Circulation of Bluetongue Virus Serotypes 1, 4, 8, 10 and 16 and Epizootic Hemorrhagic Disease Virus in the Sultanate of Oman in 2020–2021
The circulation of Bluetongue (BT) and Epizootic Hemorrhagic Disease (EHD) in the Middle East has already been reported following serological analyses carried out since the 1980s, mostly on wild ruminants. Thus, an EHD virus (EHDV) strain was isolated in Bahrain in 1983 (serotype 6), and more recent...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/6/1259 |
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| author | Emmanuel Bréard Lydie Postic Mathilde Gondard Cindy Bernelin-Cottet Aurélie Le Roux Mathilde Turpaud Pierrick Lucas Yannick Blanchard Damien Vitour Labib Bakkali-Kassimi Stéphan Zientara Wafaa Al Rawahi Corinne Sailleau |
| author_facet | Emmanuel Bréard Lydie Postic Mathilde Gondard Cindy Bernelin-Cottet Aurélie Le Roux Mathilde Turpaud Pierrick Lucas Yannick Blanchard Damien Vitour Labib Bakkali-Kassimi Stéphan Zientara Wafaa Al Rawahi Corinne Sailleau |
| author_sort | Emmanuel Bréard |
| collection | DOAJ |
| description | The circulation of Bluetongue (BT) and Epizootic Hemorrhagic Disease (EHD) in the Middle East has already been reported following serological analyses carried out since the 1980s, mostly on wild ruminants. Thus, an EHD virus (EHDV) strain was isolated in Bahrain in 1983 (serotype 6), and more recently, BT virus (BTV) serotypes 1, 4, 8 and 16 have been isolated in Oman. To our knowledge, no genomic sequence of these different BTV strains have been published. These same BTV or EHDV serotypes have circulated and, for some of them, are still circulating in the Mediterranean basin and/or in Europe. In this study, we used samples from domestic ruminant herds collected in Oman in 2020 and 2021 for suspected foot-and-mouth disease (FMD) to investigate the presence of BTV and EHDV in these herds. Sera and whole blood from goats, sheep and cattle were tested for the presence of viral genomes (by PCR) and antibodies (by ELISA). We were able to confirm the presence of 5 BTV serotypes (1, 4, 8, 10 and 16) and the circulation of EHDV in this territory in 2020 and 2021. The isolation of a BTV-8 strain allowed us to sequence its entire genome and to compare it with another BTV-8 strain isolated in Mayotte and with homologous BTV sequences available on GenBank. |
| first_indexed | 2024-03-11T01:49:41Z |
| format | Article |
| id | doaj.art-090e0b24475947da8c4ae6f8cdf9748d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T01:49:41Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-090e0b24475947da8c4ae6f8cdf9748d2023-11-18T13:01:14ZengMDPI AGViruses1999-49152023-05-01156125910.3390/v15061259Circulation of Bluetongue Virus Serotypes 1, 4, 8, 10 and 16 and Epizootic Hemorrhagic Disease Virus in the Sultanate of Oman in 2020–2021Emmanuel Bréard0Lydie Postic1Mathilde Gondard2Cindy Bernelin-Cottet3Aurélie Le Roux4Mathilde Turpaud5Pierrick Lucas6Yannick Blanchard7Damien Vitour8Labib Bakkali-Kassimi9Stéphan Zientara10Wafaa Al Rawahi11Corinne Sailleau12UMR 1161 VIROLOGIE, INRA, ENVA, ANSES, Laboratory for Animal Health, Paris Est University, 94701 Maisons-Alfort, FranceUMR 1161 VIROLOGIE, INRA, ENVA, ANSES, Laboratory for Animal Health, Paris Est University, 94701 Maisons-Alfort, FranceUMR 1161 VIROLOGIE, INRA, ENVA, ANSES, Laboratory for Animal Health, Paris Est University, 94701 Maisons-Alfort, FranceUMR 1161 VIROLOGIE, INRA, ENVA, ANSES, Laboratory for Animal Health, Paris Est University, 94701 Maisons-Alfort, FranceLaboratory of Ploufragan, ANSES, Unit of Viral Genetics and Biosafety, 22440 Ploufragan, FranceUMR 1161 VIROLOGIE, INRA, ENVA, ANSES, Laboratory for Animal Health, Paris Est University, 94701 