Complete genome sequencing and comparative plasmid analysis of KPC-2-producing Klebsiella pneumoniae isolated from hospital sewage water in Japan
Objectives: The Klebsiella pneumoniae carbapenemase (blaKPC) gene is one of the most widespread carbapenemase genes in the world. However, there are few reports on KPC-producing bacteria in Japan. The aim of this study was therefore to investigate KPC-producing K. pneumoniae in Japan. Methods: A KPC...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716520303210 |
| _version_ | 1819107285542109184 |
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| author | Ryotaro Eda Shotaro Maehana Aki Hirabayashi Masaki Nakamura Takashi Furukawa Shinsuke Ikeda Kouji Sakai Fumiaki Kojima Kazunari Sei Masato Suzuki Hidero Kitasato |
| author_facet | Ryotaro Eda Shotaro Maehana Aki Hirabayashi Masaki Nakamura Takashi Furukawa Shinsuke Ikeda Kouji Sakai Fumiaki Kojima Kazunari Sei Masato Suzuki Hidero Kitasato |
| author_sort | Ryotaro Eda |
| collection | DOAJ |
| description | Objectives: The Klebsiella pneumoniae carbapenemase (blaKPC) gene is one of the most widespread carbapenemase genes in the world. However, there are few reports on KPC-producing bacteria in Japan. The aim of this study was therefore to investigate KPC-producing K. pneumoniae in Japan. Methods: A KPC-2-producingK. pneumoniae strain (KAM260) was isolated from hospital sewage water in Japan in 2018. The complete genome was determined by whole-genome sequencing. Subsequent comparative sequence analysis of the blaKPC-2-carrying plasmid was performed. Results: Klebsiella pneumoniae KAM260, belonging to sequence type 3026 (ST3026), harboured the blaKPC-2 gene in 114.6-kbp plasmid pKAM260_2 with IncFIB(pQIL) and IncFII(K) replicons. pKAM260_2 was highly identical to pKpQIL-like plasmids, which carry blaKPC genes and have spread worldwide. pKAM260_2 had functional conjugation-associated genes and was transferable to Escherichia coli. Conclusion: pKAM260_2, the self-transmissible plasmid carrying theblaKPC-2 gene, was detected from hospital sewage water in Japan and was characterised as a pKpQIL-like plasmid. This plasmid needs to be monitored in Japan in the future owing to its high diffusivity. |
| first_indexed | 2024-12-22T02:51:36Z |
| format | Article |
| id | doaj.art-090c4f68405740b78f1739ab9cf3e3bf |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-22T02:51:36Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-090c4f68405740b78f1739ab9cf3e3bf2022-12-21T18:41:22ZengElsevierJournal of Global Antimicrobial Resistance2213-71652021-03-0124180182Complete genome sequencing and comparative plasmid analysis of KPC-2-producing Klebsiella pneumoniae isolated from hospital sewage water in JapanRyotaro Eda0Shotaro Maehana1Aki Hirabayashi2Masaki Nakamura3Takashi Furukawa4Shinsuke Ikeda5Kouji Sakai6Fumiaki Kojima7Kazunari Sei8Masato Suzuki9Hidero Kitasato10Department of Environmental Microbiology, Graduate School of Medical Sciences, Kitasato University, Kanagawa, JapanDepartment of Environmental Microbiology, Graduate School of Medical Sciences, Kitasato University, Kanagawa, Japan; Department of Microbiology, School of Allied Health Sciences, Kitasato University, Kanagawa, JapanAntimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Environmental Microbiology, Graduate School of Medical Sciences, Kitasato University, Kanagawa, Japan; Department of Microbiology, School of Allied Health Sciences, Kitasato University, Kanagawa, Japan; Infection Control Research Center, Omura Satoshi Memorial Institute, Kitasato University, Tokyo, Japan; Regenerative Medicine and Cell Design Research Facility, Kanagawa, JapanDepartment of Environmental Hygiene, School of Allied Health Sciences, Kitasato University, Kanagawa, JapanDepartment of Orthopaedic Surgery, Zama General Hospital, Kanagawa, JapanDepartment