Comparative Investigation of Raw and Processed Radix Polygoni Multiflori on the Treatment of Vascular Dementia by Liquid Chromatograph−Mass Spectrometry Based Metabolomic Approach
Radix Polygoni Multiflori (PM) is a well−known nootropic used in traditional Chinese medicine (TCM). Considering the efficacy and application discrepancy between raw (RPM) and processed PM (PPM), the similarities and differences between them in the treatment of vascular dementia (VaD) is intriguing....
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/12/1297 |
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| author | Fengye Wu Yunlin Li Wenya Liu Ran Xiao Benxing Yao Mingzhe Gao Di Xu Junsong Wang |
| author_facet | Fengye Wu Yunlin Li Wenya Liu Ran Xiao Benxing Yao Mingzhe Gao Di Xu Junsong Wang |
| author_sort | Fengye Wu |
| collection | DOAJ |
| description | Radix Polygoni Multiflori (PM) is a well−known nootropic used in traditional Chinese medicine (TCM). Considering the efficacy and application discrepancy between raw (RPM) and processed PM (PPM), the similarities and differences between them in the treatment of vascular dementia (VaD) is intriguing. In this study, a VaD rat model was constructed by 2−vessel occlusion (2−VO). During 28 days of treatment, plasma was collected on days 7, 14, 21, and 28 after the start of dosing and the metabolic profile was analyzed by HPLC−MS/MS−based metabolomics. The Morris Water Maze Test, hematoxylin–eosin and Nissl staining, and biochemical analysis were used to assess cognitive function, pathogenic alterations and oxidative stress, respectively. RPM and PPM effectivelyreducedthe 2VO−induced cognitive impairment and mitigated histological alterations in hippocampus tissue. The 2−VO model significantly elevated MDA level and decreased SOD activity and GSH level, indicating severe oxidative stress, which could also be attenuated by RPM and PPM treatment. RPM outperformed PPM in decreasing MDA levels while PPM outperformed RPM in increasing GSH levels. Differential metabolites were subjected to Metabolite Set Enrichment Analysis (MSEA) and genes corresponding to proteins having interactions with metabolites were further annotated with Gene Ontology (GO). Both RPM and PPM ameliorated VaD−relevant vitamin B6 metabolism, pentose phosphate pathways, and taurine and hypotaurine metabolism. In addition, the metabolism of cysteine and methionine was regulated only by RPM, and riboflavin metabolism was modulated only by PPM. The results suggested that raw and processed PM had comparable efficacy in the treatment of VaD but also with some mechanistic differenece. |
| first_indexed | 2024-03-09T16:05:46Z |
| format | Article |
| id | doaj.art-0907712e24be4a1380a71a0c71a6ca3e |
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| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T16:05:46Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-0907712e24be4a1380a71a0c71a6ca3e2023-11-24T16:39:05ZengMDPI AGMetabolites2218-19892022-12-011212129710.3390/metabo12121297Comparative Investigation of Raw and Processed Radix Polygoni Multiflori on the Treatment of Vascular Dementia by Liquid Chromatograph−Mass Spectrometry Based Metabolomic ApproachFengye Wu0Yunlin Li1Wenya Liu2Ran Xiao3Benxing Yao4Mingzhe Gao5Di Xu6Junsong Wang7Center of Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, ChinaCenter of Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, ChinaCenter of Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, ChinaCenter of Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, ChinaCenter of Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, ChinaCenter of Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, ChinaCenter of Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, ChinaCenter of Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, ChinaRadix Polygoni Multiflori (PM) is a well−known nootropic used in traditional Chinese medicine (TCM). Considering the efficacy and application discrepancy between raw (RPM) and processed PM (PPM), the similarities and differences between them in the treatment of vascular dementia (VaD) is intriguing. In this study, a VaD rat model was constructed by 2−vessel occlusion (2−VO). During 28 days of treatment, plasma was collected on days 7, 14, 21, and 28 after the start of dosing and the metabolic profile was analyzed by HPLC−MS/MS−based metabolomics. The Morris Water Maze Test, hematoxylin–eosin and Nissl staining, and biochemical analysis were used to assess cognitive function, pathogenic alterations and oxidative stress, respectively. RPM and PPM effectivelyreducedthe 2VO−induced cognitive impairment and mitigated histological alterations in hippocampus tissue. The 2−VO model significantly elevated MDA level and decreased SOD activity and GSH level, indicating severe oxidative stress, which could also be attenuated by RPM and PPM treatment. RPM outperformed PPM in decreasing MDA levels while PPM outperformed RPM in increasing GSH levels. Differential metabolites were subjected to Metabolite Set Enrichment Analysis (MSEA) and genes corresponding to proteins having interactions with metabolites were further annotated with Gene Ontology (GO). Both RPM and PPM ameliorated VaD−relevant vitamin B6 metabolism, pentose phosphate pathways, and taurine and hypotaurine metabolism. In addition, the metabolism of cysteine and methionine was regulated only by RPM, and riboflavin metabolism was modulated only by PPM. The results suggested that raw and processed PM had comparable efficacy in the treatment of VaD but also with some mechanistic differenece.https://www.mdpi.com/2218-1989/12/12/1297Chinese materia medica processingRadix Polygoni Multiflorivascular dementiametabolomicsHPLC−MS/MS |
| spellingShingle | Fengye Wu Yunlin Li Wenya Liu Ran Xiao Benxing Yao Mingzhe Gao Di Xu Junsong Wang Comparative Investigation of Raw and Processed Radix Polygoni Multiflori on the Treatment of Vascular Dementia by Liquid Chromatograph−Mass Spectrometry Based Metabolomic Approach Metabolites Chinese materia medica processing Radix Polygoni Multiflori vascular dementia metabolomics HPLC−MS/MS |
| title | Comparative Investigation of Raw and Processed Radix Polygoni Multiflori on the Treatment of Vascular Dementia by Liquid Chromatograph−Mass Spectrometry Based Metabolomic Approach |
| title_full | Comparative Investigation of Raw and Processed Radix Polygoni Multiflori on the Treatment of Vascular Dementia by Liquid Chromatograph−Mass Spectrometry Based Metabolomic Approach |
| title_fullStr | Comparative Investigation of Raw and Processed Radix Polygoni Multiflori on the Treatment of Vascular Dementia by Liquid Chromatograph−Mass Spectrometry Based Metabolomic Approach |
| title_full_unstemmed | Comparative Investigation of Raw and Processed Radix Polygoni Multiflori on the Treatment of Vascular Dementia by Liquid Chromatograph−Mass Spectrometry Based Metabolomic Approach |
| title_short | Comparative Investigation of Raw and Processed Radix Polygoni Multiflori on the Treatment of Vascular Dementia by Liquid Chromatograph−Mass Spectrometry Based Metabolomic Approach |
| title_sort | comparative investigation of raw and processed radix polygoni multiflori on the treatment of vascular dementia by liquid chromatograph mass spectrometry based metabolomic approach |
| topic | Chinese materia medica processing Radix Polygoni Multiflori vascular dementia metabolomics HPLC−MS/MS |
| url | https://www.mdpi.com/2218-1989/12/12/1297 |
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