Maisons-Alfort, FranceLaboratory of Ploufragan, ANSES, Unit of Viral Genetics and Biosafety, 22440 Ploufragan, FranceLaboratory of Ploufragan, ANSES, Unit of Viral Genetics and Biosafety, 22440 Ploufragan, FranceUMR 1161 VIROLOGIE, INRA, ENVA, ANSES, Laboratory for Animal Health, Paris Est University, 94701 Maisons-Alfort, FranceUMR 1161 VIROLOGIE, INRA, ENVA, ANSES, Laboratory for Animal Health, Paris Est University, 94701 Maisons-Alfort, FranceUMR 1161 VIROLOGIE, INRA, ENVA, ANSES, Laboratory for Animal Health, Paris Est University, 94701 Maisons-Alfort, FranceDepartment of Biology, College of Science, Sultan Qaboos University, Muscat P.C. 123, OmanUMR 1161 VIROLOGIE, INRA, ENVA, ANSES, Laboratory for Animal Health, Paris Est University, 94701 Maisons-Alfort, FranceThe circulation of Bluetongue (BT) and Epizootic Hemorrhagic Disease (EHD) in the Middle East has already been reported following serological analyses carried out since the 1980s, mostly on wild ruminants. Thus, an EHD virus (EHDV) strain was isolated in Bahrain in 1983 (serotype 6), and more recently, BT virus (BTV) serotypes 1, 4, 8 and 16 have been isolated in Oman. To our knowledge, no genomic sequence of these different BTV strains have been published. These same BTV or EHDV serotypes have circulated and, for some of them, are still circulating in the Mediterranean basin and/or in Europe. In this study, we used samples from domestic ruminant herds collected in Oman in 2020 and 2021 for suspected foot-and-mouth disease (FMD) to investigate the presence of BTV and EHDV in these herds. Sera and whole blood from goats, sheep and cattle were tested for the presence of viral genomes (by PCR) and antibodies (by ELISA). We were able to confirm the presence of 5 BTV serotypes (1, 4, 8, 10 and 16) and the circulation of EHDV in this territory in 2020 and 2021. The isolation of a BTV-8 strain allowed us to sequence its entire genome and to compare it with another BTV-8 strain isolated in Mayotte and with homologous BTV sequences available on GenBank.https://www.mdpi.com/1999-4915/15/6/1259bluetongueepizootic hemorrhagic diseasethe Sultanate of OmanBTV serotype 8 |
| spellingShingle | Emmanuel Bréard Lydie Postic Mathilde Gondard Cindy Bernelin-Cottet Aurélie Le Roux Mathilde Turpaud Pierrick Lucas Yannick Blanchard Damien Vitour Labib Bakkali-Kassimi Stéphan Zientara Wafaa Al Rawahi Corinne Sailleau Circulation of Bluetongue Virus Serotypes 1, 4, 8, 10 and 16 and Epizootic Hemorrhagic Disease Virus in the Sultanate of Oman in 2020–2021 Viruses bluetongue epizootic hemorrhagic disease the Sultanate of Oman BTV serotype 8 |
| title | Circulation of Bluetongue Virus Serotypes 1, 4, 8, 10 and 16 and Epizootic Hemorrhagic Disease Virus in the Sultanate of Oman in 2020–2021 |
| title_full | Circulation of Bluetongue Virus Serotypes 1, 4, 8, 10 and 16 and Epizootic Hemorrhagic Disease Virus in the Sultanate of Oman in 2020–2021 |
| title_fullStr | Circulation of Bluetongue Virus Serotypes 1, 4, 8, 10 and 16 and Epizootic Hemorrhagic Disease Virus in the Sultanate of Oman in 2020–2021 |
| title_full_unstemmed | Circulation of Bluetongue Virus Serotypes 1, 4, 8, 10 and 16 and Epizootic Hemorrhagic Disease Virus in the Sultanate of Oman in 2020–2021 |
| title_short | Circulation of Bluetongue Virus Serotypes 1, 4, 8, 10 and 16 and Epizootic Hemorrhagic Disease Virus in the Sultanate of Oman in 2020–2021 |
| title_sort | circulation of bluetongue virus serotypes 1 4 8 10 and 16 and epizootic hemorrhagic disease virus in the sultanate of oman in 2020 2021 |
| topic | bluetongue epizootic hemorrhagic disease the Sultanate of Oman BTV serotype 8 |
| url | https://www.mdpi.com/1999-4915/15/6/1259 |
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