of Veterinary Science, National Institute of Infectious Diseases, Tokyo, JapanRegenerative Medicine and Cell Design Research Facility, Kanagawa, Japan; Department of Pharmacology, School of Allied Health Sciences, Kitasato University, Kanagawa, JapanDepartment of Environmental Hygiene, School of Allied Health Sciences, Kitasato University, Kanagawa, JapanAntimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan; Corresponding author.Department of Environmental Microbiology, Graduate School of Medical Sciences, Kitasato University, Kanagawa, Japan; Department of Microbiology, School of Allied Health Sciences, Kitasato University, Kanagawa, Japan; Regenerative Medicine and Cell Design Research Facility, Kanagawa, Japan; Corresponding author at: Department of Environmental Microbiology, Graduate School of Medical Sciences, Kitasato University, Kanagawa, Japan.Objectives: The Klebsiella pneumoniae carbapenemase (blaKPC) gene is one of the most widespread carbapenemase genes in the world. However, there are few reports on KPC-producing bacteria in Japan. The aim of this study was therefore to investigate KPC-producing K. pneumoniae in Japan. Methods: A KPC-2-producingK. pneumoniae strain (KAM260) was isolated from hospital sewage water in Japan in 2018. The complete genome was determined by whole-genome sequencing. Subsequent comparative sequence analysis of the blaKPC-2-carrying plasmid was performed. Results: Klebsiella pneumoniae KAM260, belonging to sequence type 3026 (ST3026), harboured the blaKPC-2 gene in 114.6-kbp plasmid pKAM260_2 with IncFIB(pQIL) and IncFII(K) replicons. pKAM260_2 was highly identical to pKpQIL-like plasmids, which carry blaKPC genes and have spread worldwide. pKAM260_2 had functional conjugation-associated genes and was transferable to Escherichia coli. Conclusion: pKAM260_2, the self-transmissible plasmid carrying theblaKPC-2 gene, was detected from hospital sewage water in Japan and was characterised as a pKpQIL-like plasmid. This plasmid needs to be monitored in Japan in the future owing to its high diffusivity.http://www.sciencedirect.com/science/article/pii/S2213716520303210Klebsiella pneumoniaeblaKPC-2PlasmidHospital sewage waterJapan |
| spellingShingle | Ryotaro Eda Shotaro Maehana Aki Hirabayashi Masaki Nakamura Takashi Furukawa Shinsuke Ikeda Kouji Sakai Fumiaki Kojima Kazunari Sei Masato Suzuki Hidero Kitasato Complete genome sequencing and comparative plasmid analysis of KPC-2-producing Klebsiella pneumoniae isolated from hospital sewage water in Japan Journal of Global Antimicrobial Resistance Klebsiella pneumoniae blaKPC-2 Plasmid Hospital sewage water Japan |
| title | Complete genome sequencing and comparative plasmid analysis of KPC-2-producing Klebsiella pneumoniae isolated from hospital sewage water in Japan |
| title_full | Complete genome sequencing and comparative plasmid analysis of KPC-2-producing Klebsiella pneumoniae isolated from hospital sewage water in Japan |
| title_fullStr | Complete genome sequencing and comparative plasmid analysis of KPC-2-producing Klebsiella pneumoniae isolated from hospital sewage water in Japan |
| title_full_unstemmed | Complete genome sequencing and comparative plasmid analysis of KPC-2-producing Klebsiella pneumoniae isolated from hospital sewage water in Japan |
| title_short | Complete genome sequencing and comparative plasmid analysis of KPC-2-producing Klebsiella pneumoniae isolated from hospital sewage water in Japan |
| title_sort | complete genome sequencing and comparative plasmid analysis of kpc 2 producing klebsiella pneumoniae isolated from hospital sewage water in japan |
| topic | Klebsiella pneumoniae blaKPC-2 Plasmid Hospital sewage water Japan |
| url | http://www.sciencedirect.com/science/article/pii/S2213716520303210 |
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