A Synbiotic Combination of Lactobacillus gasseri 505 and Cudrania tricuspidata Leaf Extract Prevents Stress-Induced Testicular Dysfunction in Mice
This study investigated the effects of a synbiotic combination (Syn) of Lactobacillus gasseri 505 (505) and Cudrania tricuspidata leaf extract (CT) on the hypothalamic-pituitary-gonadal axis in mice under chronic stress. Unpredictable chronic mild stress (UCMS) significantly increased the serum leve...
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| Format: | Article |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.835033/full |
| _version_ | 1818255158042165248 |
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| author | Jae Yeon Joung Whasun Lim Yeon Jeong Seo Jiyeon Ham Nam Su Oh Sae Hun Kim |
| author_facet | Jae Yeon Joung Whasun Lim Yeon Jeong Seo Jiyeon Ham Nam Su Oh Sae Hun Kim |
| author_sort | Jae Yeon Joung |
| collection | DOAJ |
| description | This study investigated the effects of a synbiotic combination (Syn) of Lactobacillus gasseri 505 (505) and Cudrania tricuspidata leaf extract (CT) on the hypothalamic-pituitary-gonadal axis in mice under chronic stress. Unpredictable chronic mild stress (UCMS) significantly increased the serum levels of corticosterone, however, treatment with Syn suppressed UCMS-induced increases. Histopathological analysis of the testes showed that these organs experienced some damage during UCMS, but this was repaired following treatment with Syn. Similarly, the transcription levels of gonadotropin-releasing hormone (GnRH), GnRH receptor, and gonadotropins, moreover, testicular development (i.e., Adam5, Adam29, and Spam1) - and steroidogenesis (i.e., Lhr, Egfr, and StAR) -related genes were significantly downregulated by UCMS. These UCMS-induced changes were inhibited by the administration of Syn, which was confirmed by the results of in situ hybridization analysis. These results suggest that the administration of Syn could attenuate the testicular dysfunctions induced by UCMS. |
| first_indexed | 2024-12-12T17:07:24Z |
| format | Article |
| id | doaj.art-090b52a8ef5c4239b90b98c5bb02acc7 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-12T17:07:24Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-090b52a8ef5c4239b90b98c5bb02acc72022-12-22T00:17:56ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-04-011310.3389/fendo.2022.835033835033A Synbiotic Combination of Lactobacillus gasseri 505 and Cudrania tricuspidata Leaf Extract Prevents Stress-Induced Testicular Dysfunction in MiceJae Yeon Joung0Whasun Lim1Yeon Jeong Seo2Jiyeon Ham3Nam Su Oh4Sae Hun Kim5Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South KoreaDepartment of Biological Sciences, Sungkyunkwan University, Suwon, South KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea Department of Biotechnology, Institute of Animal Molecular Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South KoreaDepartment of Food and Biotechnology, Korea University, Sejong, South KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South KoreaThis study investigated the effects of a synbiotic combination (Syn) of Lactobacillus gasseri 505 (505) and Cudrania tricuspidata leaf extract (CT) on the hypothalamic-pituitary-gonadal axis in mice under chronic stress. Unpredictable chronic mild stress (UCMS) significantly increased the serum levels of corticosterone, however, treatment with Syn suppressed UCMS-induced increases. Histopathological analysis of the testes showed that these organs experienced some damage during UCMS, but this was repaired following treatment with Syn. Similarly, the transcription levels of gonadotropin-releasing hormone (GnRH), GnRH receptor, and gonadotropins, moreover, testicular development (i.e., Adam5, Adam29, and Spam1) - and steroidogenesis (i.e., Lhr, Egfr, and StAR) -related genes were significantly downregulated by UCMS. These UCMS-induced changes were inhibited by the administration of Syn, which was confirmed by the results of in situ hybridization analysis. These results suggest that the administration of Syn could attenuate the testicular dysfunctions induced by UCMS.https://www.frontiersin.org/articles/10.3389/fendo.2022.835033/fullmale reproductive functionsynbioticsLactobacillus gasseri 505Cudrania tricuspidata leaf extractchronic stress |
| spellingShingle | Jae Yeon Joung Whasun Lim Yeon Jeong Seo Jiyeon Ham Nam Su Oh Sae Hun Kim A Synbiotic Combination of Lactobacillus gasseri 505 and Cudrania tricuspidata Leaf Extract Prevents Stress-Induced Testicular Dysfunction in Mice Frontiers in Endocrinology male reproductive function synbiotics Lactobacillus gasseri 505 Cudrania tricuspidata leaf extract chronic stress |
| title | A Synbiotic Combination of Lactobacillus gasseri 505 and Cudrania tricuspidata Leaf Extract Prevents Stress-Induced Testicular Dysfunction in Mice |
| title_full | A Synbiotic Combination of Lactobacillus gasseri 505 and Cudrania tricuspidata Leaf Extract Prevents Stress-Induced Testicular Dysfunction in Mice |
| title_fullStr | A Synbiotic Combination of Lactobacillus gasseri 505 and Cudrania tricuspidata Leaf Extract Prevents Stress-Induced Testicular Dysfunction in Mice |
| title_full_unstemmed | A Synbiotic Combination of Lactobacillus gasseri 505 and Cudrania tricuspidata Leaf Extract Prevents Stress-Induced Testicular Dysfunction in Mice |
| title_short | A Synbiotic Combination of Lactobacillus gasseri 505 and Cudrania tricuspidata Leaf Extract Prevents Stress-Induced Testicular Dysfunction in Mice |
| title_sort | synbiotic combination of lactobacillus gasseri 505 and cudrania tricuspidata leaf extract prevents stress induced testicular dysfunction in mice |
| topic | male reproductive function synbiotics Lactobacillus gasseri 505 Cudrania tricuspidata leaf extract chronic stress |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.835033/full |
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