of Its Production by Vibrios |
| title_sort | polimeric immunoglobulin diagnosticum for detection of cholera toxin and assessing the level of its production by vibrios |
| topic | vibrio cholerae cholera toxin immunoglobulin polymeric microspheres diagnosticum analytical sensitivity analytical specificity |
| url | https://journal.microbe.ru/jour/article/view/1595 |
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