Establishment and characterization of murine models of asthma and subcutaneous immunotherapy for Humulus pollen allergy
Abstract Introduction Humulus pollen is an important cause of allergic asthma in East Asia. There have been some murine models for Humulus pollen allergy established by intraperitoneal (IP) sensitization and nasal drip stimulation, but they were not comprehensive enough. Here, we used atomized inhal...
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| Format: | Article |
| Language: | English |
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Wiley
2021-06-01
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| Series: | Immunity, Inflammation and Disease |
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| Online Access: | https://doi.org/10.1002/iid3.405 |
| _version_ | 1797831372158533632 |
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| author | Guang P. Xi Qian Zhang Jia Yin |
| author_facet | Guang P. Xi Qian Zhang Jia Yin |
| author_sort | Guang P. Xi |
| collection | DOAJ |
| description | Abstract Introduction Humulus pollen is an important cause of allergic asthma in East Asia. There have been some murine models for Humulus pollen allergy established by intraperitoneal (IP) sensitization and nasal drip stimulation, but they were not comprehensive enough. Here, we used atomized inhalation for challenge and compared the subcutaneous (SC) and IP sensitization routes to determine the optimal method to establish a model of asthma induced by Humulus pollen. Subsequently, we tried to develop a rapid subcutaneous immunotherapy (SCIT) model for Humulus allergy. Methods BALB/c Mice were sensitized through the SC or IP route, with respective reference to previously established sensitization methods and allergen dosing, and challenged with nebulized Humulus pollen extract to induce asthma. To compare the two sensitization methods, airway hyperresponsiveness (AHR), inflammatory cell infiltration, allergen‐specific serum immunoglobulin (Ig)E (sIgE) levels, cytokine levels, and lung histopathology were assessed. The effects of SCIT (once every other day for 16 days) on airway inflammation, AHR, sIgE, and allergen‐specific serum IgG2a (sIgG2a) levels were evaluated by using the model established in this study. Results Although mice sensitized by the SC or IP routes both showed AHR and airway inflammation, the SC route elicited significantly higher levels of sIgE, eosinophil inflammation, and T helper type 2 cytokines, compared with the IP route. SCIT in the treatment group significantly reduced the titers of sIgE, enhanced the titers of sIgG2a, and effectively alleviated pulmonary inflammation and AHR, compared with the vehicle group. Conclusions The SC route can be used to establish a murine model of Humulus pollen allergy that recapitulates the characteristics of clinical allergic asthma. Short‐term SCIT can significantly improve symptoms and pathophysiology in asthmatic mice. |
| first_indexed | 2024-04-09T13:52:00Z |
| format | Article |
| id | doaj.art-09089be508c34ce59fca89cb862276b0 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-04-09T13:52:00Z |
| publishDate | 2021-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-09089be508c34ce59fca89cb862276b02023-05-08T13:20:47ZengWileyImmunity, Inflammation and Disease2050-45272021-06-019244345510.1002/iid3.405Establishment and characterization of murine models of asthma and subcutaneous immunotherapy for Humulus pollen allergyGuang P. Xi0Qian Zhang1Jia Yin2Department of Allergy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences Peking Union Medical College Beijing ChinaDepartment of Allergy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences Peking Union Medical College Beijing ChinaDepartment of Allergy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences Peking Union Medical College Beijing ChinaAbstract Introduction Humulus pollen is an important cause of allergic asthma in East Asia. There have been some murine models for Humulus pollen allergy established by intraperitoneal (IP) sensitization and nasal drip stimulation, but they were not comprehensive enough. Here, we used atomized inhalation for challenge and compared the subcutaneous (SC) and IP sensitization routes to determine the optimal method to establish a model of asthma induced by Humulus pollen. Subsequently, we tried to develop a rapid subcutaneous immunotherapy (SCIT) model for Humulus allergy. Methods BALB/c Mice were sensitized through the SC or IP route, with respective reference to previously established sensitization methods and allergen dosing, and challenged with nebulized Humulus pollen extract to induce asthma. To compare the two sensitization methods, airway hyperresponsiveness (AHR), inflammatory cell infiltration, allergen‐specific serum immunoglobulin (Ig)E (sIgE) levels, cytokine levels, and lung histopathology were assessed. The effects of SCIT (once every other day for 16 days) on airway inflammation, AHR, sIgE, and allergen‐specific serum IgG2a (sIgG2a) levels were evaluated by using the model established in this study. Results Although mice sensitized by the SC or IP routes both showed AHR and airway inflammation, the SC route elicited significantly higher levels of sIgE, eosinophil inflammation, and T helper type 2 cytokines, compared with the IP route. SCIT in the treatment group significantly reduced the titers of sIgE, enhanced the titers of sIgG2a, and effectively alleviated pulmonary inflammation and AHR, compared with the vehicle group. Conclusions The SC route can be used to establish a murine model of Humulus pollen allergy that recapitulates the characteristics of clinical allergic asthma. Short‐term SCIT can significantly improve symptoms and pathophysiology in asthmatic mice.https://doi.org/10.1002/iid3.405Humulus pollenIP routemurine model of asthmaSC routesubcutaneous immunotherapy |
| spellingShingle | Guang P. Xi Qian Zhang Jia Yin Establishment and characterization of murine models of asthma and subcutaneous immunotherapy for Humulus pollen allergy Immunity, Inflammation and Disease Humulus pollen IP route murine model of asthma SC route subcutaneous immunotherapy |
| title | Establishment and characterization of murine models of asthma and subcutaneous immunotherapy for Humulus pollen allergy |
| title_full | Establishment and characterization of murine models of asthma and subcutaneous immunotherapy for Humulus pollen allergy |
| title_fullStr | Establishment and characterization of murine models of asthma and subcutaneous immunotherapy for Humulus pollen allergy |
| title_full_unstemmed | Establishment and characterization of murine models of asthma and subcutaneous immunotherapy for Humulus pollen allergy |
| title_short | Establishment and characterization of murine models of asthma and subcutaneous immunotherapy for Humulus pollen allergy |
| title_sort | establishment and characterization of murine models of asthma and subcutaneous immunotherapy for humulus pollen allergy |
| topic | Humulus pollen IP route murine model of asthma SC route subcutaneous immunotherapy |
| url | https://doi.org/10.1002/iid3.405 |
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